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José Manuel Carita Gonçalves, 2018, doktorska disertacija

Opis: The available classical diagnostic methods, due to many disadvantages, do not allow effective detection of pathogenic enteric viruses in environmental samples. Due to low concentrations of pathogenic viruses in the sea, it is important to develop an effective concentration procedure for their successful detection. In the first part of the doctoral thesis, we focused primarily on the development of a protocol for an effective concentration of pathogenic enteric viruses in coastal water samples. Monolithic chromatographic columns (BIAseparations) were used for the concentration of rotaviruses and noroviruses, prior to the detection with reverse transcription quantitative PCR in real time (RT-qPCR). We tested the efficiency of concentration using columns of various chemical properties and selected pathogenic enteric viruses (rotavirus and norovirus). Among them, hydrophobic interaction monolithic column (CIM® C4) was the most effective. CIM C4 was used to optimize the concentration step and tested in waters with different salinities. The presence of concentrated viruses was confirmed by RT-qPCR and transmission electron microscope. We have developed a protocol that enables rapid concentration of viruses in coastal waters of various salinities and can be used on-site. The presence of RoV and NoV was surveyed, using the developed concentration protocol, prior to one-step RT-qPCR molecular detection, in the inner part of the Bay of Koper, in mussel farming areas and a swimming area. Rotaviruses, noroviruses and fecal indicator bacteria were frequently detected in the inner part of the Bay of Koper. Rotaviruses and noroviruses were detected in the studied area, with higher rates close to the outfall of the wastewater treatment plant in the estuary of river Rižana and were also detected in the middle of the Bay of Koper and in areas used for recreation and mussel farming. The results show that water bodies, which are otherwise defined as suitable for bathing or mussel farming, based on the results of fecal indicator bacteria, still contain low concentrations of pathogenic enteric viruses. In addition to human pathogenic enteric viruses and faecal coliforms, changes in abundance of bacteria and virus particles were studied in relation to temperature, salinity, inorganic and organic nutrient concentrations in the organically polluted Rižana estuary. Preliminary results showed spatially and seasonally changes in bacterial and viral particles abundance, and bacterial composition spatially and seasonally. However, seasonality plays a greater role in bacterial dynamics.
Najdeno v: ključnih besedah
Povzetek najdenega: ...viruses in environmental samples. Due to low concentrations of pathogenic viruses in the sea, it...
Ključne besede: Concentration of viruses, Enteric viruses, Rotavirus, Norovirus, Feacal coliforms, Feacal contamination, qPCR, RT-qPCR, Monolithic columns, Sewage, Seasonal dynamics, Concentration, Coastal environment, Gulf of Trieste
Objavljeno: 02.07.2018; Ogledov: 2985; Prenosov: 164
.pdf Polno besedilo (1,95 MB)

TLS-FIA System For Screening of Dissolved Iron in Ocean Water
Gaja Tomsič, Dorota Korte, Arne Bratkič, Mladen Franko, 2018, objavljeni povzetek znanstvenega prispevka na konferenci

Najdeno v: ključnih besedah
Povzetek najdenega: ...flow injection analysis, iron concentration, ocean water, thermal lens spectroscopy...
Ključne besede: flow injection analysis, iron concentration, ocean water, thermal lens spectroscopy
Objavljeno: 16.07.2018; Ogledov: 2528; Prenosov: 0
.pdf Polno besedilo (679,78 KB)

CityFlux perfluorocarbon tracer experiments
Fredrik K Petersson, Damien Martin, Iain R White, Stephen J Henshaw, Graham Nickless, Ian Longley, Carl J Percival, Martin Gallagher, Dudley E. Shallcross, 2010, izvirni znanstveni članek

Opis: In June 2006, two perfluorocarbon tracer experiments were conducted in central Manchester UK as part of the CityFlux campaign. The main aim was to investigate vertical dispersion in an urban area during convective conditions, but dispersion mechanisms within the street network were also studied. Paired receptors were used in most cases where one receptor was located at ground level and one at roof level. One receptor was located on the roof of Portland Tower which is an 80m high building in central Manchester. Source receptor distances in the two experiments varied between 120 and 600 m. The results reveal that maximum concentration was sometimes found at roof level rather than at ground level implying the effectiveness of convective forces on dispersion. The degree of vertical dispersion was found to be dependent on source receptor distance as well as on building height in proximity to the release site. Evidence of flow channelling in a street canyon was also found. Both a Gaussian profile and a street network model were applied and the results show that the urban topography may lead to highly effective flow channelling which therefore may be a very important dispersion mechanism should the right meteorological conditions prevail. The experimental results from this campaign have also been compared with a simple urban dispersion model that was developed during the DAPPLE framework and show good agreement with this. The results presented here are some of the first published regarding vertical dispersion. More tracer experiments are needed in order to further characterise vertical concentration profiles and their dependence on, for instance, atmospheric stability. The impact of urban topography on pollutant dispersion is important to focus on in future tracer experiments in order to improve performance of models as well as for our understanding of the relationship between air quality and public health.
Najdeno v: ključnih besedah
Povzetek najdenega: ...600 m. The results reveal that maximum concentration was sometimes found at roof level rather...
Ključne besede: air quality, atmospheric chemistry, concentration (composition), convective system, dispersion, public health, street canyon, tracer, urban area
Objavljeno: 18.07.2019; Ogledov: 1486; Prenosov: 0
.pdf Polno besedilo (1,07 MB)

Tracer concentration profiles measured in central London as part of the REPARTEE campaign
Damien Martin, K Fredrik Petersson, Iain R White, Stephen H Henshaw, Graham Nickless, Amy Lovelock, Janet F Barlow, Tyrone Dunbar, Curtis R Wood, Dudley E. Shallcross, 2011, izvirni znanstveni članek

Opis: There have been relatively few tracer experiments carried out that have looked at vertical plume spread in urban areas. In this paper we present results from two tracer (cyclic perfluorocarbon) experiments carried out in 2006 and 2007 in central London centred on the BT Tower as part of the REPARTEE (Regent's Park and Tower Environmental Experiment) campaign. The height of the tower gives a unique opportunity to study vertical dispersion profiles and transport times in central London. Vertical gradients are contrasted with the relevant Pasquill stability classes. Estimation of lateral advection and vertical mixing times are made and compared with previous measurements. Data are then compared with a simple operational dispersion model and contrasted with data taken in central London as part of the DAPPLE campaign. This correlates dosage with non-dimensionalised distance from source. Such analyses illustrate the feasibility of the use of these empirical correlations over these prescribed distances in central London.
Najdeno v: ključnih besedah
Ključne besede: advection, concentration (composition), dispersion, tracer, urban atmosphere, vertical mixing, vertical profile
Objavljeno: 18.07.2019; Ogledov: 1568; Prenosov: 0
.pdf Polno besedilo (1,49 MB)

Determination of Iron in Environmental Water Samples by FIA-TLS
Mladen Franko, Arne Bratkič, Dorota Korte, Hanna Budasheva, Leja Goljat, Gaja Tomšič, 2019, izvirni znanstveni članek

Opis: The determination of low concentration of iron in natural waters can be difficult due to the complexity of natural water, but primarily because it requires preconcentration of the sample with solvent extraction. In this work we report on results of thermal lens spectrometry (TLS) coupled to flow injection analysis (FIA) as a highly sensitive FIA-TLS method of iron detection. The concentration of iron redox species was determined using 1,10-phenanthroline (PHN), that forms stable complexes with Fe(II) ions which are characterized by an absorption maximum at 508 nm. The TLS system using a 633 nm probe laser and 530 nm pump laser beam was exploited for on-line detection in flow injection analysis, where a PHN solution was used as the carrier solution for FIA. The concentration of the complexing agent affects the quality of the TLS signal, and the optimal concentration was found at 1 mM PHN. The achieved limits of detection (LODs) for Fe(II) and total iron were 33 nM for Fe(II) and 21 nM for total iron concentration. The method was further validated by determining the linear concentration range, specificity in terms of analytical yield and by determining concentration of iron in a water sample from a local water stream.
Najdeno v: ključnih besedah
Povzetek najdenega: ...The determination of low concentration of iron in natural waters can be...
Ključne besede: Flow injection analysis, iron concentration, thermal lens spectroscopy
Objavljeno: 18.12.2019; Ogledov: 1538; Prenosov: 63
.pdf Polno besedilo (690,39 KB)

Elucidating local pollution and site representativeness at the Jungfraujoch, Switzerland through parallel aerosol measurements at an adjacent mountain ridge
Martin Gysel, Maxime Hervo, Urs Baltensperger, Markus Christian Leuenberger, Martine Collaud Coen, Stéphane Affolter, Günther Wehrle, Griša Močnik, Benjamin Brem, Nicolas Bukowiecki, 2021, izvirni znanstveni članek

Opis: Many long-term air pollution and climate monitoring stations face the issue of increasing anthropogenic activities in their vicinity. Furthermore, the spatial representativeness of the sites is often not entirely understood especially in mountainous terrain with complex topographic features. This study presents a 5-year comparison of parallel aerosol measurements (total particle number concentration and equivalent black carbon mass concentration) at the Jungfraujoch in the Swiss Alps (JFJ, 3580 m a.s.l.), and an adjacent mountain ridge, the Jungfrau East Ridge (JER, 3705 m a.s.l.), in 1000 m air-line distance to the main site. The parallel aerosol measurements reveal characteristic differences in the diurnal variations between the two sites under certain specific meteorological conditions. Our analysis estimates that on 20-40% of the days local activities at the Jungfraujoch have a clear influence on the measured time series of the total aerosol number concentration and the equivalent black carbon mass concentration. This influence is mainly seen in form of strong isolated spikes rather than by an increase in the on-site background concentration. They can thus be flagged during the data quality assurance process and filtered from those measurement parameters available at high time resolution. Removing the spikes from the original time series results in daily mean values for the total aerosol number concentration and equivalent black carbon mass concentration that are 5-10 % lower compared to the original signals. During nighttime with hardly any local pollution sources that cause spikes this percentage decreases towards 0%. The signal baselines at the Jungfraujoch and Jungfrau East Ridge correlate well during more than 50% of the days.
Najdeno v: ključnih besedah
Povzetek najdenega: ...of parallel aerosol measurements (total particle number concentration and equivalent black carbon mass concentration) at...
Ključne besede: aerosol long-term monitoring, equivalent black carbon, aerosol number concentration, spatial variation
Objavljeno: 15.03.2021; Ogledov: 613; Prenosov: 26
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Determination of high-time resolution mineral dust concentration in real-time by optical absorption measurements
Matic Ivančič, Irena Ježek, Martin Rigler, Asta Gregorič, Balint Alfoldy, Rok Podlipec, Luka Drinovec, Griša Močnik, 2021, objavljeni povzetek znanstvenega prispevka na konferenci

Najdeno v: ključnih besedah
Ključne besede: mineral dust, concentration, aerosols, air quality
Objavljeno: 17.11.2021; Ogledov: 202; Prenosov: 1
.pdf Polno besedilo (49,11 MB)
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