ECOLOGICAL STUDIES OF EPIKARST COMMUNITIES IN ALPINE AND PRE-ALPINE CAVESFederica Papi
, 2016, doktorska disertacija
Opis: The karst ecosystem shows heterogeneity and variability of geology, hydrology, morphology and ecology in space and time. Chemical composition of water in karst plays a crucial role not only in dissolution of karst rocks and deposition but also in ecological processes. The heterogeneous nature of karst aquifers leads to difficulties in predicting groundwater flow and contaminant transport direction and travel times. For its position at the top of karst, epikarst represents the interface between surface and underground. Epikarst ecology, biodiversity and fauna have rarely been systematically studied. The typical absence of enterable passages makes necessary an indirect approach.
In this research, epikarst biodiversity in relation to environmental conditions was studied in seven Alpine and Pre-Alpine caves, located at different altitudes, in Slovenia and in Italy: Snežna jama na planini Arto (1556 m a.s.l.), Jama pod Babjim zobom (860 m a.s.l.), Zadlaška jama (300 m a.s.l.) and Pološka jama (500 m a.s.l.), in Slovenian Alps and Pre-alps, and Grotta A del Ponte di Veja (600 m a.s.l.), Covolo della Croce (875 m a.s.l.) and Grotta di Roverè Mille (1005 m a.s.l.) in Lessinian Massif. In these caves, percolating water was monthly monitored for fauna in the period of one year. Temperature, discharge, conductivity and pH were measured at the same time, and water samples for the laboratory analyses of total hardness, concentrations of various ions (calcium, chlorine, nitrate, sulphate and phosphate) and dissolved organic carbon were taken.
66 aquatic and terrestrial, mostly hypogean, species were found. Aquatic fauna was dominant in all caves, with the exception of Covolo della Croce. Caves located at higher elevations harbour less diverse communities. No animals were found when discharge was very low and in correspondence of moonmilk formations. The most abundant and diverse group was Copepoda, with thirteen different species and 776 individuals at different maturity stadia, totally representing 61% of the community (between two and three copepod species per cave in Slovenia and between one and six per cave in Italy).
The 62% of copepod species were stygobionts. Some copepod species (e.g.
Speocyclops infernus) have wide ecological tolerance to environmental variables, other have more restricted tolerances (e.g. Lessinocamptus insoletus). With regard of copepods, there was a good agreement between the number of observed species and the total number of predicted species, confirming that the sampling method was appropriate and sampling complete. Correlations between the copepod community structure and measured parameters, obtained with Pearson correlation coefficient and principal component analysis (PCA) show different patterns. Presence of copepods was positively correlated with conductivity and dissolved organic carbon in Grotta di Roverè Mille, and with carbonate and nitrate in Pološka jama. In other caves not so high correlations were found between copepod species and measured parameters and between pairs of species.
Investigations of the Alpine and Pre-alpine epikarst fauna can help to understand better the ecology of the epikarst fauna and its roles within the large range of different shallow subterranean habitats.
Ključne besede: Epikarst, Alpine and Pre-Alpine caves, percolating water, speleobiology, fauna.
Objavljeno: 31.08.2016; Ogledov: 1036; Prenosov: 58
Polno besedilo (2,00 MB)
SINGLE NUCLEOTIDE POLYMORPHISMS OF THE CHROMOSOME SEGREGATION GENES INVOLVED IN THE DEVELOPMENT OF GASTRIC CANCERMarija Rogar
, 2016, doktorska disertacija
Opis: INTRODUCTION. Gastric cancer represents the fifth most frequently diagnosed cancer in the world. Despite numerous research studies, mechanisms leading to disease are poorly known and unclear. At the molecular level, many changes are involved in the development of gastric cancer, including malfunction of chromosome segregation genes. These abnormalities can lead to chromosomal instability (CIN). Segregation gene function can be affected by the low penetrance errors which include polymorphisms.
AIM. The aim of this study is to examine the effect of selected polymorphisms in specific segregation genes on gastric cancer development.
HYPOTHESIS. The study focused on exploring genotypes of selected polymorphisms in specific mitotic segregation genes. Those that differ significantly between the subjects and the healthy control population, may be associated with higher risk for developing gastric cancer or with certain clinical and histopathological characteristics, and may have effect on the survival of gastric cancer patients.
METHODS. 30 polymorphisms in genes BUB1B, CASC5, ESPL1, PTTG1, SMC1A, TPX2, TTK and ZWINT were included in the study. Subjects were compared with the control group. Polymorphisms were determined using quantitative real-time PCR (qPCR), restriction fragment length polymorphism (RFLP) and DNA sequencing.
RESULTS. The association between polymorphisms rs2277559 (BUB1B), rs2241666 (ZWINT), rs11858113 (CASC5) and rs11855334 (CASC5) and increased risk of developing gastric cancer in male population was determined. As concerning rs11855334, statistically significant difference was also observed in the genotype distribution between the whole population of subjects and controls. The association between the genotypes of polymorphisms (in gene BUB1B) rs2277559, rs2290551, rs1801376, rs1047130, rs1565866, rs2277560 and Lauren classification was recognized. Genotypes of polymorphisms rs1801376 (BUB1B), rs11855334 (CASC5), rs2241666 (ZWINT), rs2910101 (PTTG1) and rs1047130 (BUB1B) are linked to different tumour differentiation grades. Survival analysis revealed association between the lymph node involvement and perineural invasion. Statistically higher frequencies of haplotypes G-A-G-T-G-G-A, G-G-A-G-A-A-G and A-G-G-T-A-G-A in gene BUB1B and of haplotypes A-A-A-C and C-C-G-T in gene ESPL1 were observed in gastric cancer patients, whereas haplotypes A-C-A-T and C-A-G-T in gene ESPL1 were significantly more frequent in the control group. Association with gastric cancer was not noted with other polymorphisms.
CONCLUSIONS. The association between specific polymorphisms of selected chromosome segregation genes and gastric cancer was recognized. Findings could provide guidelines for further research and polymorphisms linked to gastric cancer could serve as potential diagnostic and prognostic biomarkers.
Ključne besede: gastric cancer, chromosomal instability, polymorphisms, segregation genes
Objavljeno: 27.07.2016; Ogledov: 1318; Prenosov: 70
Polno besedilo (2,87 MB)
A REVERSE GENETIC SYSTEM FOR ROTAVIRUSGiuditta De Lorenzo
, 2016, doktorska disertacija
Opis: Studies on rotavirus biology have always suffered for the lack of a reverse genetics system applicable to all genome segments and independent from the use of helper virus. One of the main reasons proposed to explain the difficulties encountered is the poor expression of the transfected cDNAs.
In this work we investigated the role of 5’UTRs in rotavirus cDNAs expression in un-infected cells. We showed that in the 5’UTR of several (but not all) genome segments of rotavirus is present a 5’-terminal inhibitory motif (IM = 5’-GGY(U/A)UY-3’) that, independently from the ORF or the 3’UTR, downregulates both transcription and translation of rotavirus cDNAs when expressed exploiting T7 polymerase-encoding recombinant vaccinia virus. We mapped two mutations (insertion of a G upstream 5’UTR and the U to A mutation of the fifth nucleotide of IM) that are capable of making the inhibitory motif non-functional restoring a satisfying yield of protein synthesis. After the analysis of all genome segment 5’UTR of SA11 strain, we created two distinct sets of mutated rotavirus genome segments containing non-functional IM. We planned to employ these sets in the development of a plasmid-based reverse genetics system that exploit vaccinia virus-encoded T7 polymerase.
In an attempt to produce in vivo biotinylated Tripled-Layered Particles (TLPs), we created a recombinant VP4 fused to the Biotin Acceptor Peptide (BAP) that, when co-expressed with the biotin ligase BirA, is efficiently in vivo biotinylated. We exploited recombinant vaccinia virus system to achieve high levels of VP4-BAP and the enzyme BirA in cells. Upon infection with rotavirus, VP4-BAP would be incorporated in the newly forming viral particles. In addition, we constructed a full-length genome segment 4 cDNA encoding the recombinant VP4-BAP to be used with a VP4 temperature sensitive rotavirus in order to generate a recombinant rotavirus encoding VP4-BAP.
Thus, the results obtained allowed us to design two distinct possible ways of generating recombinant rotavirus. The first was a genetic strategy for the incorporation of an exogenous genomic segment, with the characteristic of being helper-virus free and applicable to all genomic segments. This was the consequence of the description of an inhibitory motif present in many viral mRNAs and the identification of two mutations that abrogate its inhibitory activity allowing expression of viral proteins. The second focused on the expression of a recombinant viral structural protein expressed in virus-infected cells for the consequent incorporation into newly made virions.
Ključne besede: rotavirus, UTR, reverse genetics, T7 polymerase, in vivo biotinylation
Objavljeno: 08.06.2016; Ogledov: 716; Prenosov: 56
Polno besedilo (2,87 MB)
CHARACTERIZATION OF SLOVENIAN APPLE JUICE WITH RESPECT TO ITS GEOGRAPHICAL ORIGIN AND AGRICULTURAL PRODUCTION PRACTICEKarmen Bizjak Bat
, 2016, doktorska disertacija
Opis: Determination of food authenticity is an important issue in quality control and food safety. Recent studies predict a growing demand for natural and more authentic food and beverage products. The quality and authenticity of apple juice is also of a great economic importance since the popularity and demand for apple juice consumption has increased. The growth of the market for organically produced apples and apple juice is due to the increasing demand for healthy food requirements, protection of the environment and the promotion of biotic diversity. Organic foods have a higher nutritional and health value, but they are more expensive, because their production is more difficult and less profitable. In addition to how food is produced, consumers are increasingly placing emphasis on food products of specific region, which are known for their unique natural flavours and taste.
The presented thesis is based on four separate but closely interrelated studies, in which a combination of different isotopic ratios of bioelements (2H/1H, 13C/12C, 15N/14N, 18O/16O), multi-element analysis, and major primary and secondary metabolite profiles were exploited to differentiate the geographical origin and agricultural production practice (organic vs integrated/conventional) of Slovenian apples. These parameters were used to establish the first database of authentic Slovenian apple juice, which can be used to verify the authenticity of commercially available apple juice in Slovenia.
The first preliminary study was entitled “Organic Cultivation ~ Geographical Origin (OCGO)” and was performed using apples from the 2009 growing season. Its aim was to examine the use of stable isotope and multi-element data for determining the geographical origin and agricultural production practice of fresh apple juices. Fruits of six apple (Malus domestica Borkh) cultivars (Topaz, Idared, Golden Delicious, Goldrush, Gala, Gloster) were collected from four different geographical regions of Slovenia (Alpine, Dinaric, Pannonian and Mediterranean) grown under organic and integrated/conventional orchard management systems. The results revealed that stable isotope parameters in sugar, pulp and water were the most significant variables for differentiating between the regions. Good separation was achieved between the geographical regions in Slovenia based on the δ18O and δ2H values in water and Rb and S levels in the apple fruit juice. The most significant variables that distinguished between organically and integrated/conventionally cultivated apples were the 15N/14N ratio and antioxidant activity of the apple juice. Significant differences were also observed in the ascorbic acid content of the juice. Based on these results the number and types of apples and the minimum number of samples needed from the same region for determining geographical origin were determined.
The second study was called “Organic ~ Conventional Apple Cultivation” (OCAC) and was performed in 2010 and 2011 in a Gala apple orchard. The aim was to determine the effect of different fertilizers allowed either in organic or conventional/integrated agricultural regimes on different parameters. Quality parameters, isotopic composition of C in sugars and in pulp together with N and elemental analysis were investigated. The following five fertilizers were applied: Biosol and Plantella organic (organic) and Ca cyanamide, KAN and UREA (mineral) at a rate of 60 and 120 kg of nitrogen per hectare. From the obtained data it was possible to differentiate between organic and integrated/conventional apple production when taking into account the following parameters: mass, skin and flesh firmness (SFF), total soluble solids (TSS), and the content of Cl as well as δ15N and δ13C in the pulp.
The “Organic Cultivation ~ Geographical Origin” (OCGO) study, which took place during the 2011 and 2012 growing seasons included a greater number of samples and samples from five different geographical regions: Alpine, Dinaric,
Ključne besede: apple juice, geographical origin, agricultural production practice, biomarkers, phenol compounds, elements, stable isotopes, Slovenia
Objavljeno: 07.06.2016; Ogledov: 1368; Prenosov: 187
Polno besedilo (3,97 MB)
Poezija Franceta Prešerna v srednješolskih učbenikih in njena recepcijaZoran Božič
, 2010, doktorska disertacija
Opis: Disertacija obravnava dva problemska sklopa: poezijo Franceta Prešerna v srednješolskih učbenikih za pouk književnosti od leta 1850 do leta 2010 ter recepcijo Prešernovih pesmi skozi čas in v sodobnosti. V prvem problemskem sklopu razkriva dejavnike in postopke šolske kanonizacije Prešerna ter razvoj didaktizacije beril v smeri učne knjige za samostojno delo z besedilom. V drugem problemskem sklopu raziskuje posebnosti pri razumevanju, doživljanju in vrednotenju Prešernovih pesmi ter načine za njihovo čimbolj učinkovito šolsko predstavitev.
Glede na to, da se je kanonizacija Prešerna začela že za časa njegovega življenja, je bil seveda vseh 160 let kanonski avtor, vendar pa kljub nenehnemu označevanju Prešerna za "največjega slovenskega pesnika" po številu besedilnih enot v berilih ni bil vedno prvi avtor, pač pa so ga večkrat prehitevali mlajši književniki, ki so bolj ustrezali trenutnim afinitetam sestavljavcev srednješolskih beril. Predvsem v drugi polovici 19. stoletja in tudi kasneje je bil zaradi negativnega odnosa šolskih oblasti do ljubezenske poezije izbor Prešernovih pesmi praviloma manj primeren (na nižji stopnji je bil največkrat objavljen sonet Memento mori, na višji stopnji pa cikel Soneti nesreče), nekatere ljubezenske pesmi pa so celo cenzurirali.
Od druge polovice 19. stoletja do danes se je razvijal didaktični instrumentarij srednješolskih beril (najprej so začeli dodajati stvarne opombe, kasneje pa še vprašanja in naloge za dejavno razčlembo leposlovnega besedila), vendar so še zlasti v obdobju po drugi svetovni vojni nekateri sestavi beril didaktično pomenili izrazito nazadovanje. Od sedemdesetih let dalje berila vključujejo tako literarnovedne vsebine kot vprašanja in naloge, sodobna berila pa se praviloma (izjema je npr. antološki sestav Krakar 2003) razvijajo v smeri vezanega berila z relativno majhno ponudbo leposlovnih besedil.
Zgodovinski pregled je pokazal, da so imeli težave z razumevanjem Prešernovih pesmi že njegovi sodobniki, kasneje pa tudi izobraženci, celo poznavalci Prešerna. Empirični preizkusi med slovenskimi srednješolci potrjujejo visoko recepcijsko zahtevnost Prešernove poezije. Nekatere osrednje Prešernove pesmi (npr. Krst pri Savici) zahtevajo izjemno gostoto dodanih opomb, zato je najustreznejše didaktično sredstvo za boljše razumevanje in doživljanje oblikovno in slogovno zahtevne klasične poezije dodana prozifikacija, kar so v celoti potrdili tudi rezultati pedagoškega eksperimenta.
Ključne besede: poezija, Prešeren, didaktika, recepcija, kanonizacija, srednja šola, berilo, kanonizacija, prozifikacija, pedagoški eksperiment
Objavljeno: 12.05.2016; Ogledov: 668; Prenosov: 17
Polno besedilo (10,63 MB)
ROLE OF TDP-43 AGGREGATION IN NEURODEGENERATION: A DROSOPHILA MELANOGASTER DISEASE MODEL AND INNOVATIVE THERAPEUTIC APPROACHESLucía Cragnaz
, 2016, doktorska disertacija
Opis: TDP-43 inclusions are important histopathological features of various neurodegenerative disorders, including Amyotrophic Lateral Sclerosis. However, the relation of these inclusions with the pathogenesis of the disease is still unclear. Various hypotheses have been proposed. For instance, it was suggested that the inclusions are (1) primary toxic species, (2) part of the normal cellular protective response to toxic intermediates and (3) responsible for the nuclear depletion of TDP-43. Understanding the relationship between TDP-43 aggregation and neurodegeneration is crucial for the eventual management of the disease.
TDP-43 is a protein that has a marked tendency to unfold and become insoluble. In particular, its C-terminal end has a so-called “prion-like domain”, a sequence rich in Glutamine (Q) and Asparagine (N) that is involved in both the interactions with other proteins and the self-aggregation process. A cellular model of aggregation has been previously developed by our group, using the TDP-43 Q/N rich amino acid sequence repeated 12 times (12xQ/N) fused to EGFP reporter. The EGFP-12xQ/N cellular inclusions are capable of sequestering wild type TDP-43 both in non-neuronal and neuronal cells.
In this study we went further with ALS modeling, creating a Drosophila model with EGFP-12xQ/N-induced aggregates. We show here that Drosophila melanogaster TDP-43 ortholog (TBPH) overexpression in Drosophila eye using GMR-Gal4 driver, is neurotoxic and causes necrosis and loss of function of the eye. More important, the neurotoxicity of TBPH can be abolished by its incorporation to the insoluble aggregates induced by EGFP-12xQ/N. This data indicates that aggregation is not toxic per se and instead has a protective role, modulating the functional TBPH available in the tissue.
Notwithstanding the fact that aggregation is protective in presence of an excess of TBPH, we wanted to further understand the role of the aggregates in an environment where just the endogenous TBPH is present. For this purpose, we induced EGFP-12xQ/N transgene constitutively in CNS using ELAV-Gal4 driver. The flies were born and went through the larval stage without differing from control flies in any significant feature of their
phenotype. However, during aging the locomotion ability and survival rate of EGFP-12xQ/N flies were impaired. Interestingly, the climbing deficit was correlated with a physiological reduction in the endogenous TBPH levels. Thus, the aggregation, when coupled with low TDP-43 levels generates phenotypic consequences in our Drosophila model, probably due to a TDP- 43 loss of function.
In sum, these data suggest that although the aggregates may be a result of neuroprotection in a context where TBPH is in excess, at a certain stage they become responsible for the pathology, likely due to the TBPH loss of function. If we consider that TDP-43/TBPH inclusions act as a sink for the newly formed soluble TDP-43/TBPH, the modulation of these inclusions could be used as a potential therapeutic approach, as this would restore the normal levels of TDP-43/TBPH and its function. Consequently, in the last part of the study we were interested to understand if the clearance of TDP- 43/TBPH aggregates could be an effective strategy to treat ALS, by recovering TDP-43/TBPH function. For this purpose, using the previously established cell-based TDP-43 aggregation models we analyzed aggregate clearance after treatment with several FDA approved drugs. Three of these drugs were found to significantly reduce aggregation through the proteasome pathway. Furthermore, one of the drugs (nortriptyline) was shown to rescue EGFP-12xQ/N dependent locomotor dysfunction in the Drosophila model. Altogether these data indicate that the clearance of TDP-43 aggregates may be a novel therapeutic strategy for ALS treatment.
Ključne besede: TDP-43/TBPH, aggregation, Drosophila melanogaster, ALS
Objavljeno: 03.03.2016; Ogledov: 1265; Prenosov: 8
Polno besedilo (53,65 MB)
CAPOEIRA: CONCEPTUALIZATION AND PRESENTATION OF THE BODYUrška Stolnik
, 2015, doktorska disertacija
Opis: This thesis is the result of twenty-one months of fieldwork among the capoeiristas in Brazil, primarily in the city of Salvador, between 2003 and 2013. Combining historical accounts with the experiences of the present, the work focuses on changes in the social perception of body and mind in both capoeira and wider society. The intricacies of society and the politics of a particular epoch are clearly reflected in this Brazilian national game: sometimes in terms of incorporation or acceptance of specific social hierarchies, and other times of exclusion or rejection of them. A tension between the black and white worlds is constantly present, as it is between the male and female ones. Even when these separate realms in different historical eras of capoeira seem to come closer, they become separated again through new forms of institutionalization. Perceived sometimes as a martial art or dance, sometimes as a game, and yet other times as a sport through which one can gain health and beauty, capoeira evades a firm and permanent definition. Such evasion resembles capoeira’s three main, cunning characteristics: malandragem (trickery), malícia (deception) and mandinga (seduction).
Cunningness, as a tactic to overplay the opponent, is a means of intentional opening and closing of the body in the game. Although opening and closing might seem exclusive, they are, on the contrary, very much inseparable. They coexist in symbiosis not just at the level of a capoeirista playing the game, but also at the level of roda, academia, a capoeira group or school, at the level of capoeira as a practice, and finally, at the level of wider Brazilian society. All of these bodies are striving to be fechado (closed) and secure, but cannot accomplish that before opening themselves first.
Being dexterous in the simultaneous alternation of opening and closing one’s own body is a skill that can be learnt through a long-term and continually engendered process of practice, experiences and interaction with others and the environment at large. That skill, associated with the capoeirista’s awareness of
constant vulnerability, is closely related to learning the specific way of perceiving and responding. The capoeirista cultivates and embodies capoeiristic habitus, which emphasizes the necessity of being permanently attentive to several things at once. This is possible when all senses work together in synthesis. At that time, the capoeirista uses peripheral sight and is aware of peripheral sounds. The skill of heightened sensitivity and keen perception enables the capoeirista to negotiate between the opening and closing of his body. Opening and closing the body at the right time and taking the advantages of an unstable situation in roda can be accomplished only by simultaneous thinking and performing, which is the outcome of the incorporation of capoeira movements.
The socio-economic situation of Brazilian society based on racial and gender discrimination enhances the cultivation of capoeiristic habitus and dictates the need for the acquisition of capoeiristic skills and attitudes in order to cope with, understand, and live within an unequal world. Capoeiristic habitus can also be learnt and acquired as a by-product of capoeira training. But it remains important whether the environment in which a capoeirista practises tolerates or inhibits the cultivation of capoeiristic skills.
Ključne besede: capoeira, body and mind, game, sport, movement, opened and closed society, perception, historical changes, Salvador, Brazil
Objavljeno: 22.01.2016; Ogledov: 1316; Prenosov: 72
Polno besedilo (4,71 MB)
V očeh razpadajočega imperija: Podoba Slovenije v Sovjetski zvezi in Rusiji v času slovenskega osamosvajanja (1990 – 1992)Andrej Stopar
, 2015, doktorska disertacija
Opis: Za mlado državo je priznanje mednarodne skupnosti ključnega pomena. Tudi za Republiko Slovenijo je bilo. Sovjetska zveza je odločno podpirala enotnost in ozemeljsko celovitost SFR Jugoslavije, katere razpad je obravnavala v kontekstu lastne dezintegracije. Disertacija V očeh razpadajočega imperija: Podoba Slovenije v Sovjetski zvezi in Rusiji v času slovenskega osamosvajanja (1990 – 1992) tematizira sovjetske in ruske poglede na razpad SFR Jugoslavije in jugoslovanskega sistema ter na slovenska prizadevanja za rusko priznanje neodvisnosti in suverenosti republike. Pri tem se opira na gradivo najpomembnejših sovjetskih in ruskih tiskanih medijev v obdobju 1990 – 1992, na sovjetske in ruske diplomatske vire, uradne izjave, komentarje akademskih krogov ter spominske in dnevniške zapise. Disertacija skuša pokazati, da je bila odločitev Moskve posledica kratkotrajnega sovpadanja določenih mednarodnopolitičnih razmer, takrat nove ruske zunanjepolitične strategije, ki se je hitro spremenila in je kot takšna pomenila izjemo v ruski tradiciji zunanjepolitičnih konceptov, ter predvsem notranjepolitičnih napetosti v Ruski federaciji po razpadu Sovjetske zveze.
Ključne besede: Sovjetska zveza, Rusija, Jugoslavija, Slovenija, diplomatski odnosi, mediji, razpad socialističnih federacij
Objavljeno: 22.01.2016; Ogledov: 1432; Prenosov: 86
Polno besedilo (2,02 MB)
Water salinity and the efficiency of constructed wetlandsMilana Karajić
, 2015, doktorska disertacija
Opis: Raziskava teze je bila osredotočena na vpliv slanosti na učinkovitost rastlinske čistilne naprave. Raziskava je bila izvedena na rastlinski čistilni napravi Dragonja ki se nahaja na Jadranski obali, na jugo-zahodu Slovenije in na pilotskem modelu rastlinske čistilne naprave. Rezultati raziskave sugerirajo, da povečanje slanosti negativno vpliva na učinkovitost skoraj vseh spremljanih parametrov (amonijaka, fosfata, nitrata, nitrita, COD in BOD5), ampak korelacija ni bila signifikantna. Majhni koeficienti korelacije pomenijo, da koncentracije kloridov in prevodnost lahko razložijo majhen delež variabilnosti učinkovitosti rastlinske čistilne naprave Dragonja. Mogoče ima prisotnost različnih koncentracij drugih polutantov v izcedni vodi, ki jih nismo spremljali v nalogi, inhibitorni vpliv na mikroorganizme v rastlinski čistilni napravi in tako negativno vpliva na učinkovitost rastlinske čistilne naprave Dragonja.
Za raziskavo procesa čiščenja slane odpadne vode so bili v substratu pilotskega modela rastlinske čistilne naprave inokulirani halotolerantni mikroorganizmi in spremljana učinkovitost čiščenja odpadne vode. Vpliv slanosti na učinkovitost, preživetje in uporabo halotolerantnih mikroorganizmov je bil preverjen pri 0%, 1,5% in 3,0% NaCl v odpadni vodi. Prav tako smo raziskali vpliv aeracije ter vpliv aeracije z dodano saharozo (2g/l) v sintetični odpadni vodi. V substratu pilotskega modela peščenega filtra rastlinske čistilne naprave smo spremljali spremembe v pH, prevodnosti, redoks potencialu, koncentraciji kisika, amonijevih ionov, kloridnih ionov, fosfatnih ionov, KPK, BPK5 in ETS aktivnosti, koncentraciji CO2 v vodi in koncentraciji CO2. Ugotovili smo, da je na učinkovitost pilotskega modela, inokuliranega s halotolerantnimi mikroorganizmi, večji vpliv imela aeracija in prisotnost saharoze (kot organskega izvira ogljika), kot spremembe v slanosti vode.
Ključne besede: rastlinske čistilne naprave, slana odpadna voda, halotolerantni mikroorganizmi
Objavljeno: 22.12.2015; Ogledov: 1073; Prenosov: 21
Polno besedilo (6,15 MB)