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1.
Anaerobic biodegradation of raw and pre-treated brewery spent grain utilizing solid state anaerobic digestion
Bruno Zelić, Gregor Drago Zupančič, Mario Panjicko, 2015, original scientific article

Found in: osebi
Published: 16.06.2016; Views: 1503; Downloads: 63
URL Fulltext (0,00 KB)

2.
Anaerobic digestion of brewery spent grain as a mono-substrate in a two-stage anaerobic digestion using solid-state digestion reactor and granulated biomass reactor
Marina Tišma, Bruno Zelić, Romana Marinšek Logar, Mario Panjicko, Gregor Drago Zupančič, 2016, published scientific conference contribution (invited lecture)

Abstract: Anaerobic digestion of brewery spent grain as a mono-substrate was studied. Anaerobic digestion was phase separated in solid state anaerobic digestion reactor, where mostly microbiological hydrolysis and acidogenesis and granular biomass reactor where mostly methanogenesis was performed. The overall process exhibited total solids degradation efficiency between 73.6 and 80.4%. Average specific biogas production was 424±36 L/kg, whereas biomethane production was 230±34 L/kg of brewery spent grain total solids. Granular biomass after adaptation exhibited stabile operation at C-N ratios as low as 0.2 – 0.3, which is rare in anaerobic digestion. P-cresol as a degradation product was present in concentrations up to 45 mg/L and during the process successfully degraded. The excellent adaptability of the granular biomass is confirmed by 67% shift in bacterial and a 32% shift in archaeal community structure in granular biomass reactor after 198 days of successful operation.
Found in: osebi
Keywords: Anaerobic digestion, biogas production, brewery spent grain, microbial biomass, p-cresol degradation, solid state anaerobic digestion
Published: 21.10.2016; Views: 1821; Downloads: 0
.pdf Fulltext (968,03 KB)

3.
Biogas Production from Brewer’s Yeast Using an Anaerobic Sequencing Batch Reactor
Gregor Drago Zupančič, Mario Panjicko, Bruno Zelić, 2017, original scientific article

Abstract: Renewable energy sources are becoming increasingly important in the beverage and food industries. In the brewing industry, a significant percentage of the used raw materials finishes the process as secondary resource or waste. The research on the anaerobic digestion of brewer’s yeast has been scarce until recent years. One of the reasons for this is its use as a secondary resource in the food industry and as cattle feed. Additionally, market value of brewer’s yeast is higher than its energy value. Due to the increase of energy prices, brewer’s yeast has become of interest as energy substrate despite its difficult degradability in anaerobic conditions. The anaerobic co-digestion of brewer’s yeast and anaerobically treated brewery wastewater was studied using a pilot-scale anaerobic sequencing batch reactor (ASBR) seeded with granular biomass. The experiments showed very good and stable operation with an organic loading rate of up to 8.0 kg/(m3·day), and with a maximum achieved organic loading rate of 13.6 kg/(m3·day) in a single cycle. A specific biogas productivity of over 0.430 m3/kg of the total chemical oxygen demand (COD) inserted, and total COD removal efficiencies of over 90 % were achieved. This study suggests that the brewer’s yeast can be successfully digested in an ASBR without adverse effects on the biogas production from brewer's yeast/wastewater mixtures of up to 8 % (by volume). By using the brewer's yeast in the ASBR process, the biogas production from brewery wastewater could be increased by 50 %.
Found in: osebi
Keywords: anaerobic digestion, ASBR, biogas, brewer’s yeast
Published: 15.06.2017; Views: 1371; Downloads: 0
.pdf Fulltext (728,20 KB)

4.
Biogas production from brewery spent grain as a mono-substrate in a two-stage process composed of solid-state anaerobic digestion and granular biomass reactors
Mario Panjicko, Gregor Drago Zupančič, Romana Marinšek-Logar, Lijana Fanedl, Marina Tišma, Bruno Zelić, 2017, original scientific article

Abstract: Anaerobic digestion of brewery spent grain as a mono-substrate was studied. Brewery spent grain is a substrate consisting largely of cellulose, hemicellulose and lignin, which are difficult to degrade anaerobically, mostly due to the presence of degradation products, such as phenolic compounds, which cause process inhibition. Therefore, a two-stage system was used for anaerobic digestion. Anaerobic digestion was phase separated in a solid-state anaerobic digestion reactor, where microbiological hydrolysis and acidogenesis occurred and in a granular biomass reactor where mostly methanogenesis was performed. The overall process exhibited total solids degradation efficiency between 75.9 and 83.0 %. Average specific biogas production was 414±32 L/kg, whereas biomethane production was 224±34 L/kg of added total solids. Granular biomass after adaptation exhibited stable operation at substrate C/N ratios in range 0.16 – 4.68. p-cresol was present in concentrations up to 45 mg/L and during the process was successfully degraded by granular biomass. The excellent adaptability of granular biomass was confirmed by 68.2 % shift in bacterial and a 31.8 % shift in archaeal community structure in a granular biomass reactor. The structure of the bacterial community from granular biomass reactor and solid-state anaerobic digestion reactor remained 79.4 % similar at the end of the experiment, whereas archaeal community was only 31.6 % similar. The process exhibited stable operation for 198 days, which shows that brewery spent grain can be successfully anaerobically digested and used for biogas production.
Found in: osebi
Keywords: biogas production, brewery spent grain, C/N ratio, dry digestion, microbial biomass, solid-state anaerobic digestion
Published: 18.08.2017; Views: 1233; Downloads: 0
.pdf Fulltext (2,27 MB)

5.
Corn silage fungal-based solid-state pretreatment for enhanced biogas production in anaerobic co-digestion with cow manure
Marina Tišma, Mirela Planinc, Ana Bucić Kojić, Mario Panjičko, Gregor Drago Zupančič, Bruno Zelić, 2018, original scientific article

Abstract: The objective of this research was to use white-rot fungus Trametes versicolor for corn silage pretreatment and to investigate the effect of pretreatment on biogas productivity. Semi-continuous pilot-scale experiment, comprised of two experimental phases, was carried out. In the first phase, operational conditions of the full-scale biogas plant were reproduced at pilot-scale. In that phase, the reactor was daily fed with the mixture of cow manure, digestate from industrial postfermentor, corn grits and ensiled corn silage, and the average methane generation rate was 0.167 m3 CH4 per kgVS. In the second phase, corn grits and ensiled corn silage were replaced with corn silage pretreated with T. versicolor, and the average methane generation rate increased up to 0.236 m3 per CH4 kgVS. The results of this study suggest that application of fungal-based solid-state pretreated corn silage has positive effect on pH stability and increase the biogas productivity.
Found in: osebi
Keywords: Semi-continuous anaerobic co-digestion Fungal-based pretreatment Corn silage Cow manure
Published: 17.01.2018; Views: 776; Downloads: 0
.pdf Fulltext (785,43 KB)

6.
Primjena pivskog tropa u proizvodnji bioplina
Mario Panjicko, Gregor Drago Zupančič, Marina Tišma, Bruno Zelić, 2017, independent scientific component part or a chapter in a monograph

Abstract: Pivarska industrija je veliki generator otpadnih tokova. Pivski trop, kao jedan od tri glavna otpada toka, po kemijskom je sastavu lignocelulozni materijal. Zbog sve većeg porasta cijena energije i povećane brige za očuvanjem okoliša, u zadnje vrijeme se provode brojna istraživanja procesa anaerobne razgradnje (anaerobne digestije) pivskog tropa u svrhu proizvodnje bioplina. Bioplin proizveden prilikom procesa anaerobne razgradnje sastoji se najvećim dijelom od metana (CH4) i ugljikovog dioksida (CO2), s malim udjelima sumporovodika (H2S) i amonijaka (NH3) te plinova koji se mogu naći u tragovima, kao što su vodik (H2), dušik (N2), ugljikov monoksid (CO), zasićeni ili halogenirani ugljikovodici te kisik (O2). Osnovni problem primjene pivskog tropa u procesu anaerobne razgradnje u svrhu proizvodnje bioplina je njegov složen kemijski sastav. Strukturna kompleksnost lignina, njegova kemijska stabilnost i netopljivost posebice otežavaju proces anaerobne razgradnje. U ovom pregledu prikazane su osnove procesa anaerobne razgradnje, vrste reaktora za provedbu procesa anaerobne razgradnje te su opisane mogućnosti primjene pivskog tropa u procesu anaerobne razgradnje.
Found in: osebi
Keywords: anaerobna razgradnja, bioreaktori, lignoceluloza, bioplin, pivski trop
Published: 23.04.2018; Views: 959; Downloads: 0
.pdf Fulltext (6,31 MB)

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