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1.
Enzyme-immobilized hierarchically porous covalent organic framework biocomposite for catalytic degradation of broad-range emerging pollutants in water
Nada Elmerhi, Khadega Al-Maqdi, Khawlah Athamneh, Abdul Khayum Mohammed, Tina Škorjanc, Felipe Gándara, Jesus Raya, Pascal Simon, Olivier Siri, Ali Trabolsi, 2023, original scientific article

Abstract: Efficient enzyme immobilization is crucial for the successful commercialization of large-scale enzymatic water treatment. However, issues such as lack of high enzyme loading coupled with enzyme leaching present challenges for the widespread adoption of immobilized enzyme systems. The present study describes the development and bioremediation application of an enzyme biocomposite employing a cationic macrocycle-based covalent organic framework (COF) with hierarchical porosity for the immobilization of horseradish peroxidase (HRP). The intrinsic hierarchical porous features of the azacalix[4]arene-based COF (ACA-COF) allowed for a maximum HRP loading capacity of 0.76 mg/mg COF with low enzyme leaching (<5.0%). The biocomposite, HRP@ACA-COF, exhibited exceptional thermal stability (~200% higher relative activity than the free enzyme), and maintained ~60% enzyme activity after five cycles. LCMSMS analyses confirmed that the HRP@ACA-COF system was able to achieve >99% degradation of seven diverse types of emerging pollutants (2-mercaptobenzothiazole, paracetamol, caffeic acid, methylparaben, furosemide, sulfamethoxazole, and salicylic acid)in under an hour. The described enzyme-COF system offers promise for efficient wastewater bioremediation applications.
Keywords: covalent organic frameworks, enzymes, emerging pollutants, water purification, biocomposite
Published in RUNG: 11.08.2023; Views: 726; Downloads: 6
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Opera in drama
Richard Wagner, 2013

Keywords: gledališka igra, dramsko pesništvo, glasbena umetnost
Published in RUNG: 15.04.2022; Views: 1267; Downloads: 0

5.
Spisi o Wagnerju
2015

Keywords: gledališka igra, dramsko pesništvo, glasbena umetnost, opere
Published in RUNG: 13.04.2022; Views: 1301; Downloads: 0

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Tristan
Gottfried von Straßburg, 2021

Keywords: srednjeveška epika, viteški romani, nemški pesniki, 12.-13.st.
Published in RUNG: 01.03.2022; Views: 1362; Downloads: 0

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