Analysis of mechanosensing in human cardiac stem cellsElisa Mazzega
, Eliana Pomarè
, Angela Caragnano
, Sebastian Martewicz
, Nicola Elvassore
, Antonio Paolo Beltrami
, Ugo Livi
, 2015, objavljeni povzetek znanstvenega prispevka na konferenci
Opis: Objectives: We have shown that age and pathology impair the biological properties of stem cells isolated from human hearts (CSC) and functional assays showed differences between CSC isolated from normal (DCSC) and end-stage failing (ECSC) hearts. As alterations of mechanical properties of the myocardium, such as stiffening and increased wall stress, are crucial features of cardiac remodeling, this work addresses the biological effects exerted on CSC by mechanical stimuli. Materials and methods: DCSC and ECSC were cultured under deﬁned conditions to mimic speciﬁc features of the pathologic condition: increased mechanical loading (up to 15%, cyclic at 1 Hz), differential substrate stiffness (ranging from 1 to 231 kPa), differential cell densities. After 24, 48 and 72 h, cells were ﬁxed and stained for analysis of proliferation and subcellular localization of YAP or lysed for RT-PCR analysis.Results: Cyclic stretch was signiﬁcantly associated with both increased proliferation of DCSC (n = 6, p<0.0001) and ECSC (n = 4, p = 0.003), and with a signiﬁcant reduction of nuclear localized YAP (nYAP) as a function of time (p<0.05). However, while signiﬁcant correlation between cell density and decreased nYAP (p = 0.003, r2 = 0.37) characterized ECSC, this was not evident for unstretched DCSC, suggesting a less stringent regulation of contact inhibition in DCSC. These data were further conﬁrmed by seeding cells at differential density. As opposed to what previously shown for epithelial cell lines, DCSC did not reduce nYAP positivity as a function of cell density, when grown in serum containing medium, suggesting that soluble factors present in the serum could maintain the nuclear localization of YAP, independently from the cell density. In line, serum signiﬁcantly increased the nYAP expressing cells in DCSC, while a signiﬁcant positive correlation between cell density and nYAP positivity can be demonstrated in DCSC cultured in serum free medium. RT-PCR for YAP-regulated targets conﬁrmed immuno- ﬂuorescence data. Furthermore, independently from the pathologic status, cyclic stretch was signiﬁcantly associated with a persistent YAP signaling at high cell density. Besides, tension and assembly of cytoskeletal network, induced by increasing substrate stiffness, correlates with nYAP (p<0.05) and YAP transcriptional activation (p<0.05). Conclusions: D- and ECSC differ in their mechanosensing properties. However, in the ﬁrst cell type, nYAP localization is dictated by the combined action of paracrine factors and cytoskeletal tension, thus reducing the contact inhibition effect. This ﬁnding is in line with a more primitive phenotype of SC isolated from normal hearts.
Najdeno v: ključnih besedah
Ključne besede: Stem cells, human cardiac stem cells, mechanosensing, mechanotransduction, heart failure, YAP
Objavljeno: 21.03.2017; Ogledov: 777; Prenosov: 0
Polno besedilo (65,67 KB)
THE INCIDENCE OF NEW ECOSYSTEMS (EE – EMERGING ECOSYSTEMS) IN THE KARST BIOSPHERE RESERVEMariya Perepelytsya
, Ӧzkan Karaçam
, Merisa Kapić
, Nika Feigina
, Barbara Šavli
, Matjaž Reya
, Matej Pogorelc
, Klemen Cotič
, 2017, druge monografije in druga zaključena dela
Opis: The need of wood for building and heating, the extensive grazing and population growth in the 18th and 19th centuries led to turning the Karst area in Slovenia into a bare land. In the 19th century organised reforestation started which was successfully performed with Black pine (Pinus nigra J. F. Arnold). Later on, the reforestation was replaced by the process of spontaneous afforestation. The successfulness of reforestation with Black pine also made it possible for the native tree species to grow. The abandoning of the use of agricultural land also contributes to the spontaneous afforestation. In this project we determined the changes in forest cover through time. We used aerial photographs from the years 1957, 1975, 1985 and 2015. By drawing polygons on the maps in the QGIS programme on the areas that are covered with forests, are overgrown or are used for agricultural purpose we compared the areas from the maps of different years. We found out that the area covered with forest had increased by more than 50 % from the year 1957 to 2015. The increase in the area of forest cover due to afforestation provides new habitats for fauna and flora to live in.
Najdeno v: ključnih besedah
Povzetek najdenega: ...Karst, afforestation, new habitat, Black pine, human impact...
Ključne besede: Karst, afforestation, new habitat, Black pine, human impact
Objavljeno: 07.11.2017; Ogledov: 700; Prenosov: 0
Polno besedilo (1,80 MB)
ACCESSIBILITY AS A NON-PREJUDICIAL APPROACH: IS CULTURE BACK TO NORMALITY?Ilaria Bollati
, 2018, doktorska disertacija
Opis: This research explores the general and actual accessibility to cultural spaces and organizations, focusing on the network of relationships between contents, innovation, and participation. It investigates how Culture can be perceived as a normal experience, actually able to set a rich dialogue with each of us, normal ordinary people/consumers. Normality means inclusion and sharing. The proposed investigation is based on a triad of interactions among culture, economics, and design.
Assuming that Culture generates its value from a cognitive approach, or from a dynamic and context-dependent value chain that is subject to a cognitive evolution, this research acknowledges that the cultural experience is subject to a double issue of access:
- The horizontal question is related to complications associated with the structure. Believing that ‘culture is special’ implies the risk of progressively widening the gap between cultural supply and society. Culture is ‘locked’ in specific sites and a big slice of the world’s adult population has yet to be involved in any cultural experience.
- Once physically inside the cultural structure, the vertical issue is generated by the difficulty in entering in contact with the offered contents. This research focuses only on museums and multimedia exhibitions in which the learning process has changed: the research presents itself as a conversation where both ‘those who know’ and ‘those who learn’ play equally active parts in a relative process of understanding.
Starting from the existing processes, forms, previous studies and case studies, the survey yearns for their systematization in innovative models. The process, from theory to practice and vice versa, goes beyond a traditional mechanism of deduction: it moves from specific contexts to the abstraction of replicable approaches.
The question of how the narration emerges guides you toward a new method of analysis, study, and cataloguing; a schematization capable of investigating not only knowledge, but also the visitor's ‘cognitive metabolism’ (how knowledge is acquired) during the multimedia experience; an innovative multipurpose tool, useful for both the museum institution and the designer.
The research finally assesses and takes into account an actual experiment, the outcomes of which may prove to be useful in feeding theoretical implications with empirical experiences: RovelloDue - Piccolo Spazio Politecnico, a temporary multimedia space.
Najdeno v: ključnih besedah
Povzetek najdenega: ...audience development, cognitive accessibility, cultural addiction, culture, human, emotion, immersion, innovation, interactive exhibitions, participation, natural...
Ključne besede: audience development, cognitive accessibility, cultural addiction, culture, human, emotion, immersion, innovation, interactive exhibitions, participation, natural interfaces, normality, technologies
Objavljeno: 13.12.2018; Ogledov: 59; Prenosov: 3
Polno besedilo (45,89 MB)