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51.
Nanobodies: towards rational design of immune-reagents
Ario De Marco, 2017, objavljeni povzetek znanstvenega prispevka na konferenci (vabljeno predavanje)

Opis: Antibodies are irreplaceable reagents in both research and clinical practice. Despite their relevance, the structural complexity of conventional mono- and polyclonal antibodies (IgG) has always been a limit for their engineering towards reagents optimized for specific applications, such as in vivo diagnostics and therapy. Furthermore, their isolation is time consuming, their production expensive, and their functionalization results often in heterogeneous macromolecule populations. These drawbacks promoted the search for both innovative antibody isolation strategies and alternative scaffolds. In vitro panning of pre-immune collections of recombinant antibody fragments allows for the simple and fast recovery of binders. Since they did not undergo somatic maturation, their affinity for targets can be insufficient but on the other hand they can be rapidly mutated by standard molecular biology techniques to generate second-generation antibodies among which to identify clones with improved characteristics. Both stochastic and rational methods have been proposed for the optimization process. Random mutagenesis followed by panning at stringent conditions has been successful used to select binders with improved physical characteristics. Rational methods try to identify in silico key residues involved in the regulation of specific antibody features, such as stability or binding affinity. The accuracy of these methods usually depends on the calculation resources. In this perspective, smaller molecules can be analyzed “better” than larger because of their restricted number of residues. Nanobodies small dimensions have been long appreciated since enable better tissue penetration, shorter clearance time, higher yields. Now it becomes evident that this characteristic makes them also optimal objects for modeling.
Najdeno v: osebi
Ključne besede: recombinant antibody modeling, nanobody engineering, molecular dynamics and docking
Objavljeno: 21.03.2018; Ogledov: 1648; Prenosov: 0
.pdf Polno besedilo (638,20 KB)

52.
In vitro isolation of anti Alexandrium minutum nanobodies from a pre-immune library
Ario De Marco, Marina Cabrini, Alfred Beran, Elisa Mazzega, 2017, objavljeni povzetek znanstvenega prispevka na konferenci

Najdeno v: osebi
Ključne besede: nanobodies, toxic microalgae, biopanning
Objavljeno: 04.12.2017; Ogledov: 1466; Prenosov: 10
.pdf Polno besedilo (61,76 KB)
Gradivo ima več datotek! Več...

53.
Naked protein nanoparticles
Ario De Marco, 2017, objavljeni povzetek znanstvenega prispevka na konferenci (vabljeno predavanje)

Najdeno v: osebi
Ključne besede: Protein nanoparticles, targeted delivery, cell uptake
Objavljeno: 15.12.2017; Ogledov: 1170; Prenosov: 323
.pdf Polno besedilo (2,09 MB)

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55.
Nanomaterial bio-activation and macromolecules functionalization: the search for reliable protocols
Ario De Marco, 2018, pregledni znanstveni članek

Najdeno v: osebi
Ključne besede: protein functionalization, tags, site-directed protein labeling, sample homogeneity
Objavljeno: 28.02.2018; Ogledov: 1273; Prenosov: 0
.pdf Polno besedilo (419,88 KB)

56.
Nanobodies: flexible structures for biotechnological applications
Ario De Marco, vabljeno predavanje na konferenci brez natisa

Najdeno v: osebi
Ključne besede: nanobodies, in vitro panning, extracellular vesicles, oncology, toxic algae
Objavljeno: 16.03.2018; Ogledov: 1208; Prenosov: 0
.pdf Polno besedilo (436,52 KB)

57.
Pre-immune nanobody collections and their biotechnological applications
Ario De Marco, predavanje na tuji univerzi

Najdeno v: osebi
Ključne besede: phage libraries, nanobodies, in vitro antibody panning
Objavljeno: 17.09.2018; Ogledov: 811; Prenosov: 0
.pdf Polno besedilo (54,45 KB)

58.
Nanobodies as convenient reagents for multiple biotechnological applications
Ario De Marco, predavanje na tuji univerzi

Najdeno v: osebi
Ključne besede: biomarker detection, immunocapture, nanobodies
Objavljeno: 17.09.2018; Ogledov: 793; Prenosov: 0
.pdf Polno besedilo (4,47 MB)

59.
In vitro and in silico approaches to obtain tailored reagents – Nanobodies as model
Ario De Marco, predavanje na tuji univerzi

Najdeno v: osebi
Ključne besede: Immunoreagents, nanobodies, in vitro panning, recombinant antibody production
Objavljeno: 21.09.2018; Ogledov: 822; Prenosov: 0
.pdf Polno besedilo (979,15 KB)

60.
A comprehensive nanobody platform to answer to diversified biotechnological needs
Ario De Marco, predavanje na tuji univerzi

Najdeno v: osebi
Ključne besede: nanobodies, phage display, fluorescent recombinant antibodies
Objavljeno: 11.10.2018; Ogledov: 885; Prenosov: 0
.pdf Polno besedilo (234,23 KB)

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