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Development of a new microchip based ELISA platform for detection of NGAL
Mingqiang Liu, Mladen Franko, Tatjana Radovanović, 2014, objavljeni povzetek znanstvenega prispevka na konferenci

Najdeno v: osebi
Ključne besede: ELISA, NGAL, TLM
Objavljeno: 22.01.2015; Ogledov: 2466; Prenosov: 21
URL Polno besedilo (0,00 KB)

3.
Microfluidic flow injection thermal lens microscopy for high throughput and sensitive analysis of sub-μL samples
Mladen Franko, Sara Malovrh, Mingqiang Liu, 2016, izvirni znanstveni članek

Opis: An analytical method combining microfluidic flow injection analysis (μFIA) with thermal lens microscopy (TLM) was developed for high throughput and sensitive analysis of sub-μL samples. Performance of the μFIA-TLM was validated for detection of hexavalent chromium [Cr(VI)] in water samples. At different sample injection volumes, detection positions and flow rates, influences of the reaction time and the diffusion of Cr-diphenylcarbazone (DPCO) complexes on the μFIA-TLM signal were investigated. Photodegradation of the Cr-DPCO complex was clearly observed when the absorbed photons per Cr-DPCO is above 1600. After optimization of the TLM with respect to rapid flows (up to 10 cm/s), we achieved a limit of detection of 0.6 ng/mL for Cr(VI) in a 50-μm deep channel. Impacts of interfering ions [V(V), Mo(VI), Fe(III)] on the Cr(VI) determination were found to be small. Cr(VI) in real samples from a cement factory were determined and found in good agreement with results of spectrophotometry. This μFIA-TLM shows advantages over its conventional counterpart, such as eliminating additional sample conditioning, reducing over 100 times the sample consumption to sub-μL and over 10 times the time required for one sample injection to a few seconds (up to 20 samples/min). The optimized μFIA-TLM setup can be applied for fast and sensitive analysis of nonfluoresent sub-μL samples in rapidly flowing mediums.
Najdeno v: osebi
Ključne besede: Spektrometrija TLS, mikroskopija TLM, mikrofluidika, krom (VI)
Objavljeno: 01.06.2016; Ogledov: 1961; Prenosov: 130
.pdf Polno besedilo (828,33 KB)

4.
Determination of Trace Cr(VI) with Diphenylcarbazide by μFIA–Thermal Lens Microscopy
Tatyana Gor’kova, Michail Proskurnina, Mingqiang Liu, Mladen Franko, 2016, izvirni znanstveni članek

Opis: The optimum reaction parameters for the interaction of hexavalent chromium [Cr(VI)] with diphenylcarbazide in microfluidic chips (μFIA) with thermal-lens microscopic detection were selected. The characteristic feature of the applied flow scheme is the injection of the reagent into the stream containing the test metal, which enables in-field and real-time monitoring of Cr(VI) simply by flowing the sample continuously through the microchip. The limit of detection of Cr(VI) under the selected conditions (signal generating wavelength, 514.5 nm; excitation power, 100 mW; detection position, 10 cm downstream from the mixing zone of the microchip; flow rate 10 μL min–1; injection volume, 1.4 μL) is 15 ng mL–1 (2.9 × 10–7 mol L–1). The linear range is 40 ng mL–1 – 10 μg mL–1 with a relative standard deviation no higher than 10% in the concentration range 0.1–1 μg mL–1. The online monitoring by this scheme provides the possibility of up to 360 analyses per hour.
Najdeno v: osebi
Ključne besede: Photothermal lensing, thermal-lens microscopic detection, microfluidic applications, μFIA, hexavalent chromium, diphenylcarbazide
Objavljeno: 12.12.2016; Ogledov: 1512; Prenosov: 0
.pdf Polno besedilo (312,62 KB)

5.
Hemichrome Determination by Thermal Lensing with Polyethylene Glycols for Signal Enhancement in Aqueous Solutions
Mikhail Proskurnin, Mingqiang Liu, Mladen Franko, Viktoriya Galimova, 2018, izvirni znanstveni članek

Opis: The thermal lens technique is proposed for the determination of total hemoglobin in the form of reversible hemichrome. The conditions were optimized (concentration of sodium dodecyl sulfate, 2 mM) to attain the maximum sensitivity with the use of polyethylene glycols as signal enhancers. For polyethylene glycols with molecular weights 1500–35000 Da in a concentration range of 5–15% w/w (5–25 mM), the influence on thermal lens signal enhancement was estimated. It is shown that the use of 5% w/w polyethylene glycol 2000 provides the maximum increase in the thermal lens enhancement factor (by 40%) in comparison with unmodified aqueous solutions. The detection limit of iron(II) tris(1,10-phenanthrolinate) as a model system is 60 nM. Under these conditions, the thermal lens detection limit of hemichrome is 10 nM, which shows a 15-fold enhancement compared to spectro- photometry. Modification of the medium with polyethylene glycols decreases the limit of detection of hemichrome determination by 15% in comparison with unmodified aqueous solutions due to better reproducibility for the range of concentrations from 0.02 to 0.9 μM.
Najdeno v: osebi
Ključne besede: Hemoglobin, hemikrom, optotermična spektroskopija, etilenglikol, spektrometrija s toplotnimi lečami
Objavljeno: 16.04.2018; Ogledov: 996; Prenosov: 0
.pdf Polno besedilo (722,19 KB)

6.
Thermal Lens Spectrometry: Still a Technique on the Horizon
Mladen Franko, Mingqiang Liu, 2016, objavljeni znanstveni prispevek na konferenci

Opis: In this article the historical development of thermal lens spectrometry (TLS) is briefly reviewed for introduction. In continuation, the emphasis is on the recent progresses of TLS for measurements in ensembled sample cells and in microfluidic flow injection systems. Novel theories, instrumentations and their applications for high sample throughput environmental, chemical and biomedical analysis, particularly in micro space, are presented. Discussions are given on the limitations of present TLS systems, that open new horizons for future progress of this technique, which has already found place among routine techniques for chemical analysis. In the last part, proposals for the future development of TLS toward advanced applications in new research fields are presented.
Najdeno v: osebi
Ključne besede: Thermal lens spectrometry, Microfluidic chip, Chemical analysis, Environmental monitoring, Biomedical assay
Objavljeno: 17.05.2016; Ogledov: 1855; Prenosov: 0
.pdf Polno besedilo (404,46 KB)

7.
Progress in thermal lens spectrometry and its applications in microscale analytical devices
Mladen Franko, Mingqiang Liu, 2014, izvirni znanstveni članek

Najdeno v: osebi
Ključne besede: TLS, spektrometrija, mikroskopija, mikroanalizne metode
Objavljeno: 16.06.2016; Ogledov: 1690; Prenosov: 58
URL Polno besedilo (0,00 KB)

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Thermal lens microscopy
Mingqiang Liu, Mladen Franko, 2014, objavljeni znanstveni prispevek na konferenci

Najdeno v: osebi
Ključne besede: mikroskopija, TLS, karakterizacija, optimizacija
Objavljeno: 16.06.2016; Ogledov: 1536; Prenosov: 56
URL Polno besedilo (0,00 KB)

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