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Structural basis of human PCNA sliding on DNA
Matteo De March, Nekane Merino, Susana Barrera-Vilarmau, Ramon Crehuet, Silvia Onesti, Francisco J. Blanco-Silva, Alfredo De Biasio, 2017, izvirni znanstveni članek

Ključne besede: structure, sliding, PCNA
Objavljeno v RUNG: 03.03.2021; Ogledov: 1894; Prenosov: 15
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Simultaneous ternary extension of DNA catalyzed by a trimeric replicase assembled in vivo
Alejandro Montón Silva, Fabio Lapenta, Alessandra Stefan, Fabrizio Dal Piaz, Alessandro Ceccarelli, Alessandro Perrone, Alejandro Hochkoeppler, 2015, izvirni znanstveni članek

Opis: According to current models, dimeric DNA Polymerases coordinate the replication of DNA leading and lagging strands. However, it was recently shown that trimeric DNA Polymerases, assembled in vitro, replicate the lagging strand more efficiently than dimeric replicases. Here we show that the τ, α, ε, and θ subunits of Escherichia coli DNA Polymerase III can be assembled in vivo, yielding the trimeric τ3α3ε3θ3 complex. Further, we propose a molecular model of this complex, whose catalytic action was investigated using model DNA substrates. Our observations indicate that trimeric DNA replicases reduce the gap between leading and lagging strand synthesis.
Ključne besede: Trimeric DNA Polymerase, E. coli, DNA Polymerase III, τ3α3ε3θ3 complex, Triple DNA extension
Objavljeno v RUNG: 27.01.2021; Ogledov: 1906; Prenosov: 0
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