20.500.12556/RUNG-2490
Bilirubin is an Endogenous Antioxidant in Human Vascular Endothelial Cells
Bilirubin is a standard serum biomarker of liver function. Inexplicably, it is inversely correlated with
cardiovascular disease risk. Given the role of endothelial dysfunction in originating cardiovascular
diseases, direct analysis of bilirubin in the vascular endothelium would shed light on these relationships.
Hence, we used high-performance liquid chromatography coupled with thermal lens spectrometric
detection and diode array detection for the determination of endogenous cellular IXα-bilirubin. To
confirm the isomer IXα-bilirubin, we used ultra-performance liquid chromatography coupled with a
high-resolution mass spectrometer using an electrospray ionization source, as well as tandem mass
spectrometric detection. We measured bilirubin in both arterial and venous rat endothelium (0.9–1.5
pmol mg−1 protein). In the human endothelial Ea.hy926 cell line, we demonstrated that intracellular
bilirubin (3–5 pmol mg−1 protein) could be modulated by either extracellular bilirubin uptake, or by
up-regulation of heme oxygenase-1, a cellular enzyme related to endogenous bilirubin synthesis.
Moreover, we determined intracellular antioxidant activity by bilirubin, with EC50 = 11.4 ± 0.2 nM, in
the range of reported values of free serum bilirubin (8.5–13.1 nM). Biliverdin showed similar antioxidant
properties as bilirubin. We infer from these observations that intra-endothelial bilirubin oscillates, and
may thus be a dynamic factor of the endothelial function.
Antioxidants
bilirubin
endothelial cells
true
true
false
Angleški jezik
Ni določen
Delo ni kategorizirano
2016-07-06 07:41:46
2016-07-07 04:53:06
2023-06-09 03:17:21
0000-00-00 00:00:00
2016
0
0
6
29240
6
2016
0000-00-00
Zaloznikova
NiDoloceno
NiDoloceno
0000-00-00
0000-00-00
0000-00-00
4431099
10.1038/srep29240
URN:SI:UNG:REP:LMXAR9KU
srep29240.pdf
srep29240.pdf
1
CED69F271B01D078CED44A3F56F46767
98a61978fe83a80b75ae305f2e78b75b7faf8d9c9b2a7424c9e08e3f42572251
14470aa4-05ce-11ee-9c48-5ef991fed68f
https://repozitorij.ung.si/Dokument.php?lang=slv&id=4279
srep29240-s1.pdf.zip
srep29240-s1.pdf.zip
1
79AE4DB1A612959F95433AAB71EE25D1
a406c88aa4500d983ca6f3db672853a6c58a04bd1291ba56bf9631391baa2779
14539e8e-05ce-11ee-9c48-5ef991fed68f
https://repozitorij.ung.si/Dokument.php?lang=slv&id=4280
Univerza v Novi Gorici
0
0
0