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1.
Biological role of Grapevine fanleaf virus (GFLV) in winegrowing region of Northern Primorska
Anastazija Jež Krebelj, 2015, doktorska disertacija

Opis: Grapevines (Vitis vinifera L.) represent one of the most important crops in the world in terms of both production and economic importance. Grapevines are exposed to many types of abiotic stresses (e.g., drought, flooding, low and high temperature, salinity) and biotic stresses (e.g., viruses, bacteria, phytoplasma, fungal disease) during their life-cycle. Therefore, grapevines elicit the appropriate defence mechanisms. In the first part of this study, we monitored the occurrence of Grapevine fanleaf virus (GFLV) infection, which causes progressive decline of infected grapevines and lowers their yield. Grapevines were also tested for the presence of other viruses important for grapevines: Arabis mosaic virus (ArMV), Grapevine leafroll associated virus (GLRaV)-1, -2, -3, -4, -9, Grapevine virus A (GVA), Grapevine fleck virus (GFkV) in this study; and by Cigoj (2015): Grapevine virus B (GVB), Tomato black ring virus (TBRV), Grapevine chrome mosaic virus (GCMV), Tomato ringspot virus (ToRSV), Raspberry ringspot virus (RpRSV), Strawberry latent ringspot virus (SLRSV), and Tobacco ringspot virus (TRSV). Using ELISA, the presence of the following grapevine viruses were detected: GFLV, (GFkV), (GVA), and Grapevine leafroll associated viruses- 1, -2, -3,. A wide range of GFLV symptoms caused by grapevine fanleaf disease in naturally infected vineyards were observed, including leaf, shoot and cluster malformations and leaf yellowing. GFLV is disseminated by its biological vector X. index, and through vegetative propagation of virus-infected material. The spread of GFLV in the vineyards was investigated here. We constructed a spatio-temporal study of the GFLV titres during the seasons and throughout the grapevine, for its distribution in different grapevine organs through the season. This study shows that young leaves have high virus titres through the whole vegetative period, while mature leaves, tendrils and flower/ berry clusters only have high titres at the beginning of the vegetative period. The seeds retain high virus titres after berry colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with an increase outside and at the beginning of the vegetative period. In flower/ berry clusters, mature leaves and tendrils, the GFLV titres decrease significantly over the vegetative period. Additionally, different GFLV titres were shown in five different cultivars, and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptom appearance and GFLV titres was analysed. Grapevines adapt to abiotic stresses and biotic stresses by the expression of a wide range of stress-responsive genes, which are thought to have key roles in stress tolerance and survival. SWP of the infected grapevines through the season was lower than SWP measured for healthy grapevines. For both seasons, there were significant differences in SWP measurements between healthy and GFLV-infected grapevines of ‘Schioppettino’ trained using the single Guyot training system. SWP and RHC of the GFLV-infected grapevines were reduced compared to the healthy controls. The water deficit triggered the production of ABA, which induced the expression of the stress-related gene RD22. Additionally, this study shows that the WRKY gene that is involved in the ABA signalling network is regulated by water deficit. Plant defence responses to water stress also included up-regulation of the F3H2 and LDOX genes, which are involved in anthocyanins synthesis. GFLV infection significant impacted upon the expression of genes involves in ABA biosynthesis, as NCED1 and NCED2, and upon two genes involved in the early stages of anthocyanins synthesis, as CHS2 and F3H1. We also showed that the combination of grapevine cultivar, training system, and environmental conditions impacts on gene expression
Najdeno v: ključnih besedah
Povzetek najdenega: ...Grapevines (Vitis vinifera L.) represent one of the most...
Ključne besede: Vitis vinifera L., grapevine, Grapevine fanleaf virus, GFLV, grapevine disease, virus titre, distribution, fluctuation, ELISA, qPCR, ABA, drought, water status, water deficit, SWP, RHC, anthocyanins, gene expression
Objavljeno: 27.07.2015; Ogledov: 2216; Prenosov: 74
.pdf Polno besedilo (4,19 MB)

2.
Vpliv vinogradniških tehnologij na vsebnost metabolitov v grozdju sorte ‘Modra frankinja’
Ana Marija Tomše, 2015, diplomsko delo

Opis: V grozdju sorte ‘Modra frankinja’ (Vitis vinifera L.), vinorodni okoliš Dolenjska, smo leta 2013 preučevali vpliv vinogradniških tehnologij: odstranjevanja listov v predelu grozdja (razlistanja) pred fazo cvetenja in redčenja grozdja v fazi vèraison v primerjavi s kontrolo. Grozdje smo vzorčili teden pred trgatvijo, v času trgatve in teden po tem. V svežem grozdnem soku smo določili vsebnost suhe snovi, skupnih titrabilnih kislin in pH-vrednost. Vsebnost in profil antocianov smo določili v metanolnih ekstraktih grozdnih kožic s pomočjo tekočinske kromatografije visoke ločljivosti. Redčenje grozdja v fazi vèraison je vplivalo na statistično značilno večje povprečne: mase kožic, vsebnosti suhe snovi in pH-vrednosti v primerjavi z razlistanjem pred fazo cvetenja ob času trgatve in teden po tem, medtem ko se kontrola ni statistično značilno razlikovala od redčenja grozdja. Razlike v povprečni masi grozdja in vsebnosti skupnih titrabilnih kislin med tehnologijami niso bile statistično značilne ob nobenem terminu vzorčenja. Povprečna vsebnost skupnih monomernih antocianov v grozdju je bila ob času trgatve največja pri redčenju grozdja v fazi vèraison, vendar razlike med tehnologijami niso bile statistično značilne. Malvidin 3-glukozid je najbolj zastopan antocian pri sorti ‘Modra frankinja’. Lastnost sorte je tudi manjša vsebnost acilatov od kumaratov. Redčenje grozdja v fazi vèraison je najbolj vplivalo na izboljšano kakovost grozdja glede na spremljane parametre.
Najdeno v: ključnih besedah
Povzetek najdenega: ...V grozdju sorte ‘Modra frankinja’ (Vitis vinifera L.), vinorodni okoliš Dolenjska, smo leta 2013...
Ključne besede: ‘Modra frankinja’ (Vitis vinifera L.), odstranjevanje listov pred fazo cvetenja, redčenje grozdja v fazi vèraison, antociani, profil antocianov.
Objavljeno: 15.09.2015; Ogledov: 1846; Prenosov: 66
.pdf Polno besedilo (3,95 MB)

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