11. Development of encapsulation techniques for the production and conservation of synthetic seeds in ornamental plantsYelda Ozden-Tokatli, Anna De Carlo, Fusun Gumusel, Sara Pignattelli, Maurizio Lambardi, 2008, original scientific article Abstract: The synthetic seed ("synseed") technology, initially developed through the encapsulation of somatic embryos, is now available for the use with non-embryogenic in vitro-derived explants, such as buds and nodal segments. For ornamental plants, to date there are few reports dealing with the production and the conservation of synseeds. Hence, the present study was conducted to develop an efficient encapsulation protocol for apical and axillary buds from various ornamental shrubs, i.e., oleander (Nerium oleander L.), photinia (Photinia fraseri Dress.), and lilac (Syringa vulgaris L.). For encapsulation, apical and axillary buds were excised, directly immersed in a Na-alginate solution, and then released dropwise in 100 mM CaCl2 center dot 2H(2)O for bead hardening. With photinia, best results were achieved when the synseeds were prepared using 3% Na-alginate, beads hardened for 30 min and germinated on hormone-free or BA-containing gelled MS medium. Thus, up to 92% of synseeds germinated and converted to shoots in a period of 10-11 days. The synthetic seeds of lilac, containing apical buds, showed a shorter germination time when 10-30 g 1(-1) sucrose was included in their "artificial endosperm". Successful medium-term conservation was then achieved with the storage of synthetic seeds at 4 degrees C in the dark on gelled MS medium, where up to 75% (oleander) and and 9 1 % (photinia) synseeds still germinated after 2 or 3 months of cold storage, respectively. Moreover, promising results have been obtained with the cryopreservation of photinia apical buds by means of the technique of "encapsulation-dehydration".
Keywords: conservation, cryopreservation, encapsulation, ornamentals, synthetic seeds
Published in RUNG: 20.04.2020; Views: 2787; Downloads: 0
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12. Role of Cu current collector on electrochemical mechanism of Mg–S batteryAna Robba, Maja Mežnar, Alen Vižintin, Jan Bitenc, Jernej Bobnar, Iztok Arčon, Anna Randon-Vitanova, Robert Dominko, 2020, original scientific article Abstract: Development of magnesium sulfur battery is accompanied with all known difficulties present in Li–S batteries,
however with even more limited choice of electrolytes. In the present work, the influence of current collector on
electrochemical mechanism was investigated in light of different reports where improved behavior was ascribed
to electrolyte. Notable differences in cycling behavior are reported when Al current collector is replaced by Cu
current collector independent of electrolyte. The initial reduction of sulfur follows similar reaction path no mater
of current collector, but formation of MgS can be in competition with formation of CuS in the presence of Cu
cations. With the subsequent cycling cells prepared from cathodes deposited on Cu current collector show
decrease in the voltage and formation of single plateau during cycling. The change corresponds to the
involvement of Cu into the reaction and formation of redox couple Mg/CuS as determined by Cu K-edge XANES
measurements. Corrosion of Cu foil is identified by SEM and serves as a source of Cu cations for the chemical
reaction between Cu and polysulfides. Mg/CuS redox couple shows improved cycling stability, but theoretical
energy density is severely reduced due to substitution of S with CuS as cathode active material.
Keywords: Magnesium
Sulfur
Rechargeable batteries
Current collector
Copper
Corrosion
Published in RUNG: 16.01.2020; Views: 2983; Downloads: 0
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13. Bacterial inclusion bodies are industrially exploitable amyloids.Ario De Marco, Neus Ferrer-Miralles, Elena Garcia-Fruitos, Anna Mitraki, Spela Peternel, Ursula Rinas, Mauricio Trujillo-Roldan, Norma Valdez-Cruz, Esther Vazquez, Antonio Villaverde, 2019, review article Keywords: inclusion bodies, soluble aggregates, functional proteins
Published in RUNG: 03.01.2019; Views: 3549; Downloads: 0
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14. The Road Movie : a Journey of Rebellion and Self-DiscoveryAnna Loi, 2018, undergraduate thesis Abstract: Film Ceste je pojav s širokimi družbenimi in kulturnimi posledicami. Pričujoče diplomsko delo
analizira značilnosti tega žanra, pri čemer upošteva nekatere najpomembnejše filme, ki so se
izkazali za ključne za razvoj žanra. V povojnem obdobju, ko se je družba spreminjala, ko se je
ponovno pojavil optimizem in ko se je življenjski standard povzpel do točke, da si je tudi srednji
sloj lahko privoščil potovanja brez posebnega razloga, je postal tako imenovani “road movie”
oziroma film ceste množični ameriški pojav, ki je bil v veliki meri je bil povezan tudi s
prostranostjo ZDA in z njenimi temeljnimi kulturnimi spremembami. Ta filmski žanr se je kmalu
razširil po vsem svetu in ustvaril lastna pravila in zgodovino, postal pa je tudi glasnik generacije 60.
let 20. stoletja. Do danes se filmi ceste še vedno dotikajo teme samospoznavanja in odkrivanja in
pogosto ne predstavljajo le fizičnega, temveč tudi metaforično potovanje vase, prav tako pa zelo
pogosto vsebujejo politično izjavo.
Keywords: potovanje, film, cesta, upor, samospoznavanje
Published in RUNG: 19.12.2018; Views: 4287; Downloads: 57
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15. A Mechanistic Study of Magnesium Sulfur BatteriesAna Robba, Alen Vižintin, Jan Bitenc, Gregor Mali, Iztok Arčon, Matjaž Kavčič, Matjaž Žitnik, Klemen Bučar, Giuliana Aquilanti, Charlotte Martineau-Corcos, Anna Randon-Vitanova, Robert Dominko, 2017, original scientific article Abstract: Magnesium sulfur batteries are considered as attractive energy storage devices due to the
abundance of electrochemically active materials and high theoretical energy density. Here we
report the mechanism of a Mg-S battery operation, which was studied in the presence of
simple and commercially available salts dissolved in a mixture of glymes. The electrolyte
offers high sulfur conversion into MgS in the first discharge with low polarization. The
electrochemical conversion of sulfur with magnesium proceeds through two well-defined
plateaus, which correspond to the equilibrium between sulfur and polysulfides (high-voltage
plateau) and polysulfides and MgS (low-voltage plateau). As shown by XANES, RIXS and
NMR studies, the end discharge phase involves MgS with Mg atoms in a tetrahedral
environment resembling the wurtzite structure, while chemically synthesized MgS crystalizes
in the rock-salt structure with octahedral coordination of magnesium.
Keywords: magnesium, sulfur, rechargeable batteries, XAS, NMR
Published in RUNG: 19.10.2017; Views: 4385; Downloads: 0
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17. The VPS4 component of the ESCRT machinery plays an essential role in HPV infectious entry and capsid disassemblyJustyna Broniarczyk, David Pim, Paola Massimi, Martina Bergant Marušič, Anna Gozdzicka-Jozefiak, Colin Crump, Lawrence Banks, 2017, original scientific article Abstract: Human Papillomavirus (HPV) infection involves multiple steps, from cell attachment, through endocytic trafficking towards the trans-Golgi network, and, ultimately, the entry into the nucleus during mitosis. An essential viral protein in infectious entry is the minor capsid protein L2, which engages different components of the endocytic sorting machinery during this process. The ESCRT machinery is one such component that seems to play an important role in the early stages of infection. Here we have analysed the role of specific ESCRT components in HPV infection, and we find an essential role for VPS4. Loss of VPS4 blocks infection with multiple PV types, suggesting an evolutionarily conserved critical step in infectious entry. Intriguingly, both L1 and L2 can interact with VPS4, and appear to be in complex with VPS4 during the early stages of virus infection. By using cell lines stably expressing a dominant-negative mutant form of VPS4, we also show that loss of VPS4 ATPase activity results in a marked delay in capsid uncoating, resulting in a defect in the endocytic transport of incoming PsVs. These results demonstrate that the ESCRT machinery, and in particular VPS4, plays a critical role in the early stages of PV infection.
Keywords: HPV, ESCRT machinery, infection
Published in RUNG: 08.05.2017; Views: 4424; Downloads: 271
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18. In silico generation of peptides by replica exchange Monte Carlo: Docking-based optimization of maltose-binding-protein ligandsAnna Russo, Pasqualina Liana Scognamiglio, Rolando Pablo Hong Enriquez, Carlo Santambrogio, Rita Grandori, Daniela Marasco, Antonio Giordano, Giacinto Scoles, Sara Fortuna, 2015, original scientific article Abstract: Short peptides can be designed in silico and synthesized through automated techniques, making them advantageous and versatile protein binders. A number of docking-based algorithms allow for a computational screening of peptides as binders. Here we developed ex-novo peptides targeting the maltose site of the Maltose Binding Protein, the prototypical system for the study of protein ligand recognition. We used a Monte Carlo based protocol, to computationally evolve a set of octapeptides starting from a polialanine sequence. We screened in silico the candidate peptides and characterized their binding abilities by surface plasmon resonance, fluorescence and electrospray ionization mass spectrometry assays. These experiments showed the designed binders to recognize their target with micromolar affinity. We finally discuss the obtained results in the light of further improvement in the ex-novo optimization of peptide based binders.
Keywords: peptides, docking, optimisation, computation, maltose binding protein, probe, ligand
Published in RUNG: 12.10.2016; Views: 3963; Downloads: 140
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20. Effect of Ce doping on the electrocaloric effect of Sr [sub] x Ba [sub] (1-x) Nb [sub] 2 O [sub] 6 single crystalsMatjaž Valant, Anna-Karin Axelsson, Neil McN. Alford, Florian Le Goupil, Tadeusz Łukasiewicz, Jan Dec, Andrey Berenov, 2014, original scientific article Keywords: dopiranje s cerijem, elektrokalorični pojavi, enojni kristali
Published in RUNG: 16.06.2016; Views: 4034; Downloads: 145
 Link to full text |
