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Opis: The talk will address three closely related topics concerning the verb in Slavic: the structure of the verbal domain, stress patterns and root allomorphy. We focus on data from Slovenian.
Ključne besede: verbs, Slovenian, Distributed Morphology, allomorphy, stress, theme vowels
Objavljeno v RUNG: 13.10.2020; Ogledov: 2545; Prenosov: 0
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Opis: In this talk, we focus on verbs and argue that verbs lack prosodic specification in their lexical entry. We propose that the two different stress patterns in verbs are a consequence of two positions for theme vowels in the verbal domain. Assuming that Slovenian prosody places stress at the final syllable of the deepest cycle (Simonović under review), we argue that the verb forms that surface with a stressed theme vowel (e.g. godrnj-á-mo) have the theme vowel positioned just below the first cyclic head, whereas the remaining verbs have their theme vowel above this position which leads to stem-final stress (vijúg-a-mo).
Ključne besede: verbs, stress, root allomorphy, Slovenian, Distributed Morphology
Objavljeno v RUNG: 07.09.2020; Ogledov: 2575; Prenosov: 0
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Opis: The potential of Arundo donax to grow in degraded soils, characterized by excess of salinity (Na+), and phosphorus deficiency (-P) or excess (+P) also coupled with salinity (+NaP), was investigated by combining in vivo plant phenotyping, quantification of metabolites and ultrastructural imaging of leaves with a transcriptome-wide screening. Photosynthesis and growth were impaired by+Na, -P and+NaP. While+Na caused stomatal closure, enhanced biosynthesis of carotenoids, sucrose and isoprene and impaired anatomy of cell walls, +P negatively affected starch production and isoprene emission, and damaged chloroplasts. Finally, +NaP largely inhibited photosynthesis due to stomatal limitations, increased sugar content, induced/repressed a number of genes 10 time higher with respect to+P and+Na, and caused appearance of numerous and large plastoglobules and starch granules in chloroplasts. Our results show that A. donax is sensitive to unbalances of soil ion content, despite activation of defensive mechanisms that enhance plant resilience, growth and biomass production of A. donax under these conditions.
Ključne besede: Abiotic stress Giant reed Isoprene emission Phosphorus Salinity Transcriptome
Objavljeno v RUNG: 20.04.2020; Ogledov: 2724; Prenosov: 0
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Opis: Arundo donax L. is an invasive grass species with high tolerance to a wide range of environmental stresses. The response of potted A. donax plants to soil stress characterized by prolonged exposure (43 days) to salinity (+Na), to high concentration of phosphorus (+P), and to the combination of high Na and P (+NaP) followed by 14 days of recovery under optimal nutrient solution, was investigated along the entire time-course of the experiment. After an exposure of 43 days, salinity induced a progressive decline in stomatal conductance that hampered A. donax growth through diffusional limitations to photosynthesis and, when combined with high P, reduced the electron transport rate. Isoprene emission from A. donax leaves was stimulated as Na+ concentration raised in leaves. Prolonged growth in P-enriched substrate did not significantly affect A. donax performance, but decreased isoprene emission from leaves. Prolonged exposure of A. donax to + NaP increased the leaf level of H2O2, stimulated the production of carbohydrates, phenylpropanoids, zeaxanthin and increased the de-epoxidation state of the xanthophylls. This might have resulted in a higher stress tolerance that allowed a fast and full recovery following stress relief. Moreover, the high amount of ABA-glucose ester accumulated in leaves of A. donax exposed to + NaP might have favored stomata re-opening further sustaining the observed prompt recovery of photosynthesis. Therefore, prolonged exposure to high P exacerbated the negative effects of salt stress in A. donax plants photosynthetic performances, but enhanced activation of physiological mechanisms that allowed a prompt and full recovery after stress.
Ključne besede: Arundo donax Phosphorus Salinity Stress tolerance Biomass production
Objavljeno v RUNG: 20.04.2020; Ogledov: 2897; Prenosov: 0
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Opis: Several studies have analyzed modulation of gene expression during physiological ageing with interesting, but often contradictory results, depending on the model used. In the present report we compare age-related metabolic and synthetic parameters in human dermal fibroblasts (HDF) isolated from young and old subjects (ex vivo ageing model) and cultured from early up to late cumulative population doublings (CPD) (in vitro ageing model) in order to distinguish changes induced in vivo by the aged environment and maintained in vitro, from those associated with cell senescence and progressive CPD. Results demonstrate that fibroblasts from aged donors, already at early CPD, exhibit an impaired redox balance, highlighting the importance of this parameter during ageing, even in the presence of standard environmental conditions, which are considered optimal for cell growth. By contrast, several proteins, as those related to heat shock response, or involved in endoplasmic reticulum and membrane trafficking, appeared differentially expressed only during in vitro ageing, suggesting that, at high CPD, the whole cell machinery becomes permanently altered. Finally, given the importance of the elastic component for a long-lasting connective tissue structural and functional compliance, this study focuses also on elastin and fibulin-5 synthesis and deposition, demonstrating a close relationship between fibulin-5 and ageing.
Ključne besede: Ageing Fibroblast Connective tissue Oxidative stress Protein expression Elastin
Objavljeno v RUNG: 23.08.2019; Ogledov: 3171; Prenosov: 0
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