Repozitorij Univerze v Novi Gorici

Iskanje po repozitoriju
A+ | A- | Pomoč | SLO | ENG

Iskalni niz: išči po
išči po
išči po
išči po
* po starem in bolonjskem študiju

Opcije:
  Ponastavi


1 - 2 / 2
Na začetekNa prejšnjo stran1Na naslednjo stranNa konec
1.
Comparison of ex vivo and in vitro human fibroblast ageing models.
Pascal Sommer, Roberta Tiozzo, Giulia Annovi, Federica Boraldi, Daniela Quaglino, 2010, izvirni znanstveni članek

Opis: Several studies have analyzed modulation of gene expression during physiological ageing with interesting, but often contradictory results, depending on the model used. In the present report we compare age-related metabolic and synthetic parameters in human dermal fibroblasts (HDF) isolated from young and old subjects (ex vivo ageing model) and cultured from early up to late cumulative population doublings (CPD) (in vitro ageing model) in order to distinguish changes induced in vivo by the aged environment and maintained in vitro, from those associated with cell senescence and progressive CPD. Results demonstrate that fibroblasts from aged donors, already at early CPD, exhibit an impaired redox balance, highlighting the importance of this parameter during ageing, even in the presence of standard environmental conditions, which are considered optimal for cell growth. By contrast, several proteins, as those related to heat shock response, or involved in endoplasmic reticulum and membrane trafficking, appeared differentially expressed only during in vitro ageing, suggesting that, at high CPD, the whole cell machinery becomes permanently altered. Finally, given the importance of the elastic component for a long-lasting connective tissue structural and functional compliance, this study focuses also on elastin and fibulin-5 synthesis and deposition, demonstrating a close relationship between fibulin-5 and ageing.
Najdeno v: ključnih besedah
Ključne besede: Ageing Fibroblast Connective tissue Oxidative stress Protein expression Elastin
Objavljeno: 23.08.2019; Ogledov: 186; Prenosov: 0
.pdf Polno besedilo (830,45 KB)

2.
Metabolic dysregulation in vitamin E and carnitine shuttle energy mechanisms associate with human frailty
James Nazroo, Royston Goodacre, Neil Pendleton, Frederick Wu, Iain White, Bram Vanhoutte, Gindo Tampubolon, Alan Marshall, Krisztina Mekli, Zahra Rattray, Tarani Chandola, Caroline Johnson, Drupad Trivedi, Yun Xu, Nicholas Rattray, 2019, izvirni znanstveni članek

Opis: Global ageing poses a substantial economic burden on health and social care costs. Enabling a greater proportion of older people to stay healthy for longer is key to the future sustainability of health, social and economic policy. Frailty and associated decrease in resilience plays a central role in poor health in later life. In this study, we present a population level assessment of the metabolic phenotype associated with frailty. Analysis of serum from 1191 older individuals (aged between 56 and 84 years old) and subsequent longitudinal validation (on 786 subjects) was carried out using liquid and gas chromatography-mass spectrometry metabolomics and stratified across a frailty index designed to quantitatively summarize vulnerability. Through multivariate regression and network modelling and mROC modeling we identified 12 significant metabolites (including three tocotrienols and six carnitines) that differentiate frail and non-frail phenotypes. Our study provides evidence that the dysregulation of carnitine shuttle and vitamin E pathways play a role in the risk of frailty.
Najdeno v: ključnih besedah
Povzetek najdenega: ...Global ageing poses a substantial economic burden on health...
Ključne besede: metabolomics, frailty, ageing, LC-MS, serum
Objavljeno: 08.11.2019; Ogledov: 112; Prenosov: 2
.pdf Polno besedilo (2,35 MB)

Iskanje izvedeno v 0 sek.
Na vrh