3421. 17β-Estradiol and testosterone amniotic concentrations in at term born puppies: preliminary resultsJasmine Fusi, Tanja Peric, 2019, objavljeni povzetek znanstvenega prispevka na konferenci Opis: The study of fetal sexual steroid hormones production during pregnancy deserve scientific interest in both humans and animals, to better understand the normal sexual development, but also in the recently investigated impact on the future health outcomes (Kuijper et al, 2013). Many studies reported the early production of testosterone by the testes during the fetal stage
of development; however, also estrogens were recognized to play a role in developing programming. Because of the scarce knowledge about this topic in the dog, the present study was aimed to assess the 17β-estradiol (E2) and testosterone (T) amniotic concentrations at birth in normal newborn dogs. The study was conducted on 31 normal puppies, 16 males and 15
females, born by 10 healthy bitches belonging to several breeds, submitted to elective Caesarean section at term, with litter-size ranging between 1 to 5. Amniotic fluid from each puppy was collected (Meloni et al, 2014) and immediately stored at - 20 ° C until analysis by RIA. Amniotic E2 concentrations in male and female puppies were 124±66.82 pg/ml and 109±42.15 pg/ml, respectively; while amniotic T concentrations were 124±91.24 pg/ml and 114±71.72 pg/ml, in male and female puppies, respectively, without significant differences between the two genders. These preliminary results showed that both E2 and T were detectable in canine amniotic fluid, but without differences between male and female puppies. The wide standard deviations found deserve further investigations on a larger number of subjects, to identify possible causes, such as the effect played by the bitch.
Kuijper EA, Ket JC, Caanen MR, Lambalk CB. Reproductive hormone concentrations in pregnancy and neonates: a systematic review. Reprod Biomed Online 2013; 27:33-63; Meloni T, Comin A, Rota A, Peric T, Contri A, Veronesi MC. IGF-I and NEFA concentrations in fetal fluids of term pregnancy dogs. Theriogenology 2014;8:1307-11.
Ključne besede: 17β-Estradiol, testosterone, amniotic fluid, fetal fluid, dog, puppy
Objavljeno v RUNG: 29.08.2019; Ogledov: 3202; Prenosov: 0
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3423. Raman LIDARs and atmospheric calibration along the line-of-sight of the Cherenkov Telescope ArraySamo Stanič, Longlong Wang, Marko Zavrtanik, 2019, objavljeni znanstveni prispevek na konferenci Opis: The Cherenkov Telescope Array (CTA) is the next generation ground-based observatory for
gamma-ray astronomy at very-high energies. Employing more than 100 (north and south sites)
Imaging Atmospheric Cherenkov Telescopes in the northern and southern hemispheres, it was
designed to reach unprecedented sensitivity and energy resolution. Understanding and correcting
for systematic biases on the absolute energy scale and instrument response functions will be a cru-
cial issue for the performance of CTA. The Montpellier group and the Spanish/Italian/Slovenian
collaboration are currently building two Raman LIDAR prototypes for the online atmospheric cal-
ibration along the line-of-sight of the CTA. Requirements for such a solution include the ability
to characterize aerosol extinction at two wavelengths to distances up to 30 km with an accuracy
better than 5%, within exposure time scales of about a minute, steering capabilities and close
interaction with the CTA array control and data acquisition system as well as other auxiliary in-
struments. Our Raman LIDARs have design features that make them different from those used
in atmospheric science and are characterized by large collecting mirrors (∼2.5 m 2 ), liquid light-
guides that collect the light at the focal plane and transport it to the readout system, reduced
acquisition time and highly precise Raman spectrometers. The Raman LIDARs will participate in
a cross-calibration and characterization campaign of the atmosphere at the CTA North site at La
Palma, together with other site characterization instruments. After a one-year test period there, an
in-depth evaluation of the solutions adopted by the two projects will lead to a final Raman LIDAR
design proposal for both CTA sites.
Ključne besede: Raman lidar atmospheric calibration Cherenkov Telescope Array
Objavljeno v RUNG: 29.08.2019; Ogledov: 3436; Prenosov: 102
 Celotno besedilo (1,29 MB) |
3424. Analysis of 19 Minerals and Cortisol in Red Deer Hair in Two Different Areas of the Stelvio National Park: A Preliminary StudyMarta Montillo, Tanja Peric, 2019, izvirni znanstveni članek Opis: The aim of the study was to perform an investigation on the concentration of 19 minerals and
cortisol in red deer (Cervus elaphus) hair, a matrix that is easy to collect with non-invasive and painless
sampling, able to represent an integrative values of long-term substance concentrations, and able to
give useful information, also when performed on dead animals, given its extreme stability over time.
In the study thirty-five animals were included, coming from two different sides of a valley in the Stelvio
National Park, where official water analysis had pointed out elevated concentrations of As in one of the
two orographic sides. Hair cortisol concentrations were measured using a RIA(Radio Immuno Assay),
while minerals were detected using ICP-MS (Inductively Coupled Plasma- Mass Spectrometry).
Results showed a negative relationship between cortisol and some mineral concentrations (Li,
Co, As, Cd, Cr and Tl) and significant differences in some mineral concentrations between park
areas (Al, Co, Cu, Cd and Ni). As, Cr and cortisol differences approached statistical significance.
This preliminary study represents a step forward in the study of wildlife allostatic load and a valid
method for applications in wildlife management programs, in environmental studies and in public
health programs.
Ključne besede: hair, deer, mineral, cortisol
Objavljeno v RUNG: 27.08.2019; Ogledov: 2970; Prenosov: 97
Celotno besedilo (2,10 MB) |
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3428. Fibroblast involvement in soft connective tissue calcificationIvonne Pasquali Ronchetti, Federica Boraldi, Giulia Annovi, Paolo Cianciulli, Daniela Quaglino, 2013, pregledni znanstveni članek Opis: Soft connective tissue calcification is not a passive process, but the consequence of metabolic changes of local mesenchymal cells that, depending on both genetic and environmental factors, alter the balance between pro- and anti-calcifying pathways. While the role of smooth muscle cells and pericytes in ectopic calcifications has been widely investigated, the involvement of fibroblasts is still elusive. Fibroblasts isolated from the dermis of pseudoxanthoma elasticum (PXE) patients and of patients exhibiting PXE-like clinical and histopathological findings offer an attractive model to investigate the mechanisms leading to the precipitation of mineral deposits within elastic fibers and to explore the influence of the genetic background and of the extracellular environment on fibroblast-associated calcifications, thus improving the knowledge on the role of mesenchymal cells on pathologic mineralization.
Ključne besede: fibroblasts, PXE, PXE-likedisorders, elastin, extracellularmatrix, ectopiccalcification, mesenchymal
stromalcells
Objavljeno v RUNG: 23.08.2019; Ogledov: 3154; Prenosov: 0
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3429. Matrix Gla Protein and Alkaline Phosphatase Are Differently Modulated in Human Dermal Fibroblasts from PXE Patients and ControlsFederica Boraldi, Giulia Annovi, Cees Vermeer, Leon J. Schurgers, Tommaso Trenti, Roberta Tiozzo, Deanna Guerra, Daniela Quaglino, 2013, izvirni znanstveni članek Ključne besede: Fibroblast, Vitamin K, Elastin calcification, Proteomics
Objavljeno v RUNG: 23.08.2019; Ogledov: 3379; Prenosov: 0
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3430. Ectopic calcification in β-thalassemia patients is associated with increased oxidative stress and lower MGP carboxylationFederica Boraldi, Maria Immaculada Garcia Fernandez, Chiara Paolinelli DeVincenzi, Giulia Annovi, Leon J. Schurgers, Cees Vermeer, Paolo Cianciulli, Ivonne Pasquali Ronchetti, Daniela Quaglino, 2013, izvirni znanstveni članek Ključne besede: Elastin
Oxidative stress
Matrix Gla protein
Ectopic calcification
Beta-thalassemia
Objavljeno v RUNG: 23.08.2019; Ogledov: 3193; Prenosov: 0
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