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11.
Mediterranean Coastal Lagoons: The Importance of Monitoring in Sediments the Biochemical Composition of Organic Matter
Monia Renzi, Francesca Provenza, Sara Pignattelli, Lucrezia Cilenti, Antonietta Specchiulli, Milva Pepi, 2019, izvirni znanstveni članek

Opis: Transitional water ecosystems are targeted by the European Union (EU) Water Framework Directive (WFD, CE 2000/60) monitoring programs in coastal zones. Concerning sediments, activities performed for the WFD focus on a few variables concerning the biochemical composition of organic matter. Our research reports the effects of oxygen availability on the biochemical composition of organic matter in sediments to highlight levels of targeted variables in time and, according to the depth of sediment layer, both under oxygenated and anoxic conditions in a mesocosm study on sediment cores. Results provide evidence that tested factors of interest (i.e., disturbance type, oxygenic versus anoxic conditions; persistence time of disturbance, 0–14 days; penetration through sedimentary layers, 0–10 cm depth) are able to significantly affect the biochemical composition of organic matter in sediments. Large part of the variables considered in this study (total organic carbon (TOC), total phosphorous (TP), total sulphur (TS), Fe, carbohydrates (CHO), total proteins (PRT), biopolymeric carbon (BPC), chlorophyll-a (Chl-a) are significantly affected and correlated to the oxygenation levels and could be good early indicators of important changes of environmental conditions. Monitoring activities performed under WFD guidelines and management strategies of Mediterranean coastal lagoon ecosystems shall include the biochemical composition of organic matter in sediment to provide an exhaustive picture of such dynamic ecosystems.
Najdeno v: osebi
Ključne besede: decomposition, transitional water ecosystems, organic loads, mesocosm, monitoring programs
Objavljeno: 20.04.2020; Ogledov: 1648; Prenosov: 0
.pdf Polno besedilo (2,22 MB)

12.
A multielement analysis of Cu induced changes in the mineral profilesof Cu sensitive and tolerant populations of Silene paradoxa L.
Sara Pignattelli, Ilaria Colzi, Antonella Buccianti, Ilenia Cattani, Gian Maria Beone, Henk Schat, Cristina Gonnelli, 2013, izvirni znanstveni članek

Opis: tThis work investigates the Cu induced changes in element profiles in contrasting ecotypes of Silene para-doxa L. A metallicolous copper tolerant population and a non-metallicolous sensitive population weregrown in hydroponics and exposed to different CuSO4treatments. Shoot and root concentrations of Ca,Cu, Fe, K, Mg, Mn, Mo, Na, P, S and Zn were evaluated through ICP-OES.Results indicated that increasing the environmental Cu concentration had a population dependenteffect on element profiles, shoot-to-root ratios and correlations among the elements. Generally, in thetolerant population Cu treatment induced a higher element accumulation in roots and had minimaleffects on the shoot element profile, thus resulting in a progressively decreasing shoot-to-root ratio foreach element. In the sensitive population element concentrations in root and shoot were much moreaffected and without a consistent trend. Copper treatment also affected the correlations between theelements, both in roots and shoots of the two populations, but more so in the sensitive population thanin the tolerant one. Thus, Cu exposure strongly disturbed element homeostasis in the sensitive population,but barely or not in the tolerant one, probably mainly due to a higher capacity to maintain proper rootfunctioning under Cu exposure in the latter. Differences in element profiles were also observed in theabsence of toxic Cu exposure. These differences may reflect divergent population-specific adaptations todifferential nutrient availability levels prevailing in the populations’ natural environments. There is noevidence of inherent side-effects of the Cu tolerance mechanism operating in the tolerant population.
Najdeno v: osebi
Ključne besede: Mineral profile, Copper tolerance, Silene paradoxa, Compositional data analysis
Objavljeno: 20.04.2020; Ogledov: 1739; Prenosov: 0
.pdf Polno besedilo (5,47 MB)

13.
Exploring element accumulation patterns of a metal excluder plant naturally colonizing a highly contaminated soil
Sara Pignattelli, Ilaria Colzi, Lorenzo Cecchi, Miluscia Arnetoli, Roberto Monnanni, Roberto Gabbrielli, Cristina Gonnelli, 2012, izvirni znanstveni članek

Opis: This work investigates the element distribution in Silene paradoxa growing on the mine dump of Fenice Capanne (Tuscany, Italy). The accumulation of As, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb and Zn in root apoplast and symplast and in shoot was assessed and compared to the levels of the same metals in the respective rizosphere soils, analyzing both the total and the phytoavailable fractions. Levels of As, Cu, Fe, Pb and Zn, were above toxicity thresholds in both soil and shoot samples. Inter- and intra-element correlations were analyzed in plant and soil using different statistical methods. Soil total and phytoavailable metal concentration were shown not to be dominant in determining metal accumulation by the plant, since no significant positive correlation was found between metal concentration in soils and plants. Moreover, results indicated that S. paradoxa was able to cope with the studied multi-metal contaminated soil excluding the elements from its tissues and preferentially accumulating them into the root compartment, thus suggesting this species as possible good candidate for phytostabilization purposes.
Najdeno v: osebi
Ključne besede: Metals, Mine dump, Phytostabilization, Silene paradoxa, Compositional data analysis
Objavljeno: 20.04.2020; Ogledov: 1704; Prenosov: 0
.pdf Polno besedilo (4,42 MB)

14.
Short-term physiological and biometrical responses of Lepidium sativum seedlings exposed to PET-made microplastics and acid rain
Serena Felline, Antonio Terlizzi, Manuela Piccardo, Andrea Broccoli, Sara Pignattelli, Monia Renzi, 2021, izvirni znanstveni članek

Opis: Plastics enter in terrestrial natural system primarily by agricultural purposes, while acid rain is the result of anthropogenic activities. The synergistic effects of microplastics and acid rain on plant growth are not known. In this study, different sizes of polyethylene terephthalate (PET) and acid rain are tested on Lepidium sativum, in two separate experimental sets. In the first one we treated plants only with PET, in the second one we used PET and acid rain together. In both experimentations we analyzed: i) plant biometrical parameters (shoot height, leaf number, percentage inhibition of seed germination, fresh biomass), and ii) oxidative stress responses (hydrogen peroxide; ascorbic acid and glutathione). Results carried out from our experiments highlighted that different sizes of polyethylene terephthalate are able to affect plant growth and physiological responses, with or without acid rain supplied during acute toxicity (6 days). Short description: This study showed that different sizes of PET microplastics affect physiological and biometrical responses of Lepidum sativum seedlings, with or without acid rain; roots and leaves responded differently
Najdeno v: osebi
Ključne besede: Polyethylene terephthalate Acid rain Oxidative stress Biometrical response Lepidium sativum
Objavljeno: 16.12.2020; Ogledov: 1238; Prenosov: 0
.pdf Polno besedilo (1,14 MB)

15.
Effects of polyethylene terephthalate (PET) microplastics and acid rain on physiology and growth of Lepidium sativum
Monia Renzi, Sara Pignattelli, Andrea Broccoli, Manuela Piccardo, Antonio Terlizzi, 2021, izvirni znanstveni članek

Opis: This study evaluated the chronic toxicity (30 days) of different sizes of polyethylene terephthalate (PET) microplastics (60e3000 mm) provided alone or in combination with acid rain, on garden cress (Lepidium sativum). Both biometrical and physiological traits have been evaluated: i) percentage inhibition of seed germination, plant height, leaf number and fresh biomass production; ii) oxidative stress responses (hydrogen peroxide; ascorbic acid and glutathione production); iii) impairment in photosynthetic machinery in term of pigments production; iv) aminolevulinic acid and proline production. Results highlighted that different sizes of PET, alone or in combination with acid rain, are able to negatively affect both biometrical and physiological plant traits. In particular, the lower size of microplastics is able to negatively affect growth and development, as well as to trigger the oxidative burst. Regarding the pigments production, PET coupled with acid rain, induced a higher production of Chl-b, and an inhibition of aminolevulinic acid.
Najdeno v: osebi
Ključne besede: polyethylene terephthalate, acid rain, lepidium sativum, oxidative burst, germinability, chlorophylls
Objavljeno: 02.04.2021; Ogledov: 896; Prenosov: 0
.pdf Polno besedilo (688,72 KB)

16.
Identification of environmental stress conditions by comparative analysis of peroxidase activity in betulla pendula and pinus sp.
Kenan Kapetanović, Hena Zukić, Miroslav Štrbac, Darian Rampih, Katarina Erker, Nina Žvab-Pernat, Patrik Cingerli, 2021, elaborat, predštudija, študija

Opis: The study investigated the possibility of developing a biomarker to evaluate the stress level in trees. The analyses have been performed on tree samples appertaining to two different tree species and collected at two locations in Slovenia. The presence and the activity of the proteins – peroxidases was measured by the qualitative method of the agarose gel electrophoresis and the quantitative method of spectrometry. It was found out that by measuring the presence of the biomarkers and pigments in the tree samples we can determine the stress levels and thus the health status of trees.
Najdeno v: osebi
Ključne besede: biomarkers, environmental stress, pigments, peroxidase
Objavljeno: 29.09.2021; Ogledov: 629; Prenosov: 0
.pdf Polno besedilo (467,93 KB)

17.
Real-time monitoring of Arundo donax response to saline stress through the application of in vivo sensing technology
Andrea Zappettini, Janni Michela, Claudia Cocozza, Federico Brilli, Sara Pignattelli, Filippo Vurro, Nicola Coppede, Manuele Bettelli, Davide Calestani, Francesco Loreto, 2021, izvirni znanstveni članek

Opis: One of the main impacts of climate change on agriculture production is the dramatic increase of saline (Na+) content in substrate, that will impair crop performance and productivity. Here we demonstrate how the application of smart technologies such as an in vivo sensor, termed bioristor, allows to continuously monitor in real-time the dynamic changes of ion concentration in the sap of Arundo donax L. (common name giant reed or giant cane), when exposed to a progressive salinity stress. Data collected in vivo by bioristor sensors inserted at two different heights into A. donax stems enabled us to detect the early phases of stress response upon increasing salinity. Indeed, the continuous time-series of data recorded by the bioristor returned a specific signal which correlated with Na+ content in leaves of Na-stressed plants, opening a new perspective for its application as a tool for in vivo plant phenotyping and selection of genotypes more suitable for the exploitation of saline soils.
Najdeno v: osebi
Ključne besede: Arundo donax, saline stress, vivo sensing technology
Objavljeno: 17.12.2021; Ogledov: 584; Prenosov: 1
URL Polno besedilo (0,00 KB)
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18.
Impact of microplastics on growth, photosynthesis and essential elements in Cucurbita pepo L.
Stefano Loppi, Cristina Gonnelli, Andrea Coppi, Sara Pignattelli, Elisabetta Bianchi, Maria Beatrice Castellani, Ilaria Colzi, Luciana Renna, 2022, izvirni znanstveni članek

Opis: In this study, Cucurbita pepo L., one of the most cultivated, consumed and economically important crop worldwide, was used as model plant to test the toxic effects of the four most abundant microplastics identified in contaminated soils, i.e. polypropylene (PP), polyethylene (PE), polyvinylchloride (PVC), and polyethyleneterephthalate (PET). Cucurbita plants were grown in pots with increasing concentrations of the microplastics, then plant biometry, photosynthetic parameters and ionome of treated vs. untreated samples were compared to evaluate the toxicity of each plastic. All the pollutants impaired root and, especially, shoot growth. Specific and concentration-dependant effects of the different microplastics were found, including reduction in leaf size, chlorophyll content and photosynthetic efficiency, as well as changes in the micro- and macro-elemental profile. Among all the microplastics, PVC was identified as the most toxic and PE as the less toxic material. PVC decreased the dimensions of the leaf lamina, the values of the photosynthetic performance index and the plant iron concentration to a higher extent in respect to the other treatments. Microplastic toxicity exerted on the growth of C. pepo raises concerns about possible yield and economic loss, as well as for risks of a possible transfer into the food chain.
Najdeno v: osebi
Ključne besede: microplastic toxicity, leaf traits, chlorophyll fluorescence, ionome, zucchini
Objavljeno: 17.12.2021; Ogledov: 554; Prenosov: 1
URL Polno besedilo (0,00 KB)
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