Hemichrome Determination by Thermal Lensing with Polyethylene Glycols for Signal Enhancement in Aqueous SolutionsGalimova, Viktoriya (Avtor)
Franko, Mladen (Avtor)
Proskurnin, Mikhail (Avtor)
Liu, Mingqiang (Avtor)
Hemoglobinhemikromoptotermična spektroskopijaetilenglikolspektrometrija s toplotnimi lečamiThe thermal lens technique is proposed for the determination of total hemoglobin in the form of reversible hemichrome. The conditions were optimized (concentration of sodium dodecyl sulfate, 2 mM) to attain the maximum sensitivity with the use of polyethylene glycols as signal enhancers. For polyethylene glycols with molecular weights 1500–35000 Da in a concentration range of 5–15% w/w (5–25 mM), the influence on thermal lens signal enhancement was estimated. It is shown that the use of 5% w/w polyethylene glycol 2000 provides the maximum increase in the thermal lens enhancement factor (by 40%) in comparison with unmodified aqueous solutions. The detection limit of iron(II) tris(1,10-phenanthrolinate) as a model system is 60 nM. Under these conditions, the thermal lens detection limit of hemichrome is 10 nM, which shows a 15-fold enhancement compared to spectro- photometry. Modification of the medium with polyethylene glycols decreases the limit of detection of hemichrome determination by 15% in comparison with unmodified aqueous solutions due to better reproducibility for the range of concentrations from 0.02 to 0.9 μM.20182018-04-15 22:38:49Delo ni kategorizirano3905COBISS_ID: 5136891DOI: 10.1080/00032719.2017.1391828NUK URN: URN:SI:UNG:REP:LX3R9ASKsl