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91.
Nanobodies: towards rational design of immune-reagents
Ario De Marco, 2017, published scientific conference contribution abstract (invited lecture)

Abstract: Antibodies are irreplaceable reagents in both research and clinical practice. Despite their relevance, the structural complexity of conventional mono- and polyclonal antibodies (IgG) has always been a limit for their engineering towards reagents optimized for specific applications, such as in vivo diagnostics and therapy. Furthermore, their isolation is time consuming, their production expensive, and their functionalization results often in heterogeneous macromolecule populations. These drawbacks promoted the search for both innovative antibody isolation strategies and alternative scaffolds. In vitro panning of pre-immune collections of recombinant antibody fragments allows for the simple and fast recovery of binders. Since they did not undergo somatic maturation, their affinity for targets can be insufficient but on the other hand they can be rapidly mutated by standard molecular biology techniques to generate second-generation antibodies among which to identify clones with improved characteristics. Both stochastic and rational methods have been proposed for the optimization process. Random mutagenesis followed by panning at stringent conditions has been successful used to select binders with improved physical characteristics. Rational methods try to identify in silico key residues involved in the regulation of specific antibody features, such as stability or binding affinity. The accuracy of these methods usually depends on the calculation resources. In this perspective, smaller molecules can be analyzed “better” than larger because of their restricted number of residues. Nanobodies small dimensions have been long appreciated since enable better tissue penetration, shorter clearance time, higher yields. Now it becomes evident that this characteristic makes them also optimal objects for modeling.
Keywords: recombinant antibody modeling, nanobody engineering, molecular dynamics and docking
Published in RUNG: 21.03.2018; Views: 4376; Downloads: 0
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92.
Nanobodies: flexible structures for biotechnological applications
Ario De Marco, unpublished invited conference lecture

Keywords: nanobodies, in vitro panning, extracellular vesicles, oncology, toxic algae
Published in RUNG: 16.03.2018; Views: 3620; Downloads: 0
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93.
94.
A caccia di proteine usando gli anticorpi come esca
Ario De Marco, unpublished invited conference lecture

Keywords: Fluorescent proteins, antibodies, microscopy, diagnostics
Published in RUNG: 13.02.2018; Views: 3608; Downloads: 0
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95.
96.
97.
Naked protein nanoparticles
Ario De Marco, 2017, published scientific conference contribution abstract (invited lecture)

Keywords: Protein nanoparticles, targeted delivery, cell uptake
Published in RUNG: 15.12.2017; Views: 3354; Downloads: 415
.pdf Full text (2,09 MB)

98.
In vitro isolation of anti Alexandrium minutum nanobodies from a pre-immune library
Elisa Mazzega, Alfred Beran, Marina Cabrini, Ario De Marco, 2017, published scientific conference contribution abstract

Keywords: nanobodies, toxic microalgae, biopanning
Published in RUNG: 04.12.2017; Views: 4106; Downloads: 33
URL Link to full text
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99.
100.
Engineered cross-reacting nanobodies simplify comparative oncology between humans and dogs
Elisa Mazzega, Ario De Marco, 2017, original scientific article

Keywords: HER2, nanobodies, inter-species cross-reactivity, thermophoresis
Published in RUNG: 19.10.2017; Views: 4508; Downloads: 0
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