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1.
Development and application of new laser methods in environmental and biomedical research
Azamela Madžgalj, 2007, magistrsko delo

Najdeno v: ključnih besedah
Povzetek najdenega: ...pesticidi, bilirubin, krom, spektroskopija, termične leče, spektrofotometrija, ...
Ključne besede: pesticidi, bilirubin, krom, spektroskopija, termične leče, spektrofotometrija
Objavljeno: 15.10.2013; Ogledov: 2484; Prenosov: 170
URL Polno besedilo (0,00 KB)
Gradivo ima več datotek! Več...

2.
Application of high-performance liquid chromatography combined with ultra-sensitive thermal lens spectrometric detection for simultaneous biliverdin and bilirubin assessment at trace levels in human serum
Mitja Martelanc, Lovro Žiberna, Sabina Passamonti, Mladen Franko, 2016, izvirni znanstveni članek

Opis: We present the applicability of a new ultra sensitive analytical method for the simultaneous determination of biliverdin and bilirubin in human serum. The method comprises isocratic reversed-phase(RP) C18 high-performance liquid chromatography (HPLC) and thermal lens spectrometric detection (TLS)based on excitation by a krypton laser emission line at 407 nm. This method enables the separation of IX-α biliverdin and IX-α bilirubin in 11 min. with limit of detection (LOD) and limit of quantitation (LOQ) for biliverdin of 1.2 nM and 3 nM, and 1nM and 2.8 nM for bilirubin,respectively.In addition, a step-gradient elution was set up, by changing the mobile phase composition, in order to further enhance the sensitivity for bilirubin determination with LOD and LOQ of 0.5 nM and 1.5 nM, respectively. In parallel, an isocraticHPLC-DAD method was developed for benchmarking against HPLC-TLS methods. The LOD and LOQ forbiliverdin were 6 nM and 18 nM, and 2.5 nM and 8nM for bilirubin,respectively. Additionally, both isocratic methods were applied for measuring biliverdin and free bilirubin in human serum samples (from 2 male and 2 female healthy donors). Combining isocratic HPLC method with TLS detector was crucial for first ever biliverdin determination in serum together with simultaneous free bilirubin determination. We showed for the first time the concentration ratio of free bilirubin versus unbound biliverdin in human serum samples.
Najdeno v: ključnih besedah
Ključne besede: Bilirubin, biliverdin, serum, HPLC, TLS
Objavljeno: 29.03.2016; Ogledov: 2117; Prenosov: 0
.pdf Polno besedilo (964,28 KB)

3.
Bilirubin is an Endogenous Antioxidant in Human Vascular Endothelial Cells
Lovro Žiberna, Mitja Martelanc, Mladen Franko, Sabina Passamonti, 2016, izvirni znanstveni članek

Opis: Bilirubin is a standard serum biomarker of liver function. Inexplicably, it is inversely correlated with cardiovascular disease risk. Given the role of endothelial dysfunction in originating cardiovascular diseases, direct analysis of bilirubin in the vascular endothelium would shed light on these relationships. Hence, we used high-performance liquid chromatography coupled with thermal lens spectrometric detection and diode array detection for the determination of endogenous cellular IXα-bilirubin. To confirm the isomer IXα-bilirubin, we used ultra-performance liquid chromatography coupled with a high-resolution mass spectrometer using an electrospray ionization source, as well as tandem mass spectrometric detection. We measured bilirubin in both arterial and venous rat endothelium (0.9–1.5 pmol mg−1 protein). In the human endothelial Ea.hy926 cell line, we demonstrated that intracellular bilirubin (3–5 pmol mg−1 protein) could be modulated by either extracellular bilirubin uptake, or by up-regulation of heme oxygenase-1, a cellular enzyme related to endogenous bilirubin synthesis. Moreover, we determined intracellular antioxidant activity by bilirubin, with EC50 = 11.4 ± 0.2 nM, in the range of reported values of free serum bilirubin (8.5–13.1 nM). Biliverdin showed similar antioxidant properties as bilirubin. We infer from these observations that intra-endothelial bilirubin oscillates, and may thus be a dynamic factor of the endothelial function.
Najdeno v: ključnih besedah
Ključne besede: Antioxidants, bilirubin, endothelial cells
Objavljeno: 07.07.2016; Ogledov: 1921; Prenosov: 195
.pdf Polno besedilo (585,86 KB)

4.
TLS Spectrometry & Detection in Liquid Chromatography, FIA and Microfluidic Systems
Mladen Franko, predavanje na tuji univerzi

Opis: This presentation provides theoretical backround for thermal lens spectrometry (TLS) and TLS microscopy (TLM) and describes the basic instrumentation for TLS and TLM measurements. Presented applications of TLS and TLM include detection in HPLC (carotenoids, bilirubin, biliverdin), FIA (alergens, Ag, Ag+, phytoplankton and cyanobacterial pigments, organophospate pesticides) and TLM detection in microfluidic systems (Cr(VI), microcystin, NGAL, study of diffusion proceses)
Najdeno v: ključnih besedah
Povzetek najdenega: ...and TLM include detection in HPLC (carotenoids, bilirubin, biliverdin), FIA (alergens, Ag, Ag+, phytoplankton and...
Ključne besede: Spekrometry TLS, TLM, HPLC, FIA, microfluidics, bilirubin, biliverdin, carotenoids, alergens, mikrocystin, pigments, organophosphate pesticides, NGAL, Ag, Cr(VI), diffusion
Objavljeno: 16.09.2016; Ogledov: 1655; Prenosov: 0
.pdf Polno besedilo (35,40 MB)

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