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4753. The VPS4 component of the ESCRT machinery plays an essential role in HPV infectious entry and capsid disassemblyJustyna Broniarczyk, David Pim, Paola Massimi, Martina Bergant Marušič, Anna Gozdzicka-Jozefiak, Colin Crump, Lawrence Banks, 2017, izvirni znanstveni članek Opis: Human Papillomavirus (HPV) infection involves multiple steps, from cell attachment, through endocytic trafficking towards the trans-Golgi network, and, ultimately, the entry into the nucleus during mitosis. An essential viral protein in infectious entry is the minor capsid protein L2, which engages different components of the endocytic sorting machinery during this process. The ESCRT machinery is one such component that seems to play an important role in the early stages of infection. Here we have analysed the role of specific ESCRT components in HPV infection, and we find an essential role for VPS4. Loss of VPS4 blocks infection with multiple PV types, suggesting an evolutionarily conserved critical step in infectious entry. Intriguingly, both L1 and L2 can interact with VPS4, and appear to be in complex with VPS4 during the early stages of virus infection. By using cell lines stably expressing a dominant-negative mutant form of VPS4, we also show that loss of VPS4 ATPase activity results in a marked delay in capsid uncoating, resulting in a defect in the endocytic transport of incoming PsVs. These results demonstrate that the ESCRT machinery, and in particular VPS4, plays a critical role in the early stages of PV infection.
Ključne besede: HPV, ESCRT machinery, infection
Objavljeno v RUNG: 08.05.2017; Ogledov: 4493; Prenosov: 272
 Celotno besedilo (1,10 MB) |
4754. Nanobody technology: principles & applicationsArio De Marco, predavanje na tuji univerzi Opis: Antibodies possess unattainable capacity to bind selectively and at high affinity their cognates. For this reason they have been largely used in applications which rely on specific molecular recognition. Biosensors, nanoparticles, and even cells can be functionalized with antibodies for improving sensitivity and target specificity. However, conventional antibodies (IgGs) are large molecules (150 kDa) that are difficult to engineer. In the last years, antibody fragments have become more and more popular as an effective alternative and specifically nanobodies raised enthusiasm because of their minimal mass (14 kDa), high stability, relative similarity to human sequences, and simplified mutagenesis. Pre-immune nanobody libraries have the further advantage of enabling blind selection for antigens that can be used to discriminate between subpopulations of cells and vesicles. The panning can be performed directly on intact cells and the resulting binders are specific for the native antigen conformation
Ključne besede: Nanobodies, in vitro panning, cancer biomarkers, recombinant antibody engineering
Objavljeno v RUNG: 03.05.2017; Ogledov: 4958; Prenosov: 0
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4757. Combined fit of spectrum and composition data as measured by the Pierre Auger ObservatoryA. Aab, Andrej Filipčič, Gašper Kukec Mezek, Ahmed Saleh, Samo Stanič, Marta Trini, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, 2017, izvirni znanstveni članek Ključne besede: kozmični žarki ektremnih energij, medgalaktični prostor, Observatorij Pierre Auger
Objavljeno v RUNG: 21.04.2017; Ogledov: 4131; Prenosov: 0
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4758. Time- and angle-resolved photoemission spectroscopy with optimized high-harmonic pulses using frequency-doubled Ti:Sapphire lasersSteffen Eich, Jurij Urbančič, 2014, izvirni znanstveni članek Opis: Time- and angle-resolved photoemission spectroscopy (trARPES) using femtosecond extreme ultraviolet high harmonics has recently emerged as a powerful tool for investigating ultrafast quasiparticle dynamics in correlated-electron materials. However, the full potential of this approach has not yet been achieved because, to date, high harmonics generated by 800 nm wavelength Ti:Sapphire lasers required a trade-off between photon flux, energy and time resolution. Photoemission spectroscopy requires a quasi-monochromatic output, but dispersive optical elements that select a single harmonic can significantly reduce the photon flux and time resolution. Here we show that 400 nm driven high harmonic extreme-ultraviolet trARPES is superior to using 800 nm laser drivers since it eliminates the need for any spectral selection, thereby increasing photon flux and energy resolution to <150 meV while preserving excellent time resolution of about 30 fs.
Ključne besede: angle-resolved photoemission spectroscopy, ARPES, high harmonic generation, ultrafast surface science
Objavljeno v RUNG: 20.04.2017; Ogledov: 5287; Prenosov: 0
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4759. Literary links between Trieste and Buenos Aires during the first half of the 20th centuryAna Toroš, 2017, objavljeni povzetek znanstvenega prispevka na konferenci Opis: The article ventures into the field of Slovene and Friulian migration to Argentina at the end of the 19th and beginning of the 20th century, speaking up on migration from the Brda region and its environs. It is a hilly and wine-growing region which was under Austro-Hungarian rule during the end of the 19th century, but after WWI the Kingdom of Italy was given the reins. Nowadays, the region is divided between Slovenia (the Goriška region) and Italy (the province of Gorizia). We will focus on two literarised chronicles, created based upon the transoceanic letter correspondence and the oral tradition. The first literary chronicle Un pò di cronaca famigliare Godeas ‒ Gradnik was written by Maria Samer, a female author of Italian and Friulian decent from Trieste. Her work is unpublished and has been found only recently. The second work Nepozabljena Brda was written by Oskar Reya ‒ Kozanski, a Slovene author from Brda. The article stems theoretically from literary imagology, using the comparative method to study the literary image of Argentina in both texts. While doing so, based on Kozanski’s chronicle, the article reveals the identity crisis specific to the migrants from Brda, who were existentially tied to their “native” land as a means of survival. This article also ventures into the field of literary reception, reconstructing the previously unknown Slovene-Argentinean literary and family connections based on Maria Samer’s chronicle. During the first half of the 20th century, Eduardo Dughera, an Argentinean author of Friulian descent, corresponded with his cousin Alojz Gradnik, one of Slovenia’s greatest poets and whose mother was Friulian, and Maria Samer, their cousin who translated both their works into Italian. In relation to this it is important to mention the interest the Slovene community in Argentina had for Gradnik in the 21st century.
Ključne besede: Friulians, migration, Brda
Objavljeno v RUNG: 20.04.2017; Ogledov: 4208; Prenosov: 0
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4760. High Photon Flux 70 eV HHG Source for Applications in Molecular and Solid State PhysicsSteffen Hädrich, Jan Rothhardt, Robert Klas, Maxim Tschernajew, Armin Hoffmann, Valentina Hajek-Tadesse, Arno Klenke, Thomas Gottschall, Tino Eidam, 2016, objavljeni znanstveni prispevek na konferenci Ključne besede: Ultrafast nonlinear optics, Multiharmonic generation, Lasers, fibe
Objavljeno v RUNG: 20.04.2017; Ogledov: 4862; Prenosov: 0
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