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Toxins in microalgae
Tanja Batkovič, Mojca Zotler, Meta Križaj, Jan David, Dani Bratuž, Anže Kuraj, 2017, final research report

Abstract: The aim of the project is to develop a detection system for the toxic algae Alexandrium minutum which can be than used as part of biosensor. First, we will isolate a single-domain antibody from a pre-immune library, then subclone its sequence in different vectors and produce it. Finally, we will design alternative ELISA methods and choose the most suitable to quantify the microalgae in water samples.
Found in: osebi
Keywords: Alexandrium minutum, mikroalge, biosenzorji ELISA
Published: 02.11.2018; Views: 2292; Downloads: 0
.pdf Fulltext (5,00 MB)

Recombinant nanobodies as cheap and customizable reagents for unicellular algae detection
Elisa Mazzega, Ario De Marco, Marina Cabrini, Alfred Beran, published scientific conference contribution abstract

Abstract: At the present, the identification of planktonic species in coastal water mainly relies on light microscopy observations. This kind of analyses is performed by highly trained personnel, requires lab equipment and long processing time. High-throughput and easy-to-perform methods are instead highly needed for routine costal and ballast water monitoring. Immuno-reagents are widely employed in the medical field for routine diagnostics, where they provide the necessary sensitivity and specificity, as for example for cancer subtype characterization. Reagents of similar grade are so far not widely available for both diagnostics and basic research of microalgae. We describe the first successful isolation of a single-domain antibody (nanobody or VHH) from a pre-immune library, its engineering into application-ready reagents, and its inexpensive production as recombinant fusion protein. Alexandrium minutum was chosen as a model organism to test the feasibility of the procedure. The procedure foresees the panning of a pre-immune phage library of VHHs that was used for in vitro selection against directly the target cells. Monoclonal nanobodies specific for A. minutum cells were identified and optimized for recombinant production as fusion with fluorescent proteins in bacterial hosts. Such fluorescently-tagged VHHs were validated by immunofluorescence and cytofluorimetry for their selectivity by testing unicellular algal species that can be found in the same environment of A. minutum. Two nanobodies were found to be highly specific for the target cells, were able to bind also cysts of A. minutum and they gave no cross-reaction, even for a not-toxic strain of the closely related A. tamutum. Different tags can be then fused to the selected nanobodies and used instead of the fluorescent proteins to obtain a reagent immediately applicable to further techniques, such as cell Enzyme Linked Immuno Sorbent Assay (ELISA) or biosensor surface functionalization. The newly produced reagents can be applied for direct whole-cell detection in seawater, bypassing the need of cell processing required for DNA or RNA diagnostics, and can be used for both alive and fixed cells, guaranteeing the possibility to check old samples and to perform confirmatory morphological studies.
Found in: osebi
Keywords: Nanobodies, toxic algae, detection, recombinant reagent, naive library, phage display
Published: 21.12.2018; Views: 2427; Downloads: 0
.pdf Fulltext (4,97 MB)

Multi-disciplinary approaches for designing new generation antibodies
Ario De Marco, unpublished conference contribution

Found in: osebi
Keywords: NMR, Cross-linking, Nanobodies
Published: 17.04.2019; Views: 1786; Downloads: 0
.pdf Fulltext (587,23 KB)

Nanobodies: How to Exploit Pre-Immune Libraries and Recombinant Antibody Fragments
Ario De Marco, unpublished invited conference lecture

Found in: osebi
Keywords: nanobodies, in vitro panning, phage display
Published: 23.08.2019; Views: 1889; Downloads: 0
.pdf Fulltext (188,41 KB)

Nanobodies as a flexible biotechnological platform
Ario De Marco, invited lecture at foreign university

Found in: osebi
Keywords: nanobodies, phage display, crystallography chaperones, functionalization strategies
Published: 10.11.2019; Views: 1681; Downloads: 0
.pdf Fulltext (933,86 KB)

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