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4. Fibroblast involvement in soft connective tissue calcificationPaolo Cianciulli, Giulia Annovi, Federica Boraldi, Ivonne Pasquali Ronchetti, Daniela Quaglino, 2013, pregledni znanstveni članek Opis: Soft connective tissue calcification is not a passive process, but the consequence of metabolic changes of local mesenchymal cells that, depending on both genetic and environmental factors, alter the balance between pro- and anti-calcifying pathways. While the role of smooth muscle cells and pericytes in ectopic calcifications has been widely investigated, the involvement of fibroblasts is still elusive. Fibroblasts isolated from the dermis of pseudoxanthoma elasticum (PXE) patients and of patients exhibiting PXE-like clinical and histopathological findings offer an attractive model to investigate the mechanisms leading to the precipitation of mineral deposits within elastic fibers and to explore the influence of the genetic background and of the extracellular environment on fibroblast-associated calcifications, thus improving the knowledge on the role of mesenchymal cells on pathologic mineralization.
Ključne besede: fibroblasts, PXE, PXE-likedisorders, elastin, extracellularmatrix, ectopiccalcification, mesenchymal
stromalcells
Objavljeno: 23.08.2019; Ogledov: 0; Prenosov: 0
 Polno besedilo (1,31 MB) |
5. Matrix Gla Protein and Alkaline Phosphatase Are Differently Modulated in Human Dermal Fibroblasts from PXE Patients and ControlsDeanna Guerra, Roberta Tiozzo, Tommaso Trenti, Leon J. Schurgers, Cees Vermeer, Giulia Annovi, Federica Boraldi, Daniela Quaglino, 2013, izvirni znanstveni članek Ključne besede: Fibroblast, Vitamin K, Elastin calcification, Proteomics
Objavljeno: 23.08.2019; Ogledov: 0; Prenosov: 0
Polno besedilo (3,86 MB) |
6. Ectopic calcification in β-thalassemia patients is associated with increased oxidative stress and lower MGP carboxylationPaolo Cianciulli, Cees Vermeer, Leon J. Schurgers, Giulia Annovi, Chiara Paolinelli DeVincenzi, Maria Immaculada Garcia Fernandez, Federica Boraldi, Ivonne Pasquali Ronchetti, Daniela Quaglino, 2013, izvirni znanstveni članek Ključne besede: Elastin
Oxidative stress
Matrix Gla protein
Ectopic calcification
Beta-thalassemia
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Polno besedilo (1,30 MB) |
7. Heparan Sulfate Affects Elastin Deposition in Fibroblasts Cultured from Donors of Different AgesBruna Parma, Roberta Tiozzo, Deanna Guerra, Pasquale Moscarelli, Federica Boraldi, Giulia Annovi, Pascal Sommer, Daniela Quaglino, 2012, izvirni znanstveni članek Opis: Heparan sulfate (HS), due to its presence on the cell surface and in the extracellular milieu and its ability to
modulate cell signaling, has a fundamental role in both physiological and pathological conditions. For decades
we have demonstrated the occurrence of interactions between glycosaminoglycans (GAGs) and elastic fibers. In
particular, we have recently shown that HS is present inside elastic fibers and plays a role in the assembly and
stability of elastin coacervates. Elastin represents, within the extracellular matrix, the component most severely
affected during aging, and changes in the synthesis and posttranslational modifications of HS have been described,
possibly influencing cellular behavior and protein interactions. Thus, the present study has investigated,
in two different in vitro experimental models, the role of HS on elastin deposition and assembly. Results
demonstrate that: (1) Biological effects of HS are partly dependent on the physicochemical characteristics of the
GAGs; (2) HS does not affect attachment, viability, and growth of human dermal fibroblasts; (3) HS does not
modify elastin gene expression nor elastin synthesis, but favors a-elastin aggregation and, independently from
the age of donors, elastin assembly; (4) HS significantly increases the expression of fibulin 5, and these effects are
especially evident in fibroblasts isolated from aging donors. These data provide a better understanding of the
biological role of HS and offer new perspectives regarding the possibility of restoring and/or preserving the
elastic component with aging.
Ključne besede: Heparan sulfate, Elastin, Fibroblasts
Objavljeno: 23.08.2019; Ogledov: 0; Prenosov: 0
Polno besedilo (1,51 MB) |
8. Comparison of ex vivo and in vitro human fibroblast ageing models.Pascal Sommer, Roberta Tiozzo, Giulia Annovi, Federica Boraldi, Daniela Quaglino, 2010, izvirni znanstveni članek Opis: Several studies have analyzed modulation of gene expression during physiological ageing with interesting, but often contradictory results, depending on the model used. In the present report we compare age-related metabolic and synthetic parameters in human dermal fibroblasts (HDF) isolated from young and old subjects (ex vivo ageing model) and cultured from early up to late cumulative population doublings (CPD) (in vitro ageing model) in order to distinguish changes induced in vivo by the aged environment and maintained in vitro, from those associated with cell senescence and progressive CPD. Results demonstrate that fibroblasts from aged donors, already at early CPD, exhibit an impaired redox balance, highlighting the importance of this parameter during ageing, even in the presence of standard environmental conditions, which are considered optimal for cell growth. By contrast, several proteins, as those related to heat shock response, or involved in endoplasmic reticulum and membrane trafficking, appeared differentially expressed only during in vitro ageing, suggesting that, at high CPD, the whole cell machinery becomes permanently altered. Finally, given the importance of the elastic component for a long-lasting connective tissue structural and functional compliance, this study focuses also on elastin and fibulin-5 synthesis and deposition, demonstrating a close relationship between fibulin-5 and ageing.
Ključne besede: Ageing
Fibroblast
Connective tissue
Oxidative stress
Protein expression
Elastin
Objavljeno: 23.08.2019; Ogledov: 0; Prenosov: 0
Polno besedilo (830,45 KB) |
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10. Fibroblast protein profile analysis highlights the role of oxidative stress and vitamin K recycling in the pathogenesis of pseudoxanthoma elasticum.Ivonne Pasquali Ronchetti, Roberta Tiozzo, Giorgio De Santis, Fulvio Panico, Garcia Fernandez Maria Immaculada, Paolinelli Devincenzi Chiara, Deanna Guerra, Giulia Annovi, Federica Boraldi, Daniela Quaglino, 2009, izvirni znanstveni članek Opis: Pseudoxanthoma elasticum (PXE) is a genetic disorder associated to mutations in the ABCC6 gene; however, the pathogenetic mechanisms leading to elastic fibre calcifications and to clinical manifestations are still unknown. Dermal fibroblasts, directly involved in the production of the extracellular milieu, have been isolated from healthy subjects and from patients affected by PXE, cultured in vitro and characterized for their ability to produce reactive oxygen species, for structural and functional properties of their cell membranes, for changes in their protein profile. Data demonstrate that oxidative stress has profound and endurable consequences on PXE fibroblast phenotype being responsible for: reduced levels of global DNA methylation, increased amount of carbonylated proteins and of lipid peroxidation products, altered structural properties of cell membranes, modified protein expression. Data shed new light on the pathogenetic pathways in PXE, by identifying a network of proteins affecting elastic fibre calcification through inefficient vitamin K recycling, and highlight the role of differentially expressed proteins as targets for validating the efficacy of future therapeutic strategies aiming to delay and/or revert the pathologic phenotype of PXE fibroblasts. Moreover, data open new perspectives for investigating PXE-like phenotypes in the absence of ABCC6 mutations.
Ključne besede: Ectopic calcification / Elastin / Fibroblast proteome / MRP6 / PXE
Objavljeno: 23.08.2019; Ogledov: 0; Prenosov: 0
Polno besedilo (650,81 KB) |
