Repozitorij Univerze v Novi Gorici

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Naslov:NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
Avtorji:Moutel, Sandrine (Avtor)
Bery, Nicolas (Avtor)
Bernard, Virginie (Avtor)
Keller, Laura (Avtor)
Lemesre, Emilie (Avtor)
de Marco, Ario (Avtor)
Ligat, Laetitia (Avtor)
Rain, Jean-Christophe (Avtor)
Fevre, Gilles (Avtor)
Olichon, Aurelien (Avtor)
Perez, Franck (Avtor)
Datoteke:.pdf Synthetic_Library.pdf (4,32 MB)
 
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower cost. Here, we describe the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse library provides high affinity binders without animal immunization. NaLi-H1 was screened following several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1). Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were used in various immunoassays and were often found to be functional intrabodies, enabling tracking or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein knockdown in living cells. Finally, direct selection against the surface of tumor cells produced hs2dAb directed against tumor-specific antigens further highlighting the potential use of this library for therapeutic applications.
Ključne besede:nanobodies, synthetic phage display library, in vitro panning
Leto izida:2016
Št. strani:31
Številčenje:5:e16228
COBISS_ID:4478971 Povezava se odpre v novem oknu
URN:URN:SI:UNG:REP:LJSZXHDC
DOI:10.7554/eLife.16228 Povezava se odpre v novem oknu
Število ogledov:1613
Število prenosov:132
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Skupna ocena:(0 glasov)
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Gradivo je del revije

Naslov:eLIFE
Skrajšan naslov:eLIFE
Založnik:elifesciences.org
Leto izida:2016

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