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Najdeno v: osebi
Ključne besede: channels, TRP receptors, cell membrane
Objavljeno: 03.03.2021; Ogledov: 982; Prenosov: 0
Polno besedilo (2,10 MB)

Opis: This study provides information on mercury (Hg) localization, speciation and ligand environment in edible mushrooms: Boletus edulis, B. aereus and Scutiger pes-caprae collected at non-polluted and Hg polluted sites, by LA-ICP-MS, SR-μ-XRF and Hg L3-edge XANES and EXAFS. Mushrooms (especially young ones) collected at Hg polluted sites can contain more than 100 μg Hg g−1 of dry mass. Imaging of the element distribution shows that Hg accumulates mainly in the spore-forming part (hymenium) of the cap. Removal of hymenium before consumption can eliminate more than 50% of accumulated Hg. Mercury is mainly coordinated to di-thiols (43–82%), followed by di-selenols (13–35%) and tetra-thiols (12–20%). Mercury bioavailability, as determined by feeding the mushrooms to Spanish slugs (known metal bioindicators owing to accumulation of metals in their digestive gland), ranged from 4% (S. pes-caprae) to 30% (B. aereus), and decreased with increasing selenium (Se) levels in the mushrooms. Elevated Hg levels in mushrooms fed to the slugs induced toxic effects, but these effects were counteracted with increasing Se concentrations in the mushrooms, pointing to a protective role of Se against Hg toxicity through HgSe complexation. Nevertheless, consumption of the studied mushroom species from Hg polluted sites should be avoided.
Najdeno v: osebi
Ključne besede: edible mushrooms, HgSe complex, imaging of elemental distribution, LA-ICP-MS, alpha-XRF, XAS
Objavljeno: 24.10.2019; Ogledov: 2182; Prenosov: 0
Polno besedilo (1,79 MB)

Opis: According to current models, dimeric DNA Polymerases coordinate the replication of DNA leading and lagging strands. However, it was recently shown that trimeric DNA Polymerases, assembled in vitro, replicate the lagging strand more efficiently than dimeric replicases. Here we show that the τ, α, ε, and θ subunits of Escherichia coli DNA Polymerase III can be assembled in vivo, yielding the trimeric τ3α3ε3θ3 complex. Further, we propose a molecular model of this complex, whose catalytic action was investigated using model DNA substrates. Our observations indicate that trimeric DNA replicases reduce the gap between leading and lagging strand synthesis.
Najdeno v: osebi
Ključne besede: Trimeric DNA Polymerase, E. coli, DNA Polymerase III, τ3α3ε3θ3 complex, Triple DNA extension
Objavljeno: 27.01.2021; Ogledov: 1047; Prenosov: 0
Polno besedilo (1,85 MB)

Najdeno v: osebi
Ključne besede: nano-composites, polymers, thermal insulation
Objavljeno: 13.07.2021; Ogledov: 957; Prenosov: 0

Najdeno v: osebi
Ključne besede: meter, memory, Italian poetry, Dante
Objavljeno: 05.11.2021; Ogledov: 568; Prenosov: 19
Polno besedilo (0,00 KB)
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