Repository of University of Nova Gorica

Show document
A+ | A- | SLO | ENG

Title:NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
Authors:Moutel, Sandrine (Author)
Bery, Nicolas (Author)
Bernard, Virginie (Author)
Keller, Laura (Author)
Lemesre, Emilie (Author)
de Marco, Ario (Author)
Ligat, Laetitia (Author)
Rain, Jean-Christophe (Author)
Fevre, Gilles (Author)
Olichon, Aurelien (Author)
Perez, Franck (Author)
Files:.pdf Synthetic_Library.pdf (4,32 MB)
 
Language:English
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower cost. Here, we describe the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse library provides high affinity binders without animal immunization. NaLi-H1 was screened following several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1). Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were used in various immunoassays and were often found to be functional intrabodies, enabling tracking or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein knockdown in living cells. Finally, direct selection against the surface of tumor cells produced hs2dAb directed against tumor-specific antigens further highlighting the potential use of this library for therapeutic applications.
Keywords:nanobodies, synthetic phage display library, in vitro panning
Year of publishing:2016
Number of pages:31
Numbering:5:e16228
COBISS_ID:4478971 Link is opened in a new window
URN:URN:SI:UNG:REP:LJSZXHDC
DOI:10.7554/eLife.16228 Link is opened in a new window
Views:1895
Downloads:149
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:Document is not linked to any category.
:
  
Average score:(0 votes)
Your score:Voting is allowed only for logged in users.

Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Record is a part of a journal

Title:eLIFE
Shortened title:eLIFE
Publisher:elifesciences.org
Year of publishing:2016

Back