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1.
Biotechnological applications of nanobody pre-immune libraries
Ario De Marco, invited lecture at foreign university

Found in: ključnih besedah
Keywords: nanobodies, pre-immune libraries, in vitro selection, biomarkers, recombinant proteins
Published: 16.05.2017; Views: 1258; Downloads: 0
.pdf Fulltext (331,92 KB)

2.
Nanobodies as biotechnological tools
Ario de Marco, invited lecture at foreign university

Found in: ključnih besedah
Keywords: nanobodies, pre-immune phage library, recombinant expression, biomarkers, tumors
Published: 17.05.2017; Views: 1294; Downloads: 0
.pdf Fulltext (290,82 KB)

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Nuovi modelli di ricerca: oncologia comparativa per lo studio dei sarcomi
Ario de Marco, unpublished invited conference lecture

Found in: ključnih besedah
Keywords: Comparative oncology, sarcoma, canine model, immune-reagents
Published: 10.10.2017; Views: 1079; Downloads: 0
.pdf Fulltext (173,81 KB)

6.
Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitors
Ronan Crepin, Gianluca Veggiani, Selma Djender, Anne Beugnet, Francois Planeix, Christophe Pichon, Sandrine Moutel, Sebastian Amigorena, Franck Pérez, Nicolae Ghinea, Ario De Marco, 2017, original scientific article

Abstract: Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to identify useful functional antibodies for further members of the GPCR class.
Found in: ključnih besedah
Keywords: nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library
Published: 19.10.2017; Views: 1159; Downloads: 0
.pdf Fulltext (982,69 KB)

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