Repository of University of Nova Gorica

Show document
A+ | A- | SLO | ENG

Title:Microfluidic flow injection thermal lens microscopy for high throughput and sensitive analysis of sub-μL samples
Authors:Liu, Mingqiang (Author)
Malovrh, Sara (Author)
Franko, Mladen (Author)
Files:.pdf Cr(VI).pdf (828,33 KB)
 
Language:English
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:An analytical method combining microfluidic flow injection analysis (μFIA) with thermal lens microscopy (TLM) was developed for high throughput and sensitive analysis of sub-μL samples. Performance of the μFIA-TLM was validated for detection of hexavalent chromium [Cr(VI)] in water samples. At different sample injection volumes, detection positions and flow rates, influences of the reaction time and the diffusion of Cr-diphenylcarbazone (DPCO) complexes on the μFIA-TLM signal were investigated. Photodegradation of the Cr-DPCO complex was clearly observed when the absorbed photons per Cr-DPCO is above 1600. After optimization of the TLM with respect to rapid flows (up to 10 cm/s), we achieved a limit of detection of 0.6 ng/mL for Cr(VI) in a 50-μm deep channel. Impacts of interfering ions [V(V), Mo(VI), Fe(III)] on the Cr(VI) determination were found to be small. Cr(VI) in real samples from a cement factory were determined and found in good agreement with results of spectrophotometry. This μFIA-TLM shows advantages over its conventional counterpart, such as eliminating additional sample conditioning, reducing over 100 times the sample consumption to sub-μL and over 10 times the time required for one sample injection to a few seconds (up to 20 samples/min). The optimized μFIA-TLM setup can be applied for fast and sensitive analysis of nonfluoresent sub-μL samples in rapidly flowing mediums.
Keywords:Spektrometrija TLS, mikroskopija TLM, mikrofluidika, krom (VI)
Year of publishing:2016
Number of pages:8
Numbering:8
COBISS_ID:4365051 Link is opened in a new window
URN:URN:SI:UNG:REP:GJHFAC3A
DOI:10.1039/C6AY00932H Link is opened in a new window
Views:1963
Downloads:130
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:Document is not linked to any category.
:
  
Average score:(0 votes)
Your score:Voting is allowed only for logged in users.

Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Record is a part of a journal

Title:Analytical methods
Shortened title:Anal. Methods
ISSN:ISSN 0003-2654
Year of publishing:2016

Back