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Title:SYMBIOSIS ECOLOGY OF SELECTED SCYPHOZOA
Authors:Raspor Dall'Olio, Lucija (Author)
Ramšak, Andreja (Mentor) More about this mentor... New window
Malej, Alenka (Mentor) More about this mentor... New window
Files:.pdf DR_Raspor_Dall_Olio_Lucija_i2016.pdf (3,20 MB)
 
Language:English
Work type:Dissertation (m)
Tipology:2.08 - Doctoral Dissertation
Organization:FPŠ - Graduate School
Abstract:Scyphozoa with symbionts have an advantage in oligotrophic environments due to the additional source of nutrients provided by their symbiontic algae, just as corals have benefits from their symbionts. The literature, however, has thus far devoted far less attention to the association between scyphozoan hosts and Symbiodinium sp. than it does to corals. This thesis investigated the identity of symbionts from scyphozoan medusae (Cotylorhiza tuberculata, Phyllorhiza punctata and Cassiopea xamachana) using a phylogenetic approach. Two scyphozoan species, P. punctata and C. tuberculata, were sampled over the Mediterranean Sea, while Cassiopea xamachana was sampled in the Atlantic Ocean. Symbionts were identified from live medusae and their identity and phylogenetic relationships were determined by analysing two nuclear markers, ITS2 and 28S rDNA, from symbionts. Symbiodinium sp. sequences belong to clades A, B, and C based on markers ITS2 and 28S rDNA. Moreover, individual medusae host only one type Symbiodinium (A, B or C). Host species from the Mediterranean Sea hosted Symbiodinium from clade A and B (C. tuberculata) or only from clade A (Phyllorhiza punctata), while the host from the Atlantic Ocean (C. xamachana) hosted Symbiodinium from clade B or C. The phylogeography of C. tuberculata medusae was analysed using mtCO1. All the haplotypes sampled over the Mediterranean Sea were clustered together without any sign of phylogeographic structuring.
Keywords:Scyphozoa, Cotylorhiza tuberculata, Symbiodinium sp., symbiosis, Mediterranean Sea, phylogeography, CO1, 28S rDNA, ITS regions
Year of publishing:2016
Source:Nova Gorica
COBISS_ID:4522235 Link is opened in a new window
URN:URN:SI:UNG:REP:STKV655S
Views:3872
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Secondary language

Language:Slovenian
Title:Ekologija simbioze izbranih klobučnjakov
Abstract:Simbiotske meduze imajo to prednost, da v oligotrofnem okolju dobijo hranila od svojih simbionskih alg, prav tako kot korale. V primerjavi z koralno simbiozo, o simbiotski povezavi med meduzo in njenimi simbionti Symbiodinium sp. vemo precej manj. V tem doktorskem delu smo identificirali simbionte iz morske cvetače (Cotylorhiza tuberculata), Phyllorhiza punctata in Cassiopea xamachana ter preučili genetsko raznolikost tako gostitelja C. tuberculate kot simbontov. Vzorce meduz P. punctata in C. tuberculata smo nabrali na različnih lokacijah na Sredozemskem morju, razen Ca. xamachana, ki smo jo vzorčili v Atlantskem oceanu. Primerjali smo vzorce distribucije simbiontov med različnimi lokacijami. Identificirali smo jih direktno iz žive meduze z izolacijo simbiontske DNA, nato pa smo pomnožili ribosomalna genetska markerja ITS2 in 28S. Na osnovi obeh markerjev so zaporedja so pripadala kladu A, B in C. Posamezna meduza vsebuje zgolj en tip Symbiodiniuma (A, B ali C). Gostitelji, ki živijo v Sredozemskem morju so gostili Symbiodinium klada A in B (C. tuberculata) ali samo klad A (P. punctata), medtem ko v gostiteljih iz Atlantskega oceana (Ca. xamachana) najdemo Symbiodinium klada B in C. S filogenetsko analizo mitohondrijskega markerja CO1 smo določili raznolikost C. tuberculate. Vsi haplotipi so bili uvrščeni v isto skupino, brez filogeografske strukture.
Keywords:Klobučnjaki, morska cvetača, Cotylorhiza tuberculata, Symbiodinium sp., simbioza, Sredozemsko morje, filogeografija, CO1, 28S rDNA, ITS regija.


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