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Hair cortisol concentrations in New Zealand white rabbits subjected to surgery
Tanja Peric, Antonella Comin, Mirco Corazzin, Marta Montillo, Federico Canavese, Marco Stebel, Alberto Prandi, 2018, izvirni znanstveni članek

Opis: The aim of this study was to assess hair cortisol concentrations in New Zealand white rabbits (Oryctolagus cuniculus) that were subjected to relocation and surgery to evaluate HPA-axis activity; in addition, we used this marker of cortisol secretion to evaluate the allostatic load of animals undergoing surgery. After a period of acclimatisation, which lasted 40 days from their arrival at the enclosure, 19 rabbits were subjected to T1–T12 dorsal arthrodesis (RS), 19 were sham-operated (SS), and 19 were non-operated (CON). Hair samples were collected at the time of arrival (ST1) at the animal facility, and seven other sets of hair samples were collected at 40-day intervals from the same area of skin for a period of 240 days as re-shaved hair (anagen phase): immediately before surgery (ST2) and after the surgery (ST3, ST4, ST5, ST6, ST7, and ST8). The transition from the rabbitry to the animal breeding facility led to a significant increase in cortisol concentration (ST2) in all of the groups. At ST3, the RS group presented higher cortisol concentrations than those of the SS group and the CON group. At ST4, the experimental groups showed similar values that remained constant until ST8. The results show that the management of rabbits undergoing surgery should be evaluated very carefully, and hair cortisol concentrations may provide a means of avoiding the dangerous cumulative effects of additional stressors close to surgery.
Najdeno v: ključnih besedah
Ključne besede: animal welfare, cortisol, hair, HPA axis, rabbit, surgery
Objavljeno: 09.02.2018; Ogledov: 1303; Prenosov: 0
.pdf Polno besedilo (186,99 KB)

A first survey on hair cortisol of an Alpine ibex (Capra ibex ibex) population.
Alberto Prandi, Tanja Peric, Mirco Corazzin, Antonella Comin, Monica Colitti, 2018, izvirni znanstveni članek

Opis: Biometric measurements of horn, body and physiological variables of 35 Alpine ibex, Capra ibex ibex, (14 females and 21 males, age range: 2-15 years) were analysed in relation to gender and area of origin. Moreover, for the first time hair cortisol concentration was evaluated in this species. The ibexes were live-captured in spring in the Maritime Alps Natural Park. All the horn and body morphometric measurements were significantly different between genders and a statistical difference was found between horn and body data considering the area of origin of ibexes, with the exception of horn circumference and height as well of body and hock length. Among the physiological variables, female ibex showed higher heart rate and glycemia, but tended to have lower respiratory rate than male. The mean hair cortisol concentration was 22.40±1.44 pg/mg (±SE). A different distribution was described for the male hair cortisol concentrations compared to that in females. Obtained data indicate that the geographical area where Alpine ibex lives could affect the endurance of an ibex population but further investigations are needed to understand if intra- and inter-specific competition and different environment could be the basis of the HPA axis activation. Hair cortisol evaluation together with biometric values could be an interesting way to obtain information on the adaptation that the animal attempts to reach in relation to environmental factors and pursuing organism homeostasis. This feature could be important in attempting to preserve wildlife biodiversity that also depends from the state of animal welfare.
Najdeno v: ključnih besedah
Ključne besede: allostatic load, Alps, ibex, biometry, hair cortisol
Objavljeno: 06.03.2018; Ogledov: 1623; Prenosov: 0
.pdf Polno besedilo (1,11 MB)

Maternal and fetal hair cortisol concentrations in beef cattle at delivery
Alberto Prandi, Marta Montillo, Maria Cristina Veronesi, Monica Probo, Antonella Comin, Tanja Peric, 2018, objavljeni povzetek znanstvenega prispevka na konferenci

Opis: Hypothalamus-pituitary-adrenal (HPA) axis of both mother and fetus, is one of key mechanisms involved in fetal programming. Cortisol, the end-product of this axis, plays a critical role in regulating fetal and new-born metabolism and development. The aim of the study was to evaluate maternal and fetal cortisol concentrations shortly after delivery on hair samples non-invasively collected. The study was carried out on 11 pairs of beef cows and their calves (calves were 12 because of one case of twins) from the same farm and subjected to the same managerial conditions. Hair from new-born calves and from their mothers was collected on the day of birth shaving their withers to the level of the skin. The hair cortisol concentrations (HCC) were determined by RIA as previously described (Peric et al. 2013: J Dairy Sci 96:3023–3027). The results showed that fetal and maternal HCC ranged from 16.11 to 40.43 pg/mg and from 1.88 to 15.73 pg/mg respectively, with fetal and maternal mean ± SE HCC of 23.72 ± 2.21 and 6.40 ± 1.32 pg/mg respectively (p < 0.001). Fetal HCC were on average 3.5 higher than those of mothers but this ratio differed among the pairs. These differences were also found among the twin calves. These results indicate that in beef cows-and-calves pairs, although HCC at delivery were always higher in calves than in cows, the ratio between fetal and maternal HCC differs among pairs, suggesting an individuality in the fetal programming processes that may also rely on genetic and epigenetic factors.
Najdeno v: ključnih besedah
Ključne besede: hair, cortisol, cattle, delivery
Objavljeno: 09.04.2019; Ogledov: 783; Prenosov: 0
.pdf Polno besedilo (802,60 KB)

Antonella Comin, Tanja Peric, Mara Colzani, Francesco De Vecchi, Cristina Bergamin, Alberto Prandi, 2018, objavljeni povzetek znanstvenega prispevka na konferenci

Opis: The study of cortisol in hair requires the use of a highly sensitive assay. To date, hair cortisol concentrations (HCC) have been evaluated with different analytical methods as Radio Immune Assay (RIA), Enzyme Immunossay (EIA), Enzyme Linked Immunosorbent Assay (ELISA), ChemiLuminescent Immunoassay (CLIA) and High Performance Liquid Chromatography with Mass Spectrometry (HPLC/MS) or Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS). Amplified luminescent proximity homogeneous assay (AlphaLISA) is an ELISA-like, nonradioactive technology first reported in 1994 [1]. This technology allows the quantitative detection of molecules of interest in a light-induced chemiluminescence immunoassay using a microplate without wash step. The aim of the study was to compare RIA with AlphaLISA method and examine the sensitivity in evaluating cortisol concentrations in animal hair of the Cortisol AlphaLISA kit (PerkinElmer, USA), originally suggested for the analysis of buffer and serum samples. The study has been carried out on 4 samples of calf, 4 samples of foal and 8 samples of sheep hair. After washing with isopropanol, two extracts were obtained from each hair sample. One extract has been evaluated for HCC by RIA as previously described [2,3]. The second extract has been reconstituted with the buffer provided by the Cortisol AlphaLISA Kit and HCC evaluated by the use of this commercially available kit. The preliminary results indicate that the Cortisol AlphaLISA Kit is capable to detect cortisol also in hair samples with high sensitivity, with a detection limit of 17 pg/ml. Intra- and inter-assay coefficients of variation (CVs) were 4.0% and 9.0%, respectively. Cortisol concentrations ranged between 1.31 and 16.94 pg/mg, 6.30 and 57.65 pg/mg, and 2.16 and 45.08 in calf, foal and sheep hair, respectively. The HCC obtained by Cortisol AlphaLISA Kit and RIA showed a good correlation (r=0.79, p<0.01). Considering the possibility to use a low amount of extracted sample, its no-washing procedure and the performances showed, we can conclude that the Cortisol AlphaLISA Kit can be considered an excellent tool to evaluate cortisol concentrations also in hair derived from animal species.
Najdeno v: ključnih besedah
Ključne besede: hair, cortisol, analyses, method, AlphaLISA, Amplified luminescent proximity homogeneous assay
Objavljeno: 09.04.2019; Ogledov: 875; Prenosov: 0
.pdf Polno besedilo (4,05 MB)

Gian Nicola Frongia, Tanja Peric, Marco Muzzeddu, Fiammetta Berlinguer, Giovanni Leoni, Valentina Satta, Cristina Bergamin, Marcello Franchini, Antonella Comin, Salvatore Naitana, Alberto Prandi, 2018, objavljeni povzetek znanstvenega prispevka na konferenci

Opis: During their life, birds face several challenges which can threaten the stability of physiological functions. These situations typically trigger a cascade of endocrine secretions involving the hypothalamic–pituitary–adrenal (HPA) axis, and resulting in the release of both cortisol and corticosterone also in birds. The use of a non-invasive approach to collect samples of biological material derived from natural populations represents a greatly combination for an improvement of knowledge avoiding handling animals. Steroid concentrations in feathers are mainly acquired from the capillary around the follicle during the long period of feather grown reflecting their bloodstream concentrations. Accordingly, a chronic HPA axis activation could be assessed using molted feathers cortisol concentrations. Another hormone, dehydroepiandrosterone (DHEA), is secreted by adrenals and it could be assessed in molted feathers. This study was aimed to evaluate the feasibility of using feathers cortisol and DHEA concentrations to provide a retrospective assessment of the activity of the HPA axis in griffon vulture acquired for health monitoring purposes. For this, we introduced two groups of animals with a known health condition: one group composed by griffons that have experienced a long-term physiologically compromised period (PC group; n=8) and the other group composed by griffons physiologically not compromised (CTRL group; n=9). The feathers cortisol and DHEA analyses were carried out using RIA [1,2] adapted for feathers. The results revealed different feather hormone concentrations between the two groups of animals. The feathers cortisol had a median value higher in the PC group than in the CTRL group, with cortisol concentrations showing a central value 1.6 times higher in those animals that suffered a long-term physiological impairment. The PC group showed also (Kruskal- Wallis test) higher feather concentrations of DHEA (P=0.01) than the CTRL group. Pearson’s correlation coefficients were used to examine the associations between cortisol and DHEA, showing no correlation between cortisol and DHEA feather concentrations in the PC group (r=0.18, P=0.34) and a moderate positive correlation in the CTRL group (r=0.51, P=0.011). In conclusion, our study reveals that molted feathers can be an interesting way to evaluate the physiological status of wild animals by using a non-invasive approach. Our analyses reveal that in addition to cortisol also DHEA could be evaluated to better understand the relationships between these hormones and to determine the resilience condition of wild species.
Najdeno v: ključnih besedah
Objavljeno: 09.04.2019; Ogledov: 717; Prenosov: 0
.pdf Polno besedilo (4,05 MB)

Analysis of 19 Minerals and Cortisol in Red Deer Hair in Two Different Areas of the Stelvio National Park: A Preliminary Study
Marta Montillo, Tanja Peric, 2019, izvirni znanstveni članek

Opis: The aim of the study was to perform an investigation on the concentration of 19 minerals and cortisol in red deer (Cervus elaphus) hair, a matrix that is easy to collect with non-invasive and painless sampling, able to represent an integrative values of long-term substance concentrations, and able to give useful information, also when performed on dead animals, given its extreme stability over time. In the study thirty-five animals were included, coming from two different sides of a valley in the Stelvio National Park, where official water analysis had pointed out elevated concentrations of As in one of the two orographic sides. Hair cortisol concentrations were measured using a RIA(Radio Immuno Assay), while minerals were detected using ICP-MS (Inductively Coupled Plasma- Mass Spectrometry). Results showed a negative relationship between cortisol and some mineral concentrations (Li, Co, As, Cd, Cr and Tl) and significant differences in some mineral concentrations between park areas (Al, Co, Cu, Cd and Ni). As, Cr and cortisol differences approached statistical significance. This preliminary study represents a step forward in the study of wildlife allostatic load and a valid method for applications in wildlife management programs, in environmental studies and in public health programs.
Najdeno v: ključnih besedah
Ključne besede: hair, deer, mineral, cortisol
Objavljeno: 27.08.2019; Ogledov: 571; Prenosov: 30
.pdf Polno besedilo (2,10 MB)

Effects of alfaxalone or propofol on giant-breed dog neonates viability during elective caesarean sections
Monica Melandri, Tanja Peric, 2019, izvirni znanstveni članek

Opis: Attention must be paid to C-section anesthesia effects on mother and offspring. Alfaxalone induction results in improved puppy viability when compared to propofol. The present study aims to evaluate effects of alfaxalone or propofol induction for elective C-section on newborns, expressed as Apgar score and fetal fluids cortisol concentration. Anesthesia was induced with alfaxalone 3 mg/kg iv in 5 bitches (group A), and propofol 4 mg/kg iv in another 5 (group P), maintained with isoflurane. Amniotic and allantoic fluids were collected to determine cortisol concentration. Apgar score, litter size, newborn gender, birth-weight, maternal age, and parity were recorded. ANOVA, U Mann-Whitney test and ANCOVA assessed the effects of drugs on the Apgar score and fetal fluids cortisol. Thirty-six puppies were randomly selected for the study: 16 from group A and 20 from group P. Only the Apgar score significantly differed between groups. ANCOVA confirmed a significantly higher Apgar score in group A underlining the influence of fetal fluids cortisol concentrations, both resulting in covariates. Present results confirm the effect of anesthesia on the Apgar score of newborns, which is significantly higher for alfaxalone than propofol, suggesting the use of fetal fluids cortisol as a covariate. These findings could be a starting point for further investigations when less viable puppies are detected or expected, such as during an emergency C-section. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
Najdeno v: ključnih besedah
Povzetek najdenega: ...expressed as Apgar score and fetal fluids cortisol concentration. Anesthesia was induced with alfaxalone 3...
Ključne besede: Alfaxalone, Apgar score, C-section, Canine neonates, Cortisol, Fetal fluids
Objavljeno: 26.11.2019; Ogledov: 525; Prenosov: 27
.pdf Polno besedilo (234,45 KB)

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