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1.
Relocation and Hair Cortisol Concentrations in New Zealand White Rabbits.
Alberto Prandi, Marco Stebel, Federico Canavese, Marta Montillo, Mirco Corazzin, Antonella Comin, Tanja Peric, 2016, izvirni znanstveni članek

Opis: To investigate how long relocation modified hair cortisol concentrations in New Zealand white rabbits, 19 rabbits were subjected to a change in their breeding facility at the beginning of the trial and then were kept under stable environmental conditions. Hair samples were collected at the time of arrival to the nonhuman animal facility and at 40-day intervals from the same skin area for up to 440 days after the animals' arrival to the facility. A period effect on the hair cortisol concentration was found (p < .01). The transfer of the rabbits to the new facility might have induced an increase in the hypothalamic-pituitary-adrenal axis activity (p < .01). A second increase in hair cortisol concentration (p < .01) occurred at 320 days, after a change of personnel at the facility that occurred at 280 days, which was the only environmental change. The relocation of rabbits to the facility resulted in a stress response leading to elevated cortisol levels. The effect of relocation on mean cortisol concentrations was exhausted within 120 days when all environmental factors were kept stable.
Najdeno v: osebi
Ključne besede: Cortisol, HPA axis, hair, rabbit, relocation
Objavljeno: 21.07.2016; Ogledov: 1277; Prenosov: 0

2.
EFFECT OF DIET ON HAIR CORTISOL AND DHEA CONCENTRATIONS IN MOUSE
Alberto Prandi, Mirco Corazzin, Silvia Gazzin, Tanja Peric, Marta Montillo, Antonella Comin, objavljeni povzetek znanstvenega prispevka na konferenci

Opis: Obesity and MetS (Metabolic Syndrome) are both linked to persistent long-term hormonal and metabolic changes. In most of the studies, cortisol (C) and dehydroepiandrosterone (DHEA) concentrations have been measured in obese and normal-weight subjects, obtaining heterogeneous results. Plasma, saliva and urine, matrices that represent timepoint or short-term steroids exposure, were used for these studies. The aim was to study C, DHEA and C/DHEA ratio of mice pups in the hair, matrix capable of providing cumulative hormonal exposure. Sixty C57Bl/6 mice pups (30 males and 30 females) were housed in a temperature-controlled environment (22±2°C) and on a 12h light/dark schedule, under ad-libitum access to food and water for 16 weeks (welfare: Italian Law Decree 116-92 and EC Directive 86-609-EEC). Control and experimental diets were offered immediately after weaning (3 weeks old pups), for 16 weeks. Four experimental checkpoints were established (T1: 4 weeks, T2: 8 weeks, T3: 12 weeks and T4: 16 weeks of diet). T3 and T4 showed the hormonal concentrations of pubertal animals. Twentyseven pups (13 females and 14 males) were randomly group-housed in cages (6 for T1, 5 for T2, 6 for T3, 10 for T4) and assigned to control diet (CTRL: D12328, Research Diets, New Brunswick, NJ). 33 pups (17 females and 16 males) were randomly group-housed in cages (5 for T1, 6 for T2 and T3, 16 for T4) and assigned to the HFHC diet (HFHC: D12331, Research Diets, New Brunswick, NJ - plus 42g/L fructose/sucrose in drinking water). At each experimental checkpoint, all the animals of one cage for each experimental group were suppressed. Hair strands were carefully cut with scissors as close as possible to the skin from the back of the mice, paying attention to not to wound the animals. Hair was stored in an envelope at RT in a dry room until use. C and DHEA hair concentrations was measured by a solid-phase microtiter RIA assay (Peric et al., 2016 adapted in the mouse). Only at 8 weeks the HFHC group showed significantly higher C concentrations than the CTRL group (1.56±0.06 vs 1.92±0.130 pg/mg; P<0.05). DHEA concentrations were significantly reduced in the HFHC group than the CTRL group at 4 (114.64±13.93 vs 69.08±5.33 pg/mg; P<0.05), 8 (71.67±7.08 vs 50.38±4.74 pg/mg; P<0.05), 12 (73.27±8.29 vs 41.59±1.60 pg/mg; P<0.01) and 16 weeks (65.26±3.35 vs 55.05±1.84 pg/mg; P<0.05). The C/DHEA ratio was significantly increased in the HFHC than the CTRL group, at 8 (0.023±0.002 vs 0.040±0.005; P<0.01), 12 (0.024±0.003 vs 0.036±0.004; P<0.05) and 16 weeks (0.019±0.001 vs 0.025±0.002; P<0.01). The gender effect was not significant. Taking into account the lag time required for the hair emersion from the skin (about 1 week), the significant stimulation of the C at 8 weeks in the HFHC group refers to its chronic elevation from 5 to 7 weeks of treatment when the animals were not pubertal. Conversely, DHEA shows a chronic reduction in obese mice leading to consider an independent adrenal regulation of C and DHEA, both stimulated by ACTH. Research was supported by the Project CBM (MIUR DM60643). Peric et al. 2016 J Appl Anim Welf Sci 18:1-8.
Najdeno v: osebi
Ključne besede: DIET, HAIR, CORTISOL, DHEA, MOUSE
Objavljeno: 21.07.2016; Ogledov: 1588; Prenosov: 0
.pdf Polno besedilo (3,65 MB)

3.
Hair cortisol and testosterone concentrations and semen production of Bos taurus bulls
Mirco Corazzin, Maria Cristina Veronesi, Antonella Comin, Marta Montillo, Tanja Peric, Giuseppe Stradaioli, Alberto Prandi, 2017, izvirni znanstveni članek

Opis: The experiment was aimed to evaluate the usefulness of hair cortisol concentrations (HCC) in revealing the activation of the hypothalamic-pituitary-adrenal (HPA) axis due to the passage of young bulls from genetic rearing stations to artificial insemination (AI) centre and the influence of this passage on hair testosterone concentrations (HTC). Hair samples on 33 yearling bulls were collected at the time of arrival (ST1) to the AI centre and 3 more hair samples (ST2, ST3, ST4) were collected at monthly intervals. After the end of quarantine each animal was submitted to the standard procedures for collection and production of semen. Overall HCC were significantly affected by period of collection (p<.01). In particular, HCC was higher in samples obtained after finishing the quarantine period (ST2) compared with those obtained on ST1. After ST2, the HCC decreased reaching at ST4 a value similar to that recorded at ST1. An effect of sampling time on HTC was not found (p>.05). The effect of breed was observed only for HTC that was higher in PRI than BS (p<.01). The HCC group have not influenced the semen variables (p>.05). On the contrary, the effect of breed was evident. The results of this study replicated the effects of a major environmental stressor on mean cortisol concentrations assessed in hair, and add to the growing body of evidence that HCC is an effective and simply collected marker for long-term activity of the HPA system in response to persistent environmental challenge.
Najdeno v: osebi
Ključne besede: Hair, bulls, cortisol, testosterone, semen
Objavljeno: 20.03.2017; Ogledov: 1223; Prenosov: 0
.pdf Polno besedilo (1,71 MB)

4.
HAIR CORTISOL LEVEL IN THE GOLDEN JACKAL (CANIS AUREUS MOREOTICUS I. GEOFFROY SAINT HILAIRE, 1835) IN RELATION TO THE SAMPLE CONSERVATION, SEX AND ECOLOGICAL CHARACTERISTICS.
Stefano Filacorda, Veronica Maran, Tanja Peric, Stefano Pesaro, Marta Montillo, 2017, objavljeni povzetek znanstvenega prispevka na konferenci

Opis: Hair cortisol analysis is a complementary method for monitoring HPA axis activity, capturing systemic cortisol exposure over longer periods of time. We have tested the hair cortisol concentration on 16 specimens of Golden jackal (Canis aureus moreoticus): 5 from frozen animals, 10 from stuffed animals and 1 taken from injured animal during recovery in an Animal Care Center; the samples came from Italy (Friuli Venezia Giulia and Veneto Region) and Croatia (Istria and Dalmatian region). We have studied the hair cortisol concentration by RIA method in respect to conservation method, sex and areas of origin, with non parametric tests and mixed model. The hair cortisol concentration (values expressed in pg/mg) from the frozen animals (mean=3.38 SD=3.13, n=5) was not different in respect to the stuffed samples (mean=2.81 SD=2.72, n=9), also the sex has not showed any statistical effect (males mean=1.96 SD=0.63 n=9; females mean=4.56 SD=3.99 n=6). The hair cortisol concentration in respect to the sites of origin of samples, dispersal and core areas was not different (dispersal areas: mean=1.56 SD=0.77 n=5, core areas: mean=3.72 SD=3.14 n=10). The use of mixed model to detect the effect of areas (core and dispersal areas) and interaction between sex and areas has showed the presence of significative higher hair concentrations of cortisol in core areas for the females (mean=6.27 SD=3.84 n=4)in respect to the males (mean=2.02 SD=0.52 n=6). The concentration of hair cortisol of sick and injured animal recovered in the Animal Care Center was higher (> of 12 pg/mg) in respect to the other samples. Hair sample can be used to develop a clearer understanding of the interrelationship of health and physiology, mobility and social behaviour.
Najdeno v: osebi
Ključne besede: hair, Canis aureus, cortisol
Objavljeno: 19.07.2017; Ogledov: 1689; Prenosov: 0
.pdf Polno besedilo (1,91 MB)

5.
DEHYDROEPIANDROSTERONE SULFATE CLAWS CONCENTRATIONS IN DOGS FROM BIRTH TO 30 DAYS OF AGE: PRELIMINARY RESULTS.
Jasmine Fusi, Marta Montillo, Barbara Bolis, Alessandro Rota, Tanja Peric, Maria Cristina Veronesi, 2017, objavljeni povzetek znanstvenega prispevka na konferenci

Opis: Similarly to all other species, also in the dog improvements in the knowledge on perinatology are mandatory for a better management of newborns, mainly aimed to reduce the impact of perinatal mortality. However, until recently, the study of canine perinatology was limited mainly because of the invasiveness of many investigation procedures, such as repeated blood sampling. In recent times, the claws/nails were proved to be a useful, non invasive, matrix for long time-frame retrospective hormone concentrations analysis also in babies and puppies [1,2], providing a suitable matrix for perinatal long-term hormonal changes studies. The last intrauterine foetal stage of development and the neonatal period represent the most challenging phases for the mammals offspring. It was demonstrated that the activation of the fetal hypothalamic-pituitary-adrenal (HPA) axis leads to dehydroepiandrosterone (DHEA) and DHEA sulfate (DHEA-S) secretion, the major fetal steroids. Thus, DHEA-S measured at birth in newborns could be considered as a marker of offspring HPA axis activity, under the maternal influence. This study was aimed to assess the DHEA-S concentrations in newborn puppies claws, collected at birth and at 30 days of age, and to evaluate the possible influence of age, gender and type of birth on DHEA-S claws accumulation. The study was performed on 58 large purebred, normal, healthy, viable (Apgar≥7) puppies, 31 males and 27 females, born by vaginal spontaneous (N=22) or caesarean (N=36) parturition. DHEA-S was analysed by RIA. The mean ± SD DHEA-S claws concentration significantly (p<0.01) decreased from birth (210±152.00 pg/mg) to 30 days (91±72.63 pg/mg), evidencing the higher fetal DHEA-S secretion in the last fetal stage of pregnancy in comparison to the first postnatal month of age. According to the type of parturition, higher (p<0.001) DHEA-S claws concentrations were found at birth in puppies born by spontaneous than caesarean parturition (300±167.05 vs 154±112.23 pg/mg, respectively); this finding deserves further investigations. No influence of newborn gender was found. Claws DHEA-S values at birth were a bit higher, but with a lower SD, in comparison to data reported for babies 1-3 weeks old [1]. The trend of decrease is in agreement with data reported for cortisol [2] in dead puppies, and suggests, beside the role of cortisol, the important effects of DHEA-S around the time of birth, also in puppies, as reported for babies [1]. [1] Tegethoff et al. Dehydroepindrosterone in nails of infants: a potential biomarker of intrauterine responses to maternal stress. Biological psychology, 87: 414-420, 2011. [2] Veronesi et al. Coat and claws as new matrices for noninvasive long-term cortisol assessment in dogs from birth up to 30 days of age. Theriogenology, 84: 791-796, 2015.
Najdeno v: osebi
Ključne besede: DHEA-S, claw, dog
Objavljeno: 22.08.2017; Ogledov: 1104; Prenosov: 0
.pdf Polno besedilo (4,69 MB)

6.
Hair cortisol concentrations in New Zealand white rabbits subjected to surgery
Tanja Peric, Antonella Comin, Mirco Corazzin, Marta Montillo, Federico Canavese, Marco Stebel, Alberto Prandi, 2018, izvirni znanstveni članek

Opis: The aim of this study was to assess hair cortisol concentrations in New Zealand white rabbits (Oryctolagus cuniculus) that were subjected to relocation and surgery to evaluate HPA-axis activity; in addition, we used this marker of cortisol secretion to evaluate the allostatic load of animals undergoing surgery. After a period of acclimatisation, which lasted 40 days from their arrival at the enclosure, 19 rabbits were subjected to T1–T12 dorsal arthrodesis (RS), 19 were sham-operated (SS), and 19 were non-operated (CON). Hair samples were collected at the time of arrival (ST1) at the animal facility, and seven other sets of hair samples were collected at 40-day intervals from the same area of skin for a period of 240 days as re-shaved hair (anagen phase): immediately before surgery (ST2) and after the surgery (ST3, ST4, ST5, ST6, ST7, and ST8). The transition from the rabbitry to the animal breeding facility led to a significant increase in cortisol concentration (ST2) in all of the groups. At ST3, the RS group presented higher cortisol concentrations than those of the SS group and the CON group. At ST4, the experimental groups showed similar values that remained constant until ST8. The results show that the management of rabbits undergoing surgery should be evaluated very carefully, and hair cortisol concentrations may provide a means of avoiding the dangerous cumulative effects of additional stressors close to surgery.
Najdeno v: osebi
Ključne besede: animal welfare, cortisol, hair, HPA axis, rabbit, surgery
Objavljeno: 09.02.2018; Ogledov: 705; Prenosov: 0
.pdf Polno besedilo (186,99 KB)

7.
Maternal and fetal hair cortisol concentrations in beef cattle at delivery
Alberto Prandi, Marta Montillo, Maria Cristina Veronesi, Monica Probo, Antonella Comin, Tanja Peric, 2018, objavljeni povzetek znanstvenega prispevka na konferenci

Opis: Hypothalamus-pituitary-adrenal (HPA) axis of both mother and fetus, is one of key mechanisms involved in fetal programming. Cortisol, the end-product of this axis, plays a critical role in regulating fetal and new-born metabolism and development. The aim of the study was to evaluate maternal and fetal cortisol concentrations shortly after delivery on hair samples non-invasively collected. The study was carried out on 11 pairs of beef cows and their calves (calves were 12 because of one case of twins) from the same farm and subjected to the same managerial conditions. Hair from new-born calves and from their mothers was collected on the day of birth shaving their withers to the level of the skin. The hair cortisol concentrations (HCC) were determined by RIA as previously described (Peric et al. 2013: J Dairy Sci 96:3023–3027). The results showed that fetal and maternal HCC ranged from 16.11 to 40.43 pg/mg and from 1.88 to 15.73 pg/mg respectively, with fetal and maternal mean ± SE HCC of 23.72 ± 2.21 and 6.40 ± 1.32 pg/mg respectively (p < 0.001). Fetal HCC were on average 3.5 higher than those of mothers but this ratio differed among the pairs. These differences were also found among the twin calves. These results indicate that in beef cows-and-calves pairs, although HCC at delivery were always higher in calves than in cows, the ratio between fetal and maternal HCC differs among pairs, suggesting an individuality in the fetal programming processes that may also rely on genetic and epigenetic factors.
Najdeno v: osebi
Ključne besede: hair, cortisol, cattle, delivery
Objavljeno: 09.04.2019; Ogledov: 273; Prenosov: 0
.pdf Polno besedilo (802,60 KB)

8.
Analysis of 19 Minerals and Cortisol in Red Deer Hair in Two Different Areas of the Stelvio National Park: A Preliminary Study
Marta Montillo, Tanja Peric, 2019, izvirni znanstveni članek

Opis: The aim of the study was to perform an investigation on the concentration of 19 minerals and cortisol in red deer (Cervus elaphus) hair, a matrix that is easy to collect with non-invasive and painless sampling, able to represent an integrative values of long-term substance concentrations, and able to give useful information, also when performed on dead animals, given its extreme stability over time. In the study thirty-five animals were included, coming from two different sides of a valley in the Stelvio National Park, where official water analysis had pointed out elevated concentrations of As in one of the two orographic sides. Hair cortisol concentrations were measured using a RIA(Radio Immuno Assay), while minerals were detected using ICP-MS (Inductively Coupled Plasma- Mass Spectrometry). Results showed a negative relationship between cortisol and some mineral concentrations (Li, Co, As, Cd, Cr and Tl) and significant differences in some mineral concentrations between park areas (Al, Co, Cu, Cd and Ni). As, Cr and cortisol differences approached statistical significance. This preliminary study represents a step forward in the study of wildlife allostatic load and a valid method for applications in wildlife management programs, in environmental studies and in public health programs.
Najdeno v: osebi
Ključne besede: hair, deer, mineral, cortisol
Objavljeno: 27.08.2019; Ogledov: 127; Prenosov: 6
.pdf Polno besedilo (2,10 MB)

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