Repozitorij Univerze v Novi Gorici

Iskanje po repozitoriju
A+ | A- | Pomoč | SLO | ENG

Iskalni niz: išči po
išči po
išči po
išči po
* po starem in bolonjskem študiju

Opcije:
  Ponastavi


71 - 76 / 76
Na začetekNa prejšnjo stran12345678Na naslednjo stranNa konec
71.
Molecular dynamics simulations and docking enable to explore the biophysical factors controlling the yields of engineered nanobodies
Miguel Soler, Ario De Marco, Sara Fortuna, 2016, izvirni znanstveni članek

Opis: Nanobodies (VHHs) have proved to be valuable substitutes of conventional antibodies for molecular recognition. Their small size represents a precious advantage for rational mutagenesis based on modelling. Here we address the problem of predicting how Camelidae nanobody sequences can tolerate mutations by developing a simulation protocol based on all-atom molecular dynamics and wholemolecule docking. The method was tested on two sets of nanobodies characterized experimentally for their biophysical features. One set contained point mutations introduced to humanize a wild type sequence, in the second the CDRs were swapped between single-domain frameworks with Camelidae and human hallmarks. The method resulted in accurate scoring approaches to predict experimental yields and enabled to identify the structural modifications induced by mutations. This work is a promising tool for the in silico development of single-domain antibodies and opens the opportunity to customize single functional domains of larger macromolecules
Ključne besede: nanobodies, molecular dynamics, modeling, antibody solubility
Objavljeno v RUNG: 11.10.2016; Ogledov: 4689; Prenosov: 244
.pdf Celotno besedilo (1,95 MB)

72.
Nanobodies for research & clinical applications
Ario De Marco, predavanje na tuji univerzi

Opis: Nanobody technology evolved rapidly in the last years and now is available for applications in basic sciences as well as in diagnostics and therapy
Ključne besede: nanobodies, antibody functionalization, phage display panning, recombinant antibody production
Objavljeno v RUNG: 11.10.2016; Ogledov: 4948; Prenosov: 0
Gradivo ima več datotek! Več...

73.
NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
Sandrine Moutel, Nicolas Bery, Virginie Bernard, Laura Keller, Emilie Lemesre, Ario De Marco, Laetitia Ligat, Jean-Christophe Rain, Gilles Fevre, Aurelien Olichon, Franck Perez, 2016, izvirni znanstveni članek

Opis: In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower cost. Here, we describe the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse library provides high affinity binders without animal immunization. NaLi-H1 was screened following several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1). Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were used in various immunoassays and were often found to be functional intrabodies, enabling tracking or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein knockdown in living cells. Finally, direct selection against the surface of tumor cells produced hs2dAb directed against tumor-specific antigens further highlighting the potential use of this library for therapeutic applications.
Ključne besede: nanobodies, synthetic phage display library, in vitro panning
Objavljeno v RUNG: 17.08.2016; Ogledov: 5205; Prenosov: 243
.pdf Celotno besedilo (4,32 MB)

74.
Selection criteria and engineering of nanobodies for oncological applications
Ario De Marco, predavanje na tuji univerzi

Opis: Lecture relative to the biotechnological features of nanobodies
Ključne besede: nanobodies, phage display, antibody engineering, panning
Objavljeno v RUNG: 14.07.2016; Ogledov: 4339; Prenosov: 0
Gradivo ima več datotek! Več...

75.
76.
Computational design of customised nanobodies for biotechnological applications
Miguel Soler, Ario De Marco, Sara Fortuna, 2016, prispevek na konferenci brez natisa

Opis: In silico modeling to improve the biophysical characteristics of recombinant single-domain antibodies
Ključne besede: nanobodies, modeling, protein stability, antibody humanization, molecular dynamics
Objavljeno v RUNG: 26.04.2016; Ogledov: 4669; Prenosov: 0
Gradivo ima več datotek! Več...

Iskanje izvedeno v 0.03 sek.
Na vrh