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1.
Novel applications of oxygenic photosynthetic organisms
Danijel Stojković, 2015, doctoral dissertation

Abstract: Inspired by natural microorganisms that possess a rigid cell wall to protect them in harsh conditions, individual cells of Chlamydomonas reinhardtii were artificially encapsulated with different materials and hydrogen production was enhanced. The first attempt was to cover the cells with hydrated silicon dioxide, which is naturally formed by diatoms. The cells covered with silica were not able to produce hydrogen, but the improved TiO2-encapsualtion of Chlamydomonas reinhardtii was found to positively affect their hydrogen production under sulfur-deprived conditions. It was shown that incubation of the cells in the dark before exposure to light was necessary in order to overcome the toxic effects of the (RKK)4D8 catalytic peptide that is essential for TiO2 formation. The TiO2-encapsulated cells were able to produce H2 with about double efficiency during 5-day period compared to non-encapsulated cells. The light-to-H2 conversion efficiency of TiO2-encapsulated Chlamydomonas reinhardtii system was estimated to be more than 4 % under optimized conditions. The encapsulation with platinum led us to discover the bioreductive properties of Chlamydomonas reinhardtii. Beside the homogeneous Pt reduction carried out by the algae, the most surprising discovery was the possibility to use algae culture for targeted heterogeneous nucleation. An important discovery was that is possible to control the nucleation mechanism and redirect predominantly homogeneous into fully heterogeneous nucleation.
Found in: ključnih besedah
Summary of found: ...than 4 % under optimized conditions. The encapsulation with platinum led us to discover the...
Keywords: photosynthesis, H2 production, TiO2, encapsulation, Chlamydomonas reinhardtii, platinum reduction
Published: 01.04.2015; Views: 4418; Downloads: 130
.pdf Fulltext (23,38 MB)
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2.
Development of encapsulation techniques for the production and conservation of synthetic seeds in ornamental plants
Yelda Ozden-Tokatli, Anna De Carlo, Fusun Gumusel, Sara Pignattelli, Maurizio Lambardi, 2008, original scientific article

Abstract: The synthetic seed ("synseed") technology, initially developed through the encapsulation of somatic embryos, is now available for the use with non-embryogenic in vitro-derived explants, such as buds and nodal segments. For ornamental plants, to date there are few reports dealing with the production and the conservation of synseeds. Hence, the present study was conducted to develop an efficient encapsulation protocol for apical and axillary buds from various ornamental shrubs, i.e., oleander (Nerium oleander L.), photinia (Photinia fraseri Dress.), and lilac (Syringa vulgaris L.). For encapsulation, apical and axillary buds were excised, directly immersed in a Na-alginate solution, and then released dropwise in 100 mM CaCl2 center dot 2H(2)O for bead hardening. With photinia, best results were achieved when the synseeds were prepared using 3% Na-alginate, beads hardened for 30 min and germinated on hormone-free or BA-containing gelled MS medium. Thus, up to 92% of synseeds germinated and converted to shoots in a period of 10-11 days. The synthetic seeds of lilac, containing apical buds, showed a shorter germination time when 10-30 g 1(-1) sucrose was included in their "artificial endosperm". Successful medium-term conservation was then achieved with the storage of synthetic seeds at 4 degrees C in the dark on gelled MS medium, where up to 75% (oleander) and and 9 1 % (photinia) synseeds still germinated after 2 or 3 months of cold storage, respectively. Moreover, promising results have been obtained with the cryopreservation of photinia apical buds by means of the technique of "encapsulation-dehydration".
Found in: ključnih besedah
Keywords: conservation, cryopreservation, encapsulation, ornamentals, synthetic seeds
Published: 20.04.2020; Views: 168; Downloads: 0
.pdf Fulltext (3,94 MB)

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