Laser ablation-single-particle-inductively coupled plasma mass spectrometry as a multimodality bioimaging tool in nano-based omicsMetarapi, Dino (Avtor)
Elteren, Johannes Teun van (Avtor)
Šala, Martin (Avtor)
Vogel-Mikuš, Katarina (Avtor)
Arčon, Iztok (Avtor)
Šelih, Vid Simon (Avtor)
Kolar, Mitja (Avtor)
Hočevar, Samo B. (Avtor)
Laser ablationAg XANESbioimagingAdvanced data processing and visualization techniques were applied to laser ablation-single particleinductively
coupled plasma mass spectrometry (LA-sp-ICPMS) data obtained from imaging of roots of
sunflower (Helianthus annuus L.) which were exposed to ionic silver (Ag+). Highly multiplexed images with
tissue level resolution were generated, showing the uptake and transformation of Ag+ to silver
nanoparticles (AgNPs) in whole root cross-sections, and revealing that the size of biosynthesized AgNPs is
predisposed by the reducing power of particular root compartments. Various Ag+ and AgNP visualization
strategies are shown with special emphasis on spatially resolved information associated with the number
and spherical-equivalent size of individual AgNPs in user-selected root cross-sectional regions, including
statistical analysis of the AgNP distribution. Normally, this multimodality information can only be obtained
by combining the results of techniques from several complementary platforms.20212021-06-03 10:48:25Neznano6567COBISS_ID: 50633731UDK: 54ISSN pri članku: 2051-8153DOI: 10.1039/D0EN01134GNUK URN: URN:SI:UNG:REP:YRIZCYB0sl