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1.
Selection criteria and engineering of nanobodies for oncological applications
Ario de Marco, invited lecture at foreign university

Abstract: Lecture relative to the biotechnological features of nanobodies
Found in: ključnih besedah
Summary of found: ...nanobodies, phage display, antibody engineering, panning...
Keywords: nanobodies, phage display, antibody engineering, panning
Published: 14.07.2016; Views: 1520; Downloads: 0
.pdf Fulltext (22,12 KB)

2.
Nanobodies for research & clinical applications
Ario de Marco, invited lecture at foreign university

Abstract: Nanobody technology evolved rapidly in the last years and now is available for applications in basic sciences as well as in diagnostics and therapy
Found in: ključnih besedah
Summary of found: ...nanobodies, antibody functionalization, phage display panning, recombinant antibody production...
Keywords: nanobodies, antibody functionalization, phage display panning, recombinant antibody production
Published: 11.10.2016; Views: 1801; Downloads: 0
.pdf Fulltext (773,47 KB)

3.
Nanobody technology: principles & applications
Ario De Marco, invited lecture at foreign university

Abstract: Antibodies possess unattainable capacity to bind selectively and at high affinity their cognates. For this reason they have been largely used in applications which rely on specific molecular recognition. Biosensors, nanoparticles, and even cells can be functionalized with antibodies for improving sensitivity and target specificity. However, conventional antibodies (IgGs) are large molecules (150 kDa) that are difficult to engineer. In the last years, antibody fragments have become more and more popular as an effective alternative and specifically nanobodies raised enthusiasm because of their minimal mass (14 kDa), high stability, relative similarity to human sequences, and simplified mutagenesis. Pre-immune nanobody libraries have the further advantage of enabling blind selection for antigens that can be used to discriminate between subpopulations of cells and vesicles. The panning can be performed directly on intact cells and the resulting binders are specific for the native antigen conformation
Found in: ključnih besedah
Summary of found: ...difficult to engineer. In the last years, antibody fragments have become more and more popular... ...between subpopulations of cells and vesicles. The panning can be performed directly on intact cells...
Keywords: Nanobodies, in vitro panning, cancer biomarkers, recombinant antibody engineering
Published: 03.05.2017; Views: 2029; Downloads: 0
.pdf Fulltext (37,92 KB)

4.
Advantages offered by the nanobody format when highly-specific reagents are needed for biotechnological applications
Ario de Marco, invited lecture at foreign university

Found in: ključnih besedah
Summary of found: ... Antibody panning, pre-immune phage display library, nanobody, tumor...
Keywords: Antibody panning, pre-immune phage display library, nanobody, tumor marker, bacterial display
Published: 18.05.2017; Views: 1223; Downloads: 0
.pdf Fulltext (2,23 MB)

5.
Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitors
Ronan Crepin, Gianluca Veggiani, Selma Djender, Anne Beugnet, Francois Planeix, Christophe Pichon, Sandrine Moutel, Sebastian Amigorena, Franck Pérez, Nicolae Ghinea, Ario De Marco, 2017, original scientific article

Abstract: Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to identify useful functional antibodies for further members of the GPCR class.
Found in: ključnih besedah
Summary of found: ...cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment... ...nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library...
Keywords: nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library
Published: 19.10.2017; Views: 1158; Downloads: 0
.pdf Fulltext (982,69 KB)

6.
Pre-immune nanobody collections and their biotechnological applications
Ario De Marco, invited lecture at foreign university

Found in: ključnih besedah
Summary of found: ...phage libraries, nanobodies, in vitro antibody panning...
Keywords: phage libraries, nanobodies, in vitro antibody panning
Published: 17.09.2018; Views: 590; Downloads: 0
.pdf Fulltext (54,45 KB)

7.
Nanobodies: a flexible technological platform
Ario de Marco, invited lecture at foreign university

Found in: ključnih besedah
Summary of found: ...Nanobodies, immunoreagents, recombinant antibody panning...
Keywords: Nanobodies, immunoreagents, recombinant antibody panning
Published: 17.09.2018; Views: 566; Downloads: 0
.pdf Fulltext (244,04 KB)

8.
In vitro and in silico approaches to obtain tailored reagents – Nanobodies as model
Ario De Marco, invited lecture at foreign university

Found in: ključnih besedah
Summary of found: ...Immunoreagents, nanobodies, in vitro panning, recombinant antibody production...
Keywords: Immunoreagents, nanobodies, in vitro panning, recombinant antibody production
Published: 21.09.2018; Views: 591; Downloads: 0
.pdf Fulltext (979,15 KB)

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