11. Biotechnological applications of nanobody pre-immune librariesArio De Marco, invited lecture at foreign university Found in: ključnih besedah
Summary of found: ... nanobodies, pre-immune libraries, in vitro selection, biomarkers, recombinant...
Keywords: nanobodies, pre-immune libraries, in vitro selection, biomarkers, recombinant proteins
Published: 16.05.2017; Views: 1604; Downloads: 0
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13. Quantification of Circulating Cancer Biomarkers via Sensitive Topographic Measurements on Single Binder NanoarraysElena Ambrosetti, Pamela Paoletti, Alessandro Bosco, Pietro Parisse, Denis Scaini, Elda Tagliabue, Ario de Marco, Loredana Casalis, 2017, original scientific article Found in: ključnih besedah
Summary of found: ...Biosensors, immuno-capture, nanobodies, HER2, cancer diagostics...
Keywords: Biosensors, immuno-capture, nanobodies, HER2, cancer diagostics
Published: 22.06.2017; Views: 1662; Downloads: 109
Fulltext (4,49 MB) |
14. Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitorsRonan Crepin, Gianluca Veggiani, Selma Djender, Anne Beugnet, Francois Planeix, Christophe Pichon, Sandrine Moutel, Sebastian Amigorena, Franck Pérez, Nicolae Ghinea, Ario De Marco, 2017, original scientific article Abstract: Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity
and capacity to recognize their native antigen are critical characteristics for their in vivo application.
Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and
spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody
suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe
the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly
panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii)
show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope
with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules
in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to
identify useful functional antibodies for further members of the GPCR class.
Found in: ključnih besedah
Keywords: nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library
Published: 19.10.2017; Views: 1523; Downloads: 0
Fulltext (982,69 KB) |
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17. Isolation of anti‑extra‑cellular vesicle single‑domain antibodies by direct panning on vesicle‑enriched fractionsMilica Popovic, Elisa Mazzega, Barbara Toffoletto, Ario De Marco, 2018, original scientific article Found in: ključnih besedah
Summary of found: ...exosomes, nanobodies, tumor cells...
Keywords: exosomes, nanobodies, tumor cells
Published: 15.01.2018; Views: 1424; Downloads: 88
Fulltext (2,98 MB) |
18. Binding affinity prediction of nanobody–protein complexes by scoring of molecular dynamics trajectoriesMiguel Soler, Sara Fortuna, Ario de Marco, Alessandro Laio, 2018, original scientific article Found in: ključnih besedah
Summary of found: ...in silico modeling, nanobodies, binding affinity prediction...
Keywords: in silico modeling, nanobodies, binding affinity prediction
Published: 15.01.2018; Views: 1470; Downloads: 0
Fulltext (2,45 MB) |
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