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1.
Nanobody technology: principles & applications
Ario De Marco, predavanje na tuji univerzi

Opis: Antibodies possess unattainable capacity to bind selectively and at high affinity their cognates. For this reason they have been largely used in applications which rely on specific molecular recognition. Biosensors, nanoparticles, and even cells can be functionalized with antibodies for improving sensitivity and target specificity. However, conventional antibodies (IgGs) are large molecules (150 kDa) that are difficult to engineer. In the last years, antibody fragments have become more and more popular as an effective alternative and specifically nanobodies raised enthusiasm because of their minimal mass (14 kDa), high stability, relative similarity to human sequences, and simplified mutagenesis. Pre-immune nanobody libraries have the further advantage of enabling blind selection for antigens that can be used to discriminate between subpopulations of cells and vesicles. The panning can be performed directly on intact cells and the resulting binders are specific for the native antigen conformation
Najdeno v: ključnih besedah
Povzetek najdenega: ...Nanobodies, in vitro panning, cancer biomarkers, recombinant antibody engineering...
Ključne besede: Nanobodies, in vitro panning, cancer biomarkers, recombinant antibody engineering
Objavljeno: 03.05.2017; Ogledov: 1741; Prenosov: 0
.pdf Polno besedilo (37,92 KB)

2.
Pre-immune nanobody collections and their biotechnological applications
Ario De Marco, predavanje na tuji univerzi

Najdeno v: ključnih besedah
Povzetek najdenega: ...phage libraries, nanobodies, in vitro antibody panning...
Ključne besede: phage libraries, nanobodies, in vitro antibody panning
Objavljeno: 17.09.2018; Ogledov: 392; Prenosov: 0
.pdf Polno besedilo (54,45 KB)

3.
In vitro and in silico approaches to obtain tailored reagents – Nanobodies as model
Ario De Marco, predavanje na tuji univerzi

Najdeno v: ključnih besedah
Povzetek najdenega: ...Immunoreagents, nanobodies, in vitro panning, recombinant antibody production...
Ključne besede: Immunoreagents, nanobodies, in vitro panning, recombinant antibody production
Objavljeno: 21.09.2018; Ogledov: 411; Prenosov: 0
.pdf Polno besedilo (979,15 KB)

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