Repozitorij Univerze v Novi Gorici

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Naslov:Bilirubin is an Endogenous Antioxidant in Human Vascular Endothelial Cells
Avtorji:Žiberna, Lovro (Avtor)
Martelanc, Mitja (Avtor)
Franko, Mladen (Avtor)
Passamonti, Sabina (Avtor)
Datoteke:.pdf srep29240.pdf (585,86 KB)
 
.zip srep29240-s1.pdf.zip (314,62 KB)
 
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:Bilirubin is a standard serum biomarker of liver function. Inexplicably, it is inversely correlated with cardiovascular disease risk. Given the role of endothelial dysfunction in originating cardiovascular diseases, direct analysis of bilirubin in the vascular endothelium would shed light on these relationships. Hence, we used high-performance liquid chromatography coupled with thermal lens spectrometric detection and diode array detection for the determination of endogenous cellular IXα-bilirubin. To confirm the isomer IXα-bilirubin, we used ultra-performance liquid chromatography coupled with a high-resolution mass spectrometer using an electrospray ionization source, as well as tandem mass spectrometric detection. We measured bilirubin in both arterial and venous rat endothelium (0.9–1.5 pmol mg−1 protein). In the human endothelial Ea.hy926 cell line, we demonstrated that intracellular bilirubin (3–5 pmol mg−1 protein) could be modulated by either extracellular bilirubin uptake, or by up-regulation of heme oxygenase-1, a cellular enzyme related to endogenous bilirubin synthesis. Moreover, we determined intracellular antioxidant activity by bilirubin, with EC50 = 11.4 ± 0.2 nM, in the range of reported values of free serum bilirubin (8.5–13.1 nM). Biliverdin showed similar antioxidant properties as bilirubin. We infer from these observations that intra-endothelial bilirubin oscillates, and may thus be a dynamic factor of the endothelial function.
Ključne besede:Antioxidants, bilirubin, endothelial cells
Leto izida:2016
Št. strani:6
Številčenje:29240, 6
COBISS_ID:4431099 Povezava se odpre v novem oknu
URN:URN:SI:UNG:REP:LMXAR9KU
DOI:10.1038/srep29240 Povezava se odpre v novem oknu
Število ogledov:1929
Število prenosov:195
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Področja:Gradivo ni uvrščeno v področja.
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Gradivo je del revije

Naslov:Scientific Reports
Skrajšan naslov:Sci. Rep.
Založnik:Nature Publishing Group: Open Access Journals - Option C
ISSN:2045-2322
Leto izida:2016

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