11. Dispersion experiments in central London: The 2007 DAPPLE projectCurtis R Wood, Samantha J Arnold, Ahmed A Balogun, Janet F Barlow, Stephen E Belcher, Rex E Britter, Hong Cheng, Adrian Dobre, Justin J N Lingard, Damien Martin, Marina K Neophytou, Fredrik K Petersson, Alan G Robins, Dudley E. Shallcross, Robert J Smalley, James E Tate, Alison S Tomlin, Iain R. White, 2009, original scientific article Abstract: In the event of a release of toxic gas in the center of London, emergency services personnel would need to determine quickly the extent of the area contaminated. The transport of pollutants by turbulent flow within the complex streets and building architecture of London, United Kingdom, is not straightforward, and we might wonder whether it is at all possible to make a scientifically reasoned decision. Here, we describe recent progress from a major U.K. project, Dispersion of Air Pollution and its Penetration into the Local Environment (DAPPLE; information online at www.dapple.org.uk). In DAPPLE, we focus on the movement of airborne pollutants in cities by developing a greater understanding of atmospheric flow and dispersion within urban street networks. In particular, we carried out full-scale dispersion experiments in central London from 2003 through 2008 to address the extent of the dispersion of tracers following their release at street level. These measurements complemented previous studies because 1) our focus was on dispersion within the first kilometer from the source, when most of the material was expected to remain within the street network rather than being mixed into the boundary layer aloft; 2) measurements were made under a wide variety of meteorological conditions; and 3) central London represents a European, rather than North American, city geometry. Interpretation of the results from the full-scale experiments was supported by extensive numerical and wind tunnel modeling, which allowed more detailed analysis under idealized and controlled conditions. In this article, we review the full-scale DAPPLE methodologies and show early results from the analysis of the 2007 field campaign data.
Keywords: Air quality, Atmospheric thermodynamics, Dispersions, Experiments
Published in RUNG: 18.07.2019; Views: 3968; Downloads: 0
This document has many files! More... |
12. Short-range urban dispersion experiments using fixed and moving sourcesDudley E. Shallcross, Damien Martin, Catheryn S Price, Graham Nickless, Iain R. White, Fredrik Petterson, Rex E Britter, Marina K Neophytou, James Tate, Alison S Tomlin, Stephen E Belcher, Janet F Barlow, Alan Robins, 2009, original scientific article Abstract: Four perfluorocarbon tracer dispersion experiments were carried out in central London, United Kingdom in 2004. These experiments were supplementary to the dispersion of air pollution and penetration into the local environment (DAPPLE) campaign and consisted of ground level releases, roof level releases and mobile releases; the latter are believed to be the first such experiments to be undertaken. A detailed description of the experiments including release, sampling, analysis and wind observations is given. The characteristics of dispersion from the fixed and mobile sources are discussed and contrasted, in particular, the decay in concentration levels away from the source location and the additional variability that results from the non-uniformity of vehicle speed.
Keywords: dapple, perfluorocarbon, tracer, mobile source
Published in RUNG: 18.07.2019; Views: 2907; Downloads: 0
This document has many files! More... |
13. |
14. Recombinant nanobodies as cheap and customizable reagents for unicellular algae detectionElisa Mazzega, Ario De Marco, Marina Cabrini, Alfred Beran, published scientific conference contribution abstract Abstract: At the present, the identification of planktonic species in coastal water mainly relies on light microscopy
observations. This kind of analyses is performed by highly trained personnel, requires lab equipment and long
processing time. High-throughput and easy-to-perform methods are instead highly needed for routine costal and
ballast water monitoring.
Immuno-reagents are widely employed in the medical field for routine diagnostics, where they provide the necessary
sensitivity and specificity, as for example for cancer subtype characterization. Reagents of similar grade are so far not
widely available for both diagnostics and basic research of microalgae. We describe the first successful isolation of a
single-domain antibody (nanobody or VHH) from a pre-immune library, its engineering into application-ready
reagents, and its inexpensive production as recombinant fusion protein.
Alexandrium minutum was chosen as a model organism to test the feasibility of the procedure. The procedure foresees
the panning of a pre-immune phage library of VHHs that was used for in vitro selection against directly the target cells.
Monoclonal nanobodies specific for A. minutum cells were identified and optimized for recombinant production as
fusion with fluorescent proteins in bacterial hosts. Such fluorescently-tagged VHHs were validated by
immunofluorescence and cytofluorimetry for their selectivity by testing unicellular algal species that can be found in
the same environment of A. minutum. Two nanobodies were found to be highly specific for the target cells, were able
to bind also cysts of A. minutum and they gave no cross-reaction, even for a not-toxic strain of the closely related A.
tamutum.
Different tags can be then fused to the selected nanobodies and used instead of the fluorescent proteins to obtain a
reagent immediately applicable to further techniques, such as cell Enzyme Linked Immuno Sorbent Assay (ELISA) or
biosensor surface functionalization. The newly produced reagents can be applied for direct whole-cell detection in
seawater, bypassing the need of cell processing required for DNA or RNA diagnostics, and can be used for both alive
and fixed cells, guaranteeing the possibility to check old samples and to perform confirmatory morphological studies.
Keywords: Nanobodies, toxic algae, detection, recombinant reagent, naive library, phage display
Published in RUNG: 21.12.2018; Views: 3607; Downloads: 0
This document has many files! More... |
15. Spremljanje, ugotavljanje in zagotavljanje kakovosti : poročilo za študijsko leto 2017/2018 : Univerza v Novi Gorici, Fakulteta za znanosti o okolju.Iztok Arčon, Blaž Belec, Marina Artico, Tea Stibilj Nemec, Nivec Štefančič, Maja Terčon, Matjaž Valant, Sabina Zelinšček, treatise, preliminary study, study Keywords: samoevalvacijsko poročilo
Published in RUNG: 20.12.2018; Views: 3637; Downloads: 0
This document has many files! More... |
16. Spremljanje, ugotavljanje in zagotavljanje kakovosti : Poročilo za študijsko leto 2016/2018Blaž Belec, Iztok Arčon, Marina Artico, Renata Kop, Vanja Lesica Baša, Tea Stibilj Nemec, Nives Štefančič, Maja Terčon, Matjaž Valant, Sabina Zelinšček, treatise, preliminary study, study Keywords: samoevalvacijsko poročilo
Published in RUNG: 05.11.2018; Views: 3792; Downloads: 0
This document has many files! More... |
17. Primjena pivskog tropa u proizvodnji bioplinaMario Panjicko, Gregor Drago Zupančič, Marina Tišma, Bruno Zelić, 2017, independent scientific component part or a chapter in a monograph Abstract: Pivarska industrija je veliki generator otpadnih tokova. Pivski trop, kao jedan od tri glavna otpada toka, po kemijskom je sastavu lignocelulozni materijal. Zbog sve većeg porasta cijena energije i povećane brige za očuvanjem okoliša, u zadnje vrijeme se provode brojna istraživanja procesa anaerobne razgradnje (anaerobne digestije) pivskog tropa u svrhu proizvodnje bioplina.
Bioplin proizveden prilikom procesa anaerobne razgradnje sastoji se najvećim dijelom od metana (CH4) i ugljikovog dioksida (CO2), s malim udjelima sumporovodika (H2S) i amonijaka (NH3) te plinova koji se mogu naći u tragovima, kao što su vodik (H2), dušik (N2), ugljikov monoksid (CO), zasićeni ili halogenirani ugljikovodici te kisik (O2).
Osnovni problem primjene pivskog tropa u procesu anaerobne razgradnje u svrhu proizvodnje bioplina je njegov složen kemijski sastav. Strukturna kompleksnost lignina, njegova kemijska stabilnost i netopljivost posebice otežavaju proces anaerobne razgradnje.
U ovom pregledu prikazane su osnove procesa anaerobne razgradnje, vrste reaktora za provedbu procesa anaerobne razgradnje te su opisane mogućnosti primjene pivskog tropa u procesu anaerobne razgradnje.
Keywords: anaerobna razgradnja, bioreaktori, lignoceluloza, bioplin, pivski trop
Published in RUNG: 23.04.2018; Views: 5089; Downloads: 0
This document has many files! More... |
18. Univerza v Novi Gorici, Fakulteta za znanosti o okolju: Spremljanje, ugotavljanje in zagotavljanje kakovosti Poročilo za študijsko leto 2013/2014 z analizo vseh področij delovanja iz vseh let po zadnji akreditacijiIztok Arčon, Marina Artico, Janja Humar, Renata Kop, Veronika Piccinini, Urška Lavrenčič Štangar, Tea Stibilj Nemec, Lucija Vidrih, Sabina Zelinšček, 2014, treatise, preliminary study, study Keywords: zagotavljanje kakovosti, visoko šolstvo, akreditacija, univerza
Published in RUNG: 05.02.2018; Views: 3914; Downloads: 0
This document has many files! More... |
19. Corn silage fungal-based solid-state pretreatment for enhanced biogas production in anaerobic co-digestion with cow manureMarina Tišma, Mirela Planinc, Ana Bucić Kojić, Mario Panjičko, Gregor Drago Zupančič, Bruno Zelić, 2018, original scientific article Abstract: The objective of this research was to use white-rot fungus Trametes versicolor for corn silage pretreatment and to investigate the effect of pretreatment on biogas productivity. Semi-continuous pilot-scale experiment, comprised of two experimental phases, was carried out. In the first phase, operational conditions of the full-scale biogas plant were reproduced at pilot-scale. In that phase, the reactor was daily fed with the mixture of cow manure,
digestate from industrial postfermentor, corn grits and ensiled corn silage, and the average methane generation
rate was 0.167 m3 CH4 per kgVS. In the second phase, corn grits and ensiled corn silage were replaced with corn
silage pretreated with T. versicolor, and the average methane generation rate increased up to 0.236 m3 per CH4 kgVS. The results of this study suggest that application of fungal-based solid-state pretreated corn silage has positive
effect on pH stability and increase the biogas productivity.
Keywords: Semi-continuous anaerobic co-digestion
Fungal-based pretreatment
Corn silage
Cow manure
Published in RUNG: 17.01.2018; Views: 3890; Downloads: 0
This document has many files! More... |
20. |
