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Vpliv klasične maceracije in maceracije celega grozdja na kemijske in senzorične lastnosti vina Modri pinot
Margareta Lavrenčič, 2019, diplomsko delo

Opis: Maceracija grozdja je ključna tehnološka faza pri pridelavi rdečih vin. Na podlagi znanja, tehnologije in izkušenj ima ta tehnološki postopek velik vpliv na končno kakovost vina. V diplomski nalogi smo preučevali vpliv klasične maceracije in maceracije celega grozdja na kemijske in senzorične lastnosti vina modri pinot. Na podlagi različnih analiz smo določali: prosto žveplo, alkohol, skupne kisline, intenziteto in ton barve, skupne antociane, skupne fenole ter izvedli senzorično analizo. Rezultati našega poskusa kažejo, da maceracija celega grozdja daje vino z manjšo vsebnostjo skupnih kislin v primerjavi s klasično maceracijo. Manjša vsebnost skupnih kislin v vinu pri maceraciji s pecljevino se izraţa tudi z značilno višjim pH-jem vina. Vino maceracije s pecljevino je večji porabnik žvepla, vsebuje bistveno več skupnih fenolov in posledično imamo tudi večjo intenziteto barve, ki pa se pri vinu modri pinot izraža v večji rumeno-rjavih tonov barve. Senzorična analiza je potrdila kemijske analize barve, pokazala, da maceracija celega grozdja v osnovi daje nekoliko manj sadna vina, vendar s poudarjeno noto temnega sadja, zelenih not in začimb. Vino maceracije celega grozdja je bilo boljše sprejeto, saj je imelo prijetnejšo kislino in je bilo taninsko bolj uglajeno.
Ključne besede: Vino, Vitis vinifera L. 'Modri pinot', maceracija, peceljevina, kemijski parametri vina, senzorične lastnosti vina
Objavljeno: 26.07.2019; Ogledov: 147; Prenosov: 2
.pdf Polno besedilo (1,09 MB)

On a class of D-division hypernear-ring
Irina Cristea, Sanja Jančić-Rašović, objavljeni povzetek znanstvenega prispevka na konferenci

Ključne besede: hypernear-ring, distributivity, near-ring
Objavljeno: 25.07.2019; Ogledov: 103; Prenosov: 0
.pdf Polno besedilo (7,38 MB)

Multiendomorphisms as a tool to construct new hyperrings
Sanja Jančić-Rašović, Irina Cristea, 2015, objavljeni povzetek znanstvenega prispevka na konferenci

Ključne besede: hyperring, multiendomorphism
Objavljeno: 25.07.2019; Ogledov: 153; Prenosov: 0
.pdf Polno besedilo (2,79 MB)

Manjšinska literatura in literatura obmejnega območja EDUKA2
ana toroš, druga izvedena dela

Opis: Vodenje projektne delovne skupine EDUKA2 DS 3.1.5. - skupne učne enote o manjšinski literaturi ter literaturi obmejnega območja
Ključne besede: EDUKA2, Trst, Gorica, Videm, Istra, literatura, didaktika
Objavljeno: 23.07.2019; Ogledov: 154; Prenosov: 0
.pdf Polno besedilo (991,88 KB)

Breathomics and its Application for Disease Diagnosis: A Review of Analytical Techniques and Approaches
Enzo A Palombo, Konstantinos A Kouremenos, Ding Y Oh, Iain R White, David J Beale, Avinash V Karpe, Oliver A H Jones, 2018, samostojni znanstveni sestavek ali poglavje v monografski publikaciji

Opis: The application of metabolomics to an ever-greater variety of sample types is a key focus of systems biology research. Recently, there has been a strong focus on applying these approaches toward the rapid analysis of metabolites found in non-invasively acquired samples, such as exhaled breath (also known as ‘breathomics’). The sampling process involved in collecting exhaled breath is nonintrusive and comparatively low-cost. It uses a series of globally approved methods and provides researchers with easy access to the metabolites secreted by the human body. Owing to its accuracy and rapid nature, metabolomic analysis of breath is a rapidly growing field that has proven effective in detecting and diagnosing the early stages of numerous diseases and infections. This review discusses the various collection and analysis methods currently applied in breathomics research. Some of the salient research completed in this field to date is also assessed and discussed in order to provide a basis for possible future scientific directions.
Ključne besede: Metabolomics, breath research, VOCs, breathomics
Objavljeno: 22.07.2019; Ogledov: 119; Prenosov: 0
.pdf Polno besedilo (25,96 MB)

Capturing and Storing Exhaled Breath for Offline Analysis
Stephen J Fowler, Iain R White, 2019, samostojni znanstveni sestavek ali poglavje v monografski publikaciji

Opis: In this chapter we will summarize and discuss methods for the capture and storage of exhaled breath, prior to offline (and indirect online) analysis. We will detail and compare methods currently in use, including their applications, key strengths, and limitations. In synthesizing the best features of each technique, we will propose an ideal standardized breath sampling solution, and give a personal vision on the next steps to be taken in this exciting area of breath research.
Ključne besede: Breath analysis, Breath sampling, Offline analysis, Thermal desorption, Gas chromatography-mass spectrometry
Objavljeno: 22.07.2019; Ogledov: 71; Prenosov: 0
.pdf Polno besedilo (36,24 MB)

Structural and functional determinants of TDP-43 aggregation
Sanja Škaro, 2019, doktorska disertacija

Opis: TDP-43 (TAR DNA-binding protein) is an hnRNP that was identified as the main component of the brain inclusions characteristically found in patients suffering of Amyotrophic Lateral Sclerosis and Frontotemporal Lobar Degeneration. As an hnRNP protein, TDP-43 fulfills diverse roles in mRNA metabolism, localization and transport. Structurally, TDP-43 is composed of a well conserved N terminal domain (NTD), two RRM domains of which RRM1 is necessary for recognizing and binding to its target, UG rich RNA sequences, and the C-terminal domain (CTD) which is a Glycine rich domain. The CTD also contains a Q/N rich region that plays a key role in protein aggregation and interaction with another hnRNP proteins and polyglutamine repeats. This thesis focus on the structural determinants involved in the different TDP-43 interactions with itself and with other hnRNPs. Both the carboxyl and amino terminal domains are involved in these interactions. We have mapped the regions more relevant for the function of TDP-43 and for the aggregation process characteristic of the pathological pathway leading to neurodegeneration. We have started to further study the N-terminal domain. Previous results in our laboratory using a cellular aggregation model have shown that the N-terminal domain is also necessary for sequestering the endogenous TDP-43 into the aggregates. In particular, the intact NTD, specifically residues 1 to 77, have been shown to be needed to efficiently recruit TDP-43 monomers into these aggregates. We have extended our knowledge of NTD structure and function, by assessing the behavior of a series of proteins in which key structural features (α-helix and β-sheets) were modified and TDP-43 splicing function together with structure via NMR were analyzed. It was found that by disrupting protein secondary structure in the NTD (mutation in α-helix NTD-31V/R-32T/R) the capacity of the aggregates to sequester enough TDP-43 to induce loss of function was lost.In fact, this protein is also unable to recovery TDP-43 functionality when it is disrupted due to sequestration of the endogenous TDP-43 in add back experiments. Disturbing protein stability through substitution of residues in α-helix also affects its ability to form an active conformation. On the other hand, synthesis of hybrid peptides containing certain NTD and CTD segments was performed in order to see if they are capable to bind to the TDP-43 aggregates. However, it has been shown that these synthetic peptides have a greater ability to induce TDP-43 aggregation than to bind to them, probably due to specific functional characteristics of NTD and CTD segments used for their synthesis.The main focus of the thesis was on the C-terminal domain sequences involved in protein-protein interaction, misfolding and aggregation.A comparison of human, mouse, zebrafish, Annelida, flatworms and Drosophila showed a very strong conservation of the NTD and RRMs, but the C terminal regions of human and other TDP-43 orthologues are very different.I have studied Human and Drosophila melanogaster orthologues, because Drosophila orthologue contains different paralogs of TDP-43.Through a series of deletions and mutations it was shown that the shorter paralog of Drosophila TDP-43 (TBPH-RA) is more active than the longer one (TBPH-RC), and that this is due to a combination of two factors: 1. TBPH-RC by itself aggregates more than TBPH-RA, 2. The functionality of TBPH-RC is downregulated by intramolecular interactions in the C terminal domain. Apparently there is a cation-π interaction involving Tryptophan and Arginine in TBPH-RC that has a high relevance to the protein function and is lacking in the TBPH-RA.Overall this data has identified structural features essential for the proper function of TDP-43.In addition, we have also identified sequences that are critical in the pathological aggregation process of TDP-43 that lead to the characteristic brain inclusions in ALS and FTLD and to the loss of functionality
Ključne besede: TDP-43 structural determinants, hybrid peptides, protein-protein interactions, intramolecular interaction, cation-π interaction, Drosophila orthologues.
Objavljeno: 22.07.2019; Ogledov: 142; Prenosov: 2
.pdf Polno besedilo (6,84 MB)

The effect of serum withdrawal on the protein profile of quiescent human dermal fibroblasts in primary cell culture.
Quaglino Daniela, Tiozzo Roberta, Paolinelli DeVincenzi Chiara, Annovi Giulia, Boraldi Federica, 2008, izvirni znanstveni članek

Opis: The effect of serum deprivation on proliferating cells is well known, in contrast its role on primary cell cultures, at confluence, has not been deeply investigated. Therefore, in order to explore the response of quiescent cells to serum deprivation, ubiquitous mesenchymal cells, as normal human dermal fibroblasts, were grown, for 48 h after confluence, in the presence or absence of 10% FBS. Fibroblast behaviour (i.e. cell morphology, cell viability, ROS production and elastin synthesis) was evaluated morphologically and biochemically. Moreover, the protein profile was investigated by 2-DE and differentially expressed proteins were identified by MS. Serum withdrawal caused cell shrinkage but did not significantly modify the total cell number. ROS production, as evaluated by the dihydroethidium (DH2) probe, was increased after serum deprivation, whereas elastin synthesis, measured by a colorimetric method, was markedly reduced in the absence of serum. By proteome analysis, 41 proteins appeared to significantly change their expression, the great majority of protein changes were related to the cytoskeleton, the stress response and the glycolytic pathway. Data indicate that human dermal fibroblasts in primary cell culture can adapt themselves to environmental changes, without significantly altering cell viability, at least after a few days of treatment, even though serum withdrawal represents a stress condition capable to increase ROS production, to influence cell metabolism and to interfere with cell behaviour, favouring the expression of several age-related features.
Ključne besede: Dermal fibroblasts / Primary cell culture / ROS production / Serum withdrawal
Objavljeno: 22.07.2019; Ogledov: 136; Prenosov: 0
.pdf Polno besedilo (462,68 KB)

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