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11.
Biotechnological applications of nanobody pre-immune libraries
Ario De Marco, predavanje na tuji univerzi

Najdeno v: ključnih besedah
Povzetek najdenega: ... nanobodies, pre-immune libraries, in vitro selection, biomarkers, recombinant...
Ključne besede: nanobodies, pre-immune libraries, in vitro selection, biomarkers, recombinant proteins
Objavljeno: 16.05.2017; Ogledov: 1031; Prenosov: 0
.pdf Polno besedilo (331,92 KB)

12.
Nanobodies as biotechnological tools
Ario de Marco, predavanje na tuji univerzi

Najdeno v: ključnih besedah
Ključne besede: nanobodies, pre-immune phage library, recombinant expression, biomarkers, tumors
Objavljeno: 17.05.2017; Ogledov: 1059; Prenosov: 0
.pdf Polno besedilo (290,82 KB)

13.
Quantification of Circulating Cancer Biomarkers via Sensitive Topographic Measurements on Single Binder Nanoarrays
Elena Ambrosetti, Pamela Paoletti, Alessandro Bosco, Pietro Parisse, Denis Scaini, Elda Tagliabue, Ario de Marco, Loredana Casalis, 2017, izvirni znanstveni članek

Najdeno v: ključnih besedah
Povzetek najdenega: ...Biosensors, immuno-capture, nanobodies, HER2, cancer diagostics...
Ključne besede: Biosensors, immuno-capture, nanobodies, HER2, cancer diagostics
Objavljeno: 22.06.2017; Ogledov: 1048; Prenosov: 76
.pdf Polno besedilo (4,49 MB)

14.
Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitors
Ronan Crepin, Gianluca Veggiani, Selma Djender, Anne Beugnet, Francois Planeix, Christophe Pichon, Sandrine Moutel, Sebastian Amigorena, Franck Pérez, Nicolae Ghinea, Ario De Marco, 2017, izvirni znanstveni članek

Opis: Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to identify useful functional antibodies for further members of the GPCR class.
Najdeno v: ključnih besedah
Ključne besede: nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library
Objavljeno: 19.10.2017; Ogledov: 892; Prenosov: 0
.pdf Polno besedilo (982,69 KB)

15.
Engineered cross-reacting nanobodies simplify comparative oncology between humans and dogs
Elisa Mazzega, Ario De Marco, 2017, izvirni znanstveni članek

Najdeno v: ključnih besedah
Ključne besede: HER2, nanobodies, inter-species cross-reactivity, thermophoresis
Objavljeno: 19.10.2017; Ogledov: 1119; Prenosov: 0
.pdf Polno besedilo (1,58 MB)

16.
In vitro isolation of anti Alexandrium minutum nanobodies from a pre-immune library
Ario De Marco, Marina Cabrini, Alfred Beran, Elisa Mazzega, 2017, objavljeni povzetek znanstvenega prispevka na konferenci

Najdeno v: ključnih besedah
Ključne besede: nanobodies, toxic microalgae, biopanning
Objavljeno: 04.12.2017; Ogledov: 644; Prenosov: 7
.pdf Polno besedilo (61,76 KB)
Gradivo ima več datotek! Več...

17.
Isolation of anti‑extra‑cellular vesicle single‑domain antibodies by direct panning on vesicle‑enriched fractions
Milica Popovic, Elisa Mazzega, Barbara Toffoletto, Ario De Marco, 2018, izvirni znanstveni članek

Najdeno v: ključnih besedah
Povzetek najdenega: ...exosomes, nanobodies, tumor cells...
Ključne besede: exosomes, nanobodies, tumor cells
Objavljeno: 15.01.2018; Ogledov: 786; Prenosov: 59
.pdf Polno besedilo (2,98 MB)

18.
Binding affinity prediction of nanobody–protein complexes by scoring of molecular dynamics trajectories
Miguel Soler, Sara Fortuna, Ario de Marco, Alessandro Laio, 2018, izvirni znanstveni članek

Najdeno v: ključnih besedah
Povzetek najdenega: ...in silico modeling, nanobodies, binding affinity prediction...
Ključne besede: in silico modeling, nanobodies, binding affinity prediction
Objavljeno: 15.01.2018; Ogledov: 858; Prenosov: 0
.pdf Polno besedilo (2,45 MB)

19.
Nanobodies: flexible structures for biotechnological applications
Ario De Marco, vabljeno predavanje na konferenci brez natisa

Najdeno v: ključnih besedah
Ključne besede: nanobodies, in vitro panning, extracellular vesicles, oncology, toxic algae
Objavljeno: 16.03.2018; Ogledov: 740; Prenosov: 0
.pdf Polno besedilo (436,52 KB)

20.
Pre-immune nanobody collections and their biotechnological applications
Ario De Marco, predavanje na tuji univerzi

Najdeno v: ključnih besedah
Povzetek najdenega: ...phage libraries, nanobodies, in vitro antibody panning...
Ključne besede: phage libraries, nanobodies, in vitro antibody panning
Objavljeno: 17.09.2018; Ogledov: 362; Prenosov: 0
.pdf Polno besedilo (54,45 KB)

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