Repozitorij Univerze v Novi Gorici

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Naslov:The scaffold protein calcium/calmodulin-dependent serine protein kinase controls ATP release in sensory ganglia upon P2X3 receptor activation and is part of an ATP keeper complex.
Avtorji:ID Bele, Tanja, University of Nova Gorica (Avtor)
ID Fabbretti, Elsa, University of Nova Gorica (Avtor)
Datoteke: Gradivo nima datotek, ki so prostodostopne za javnost. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u. Povezava se odpre v novem oknu
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:P2X3 receptors, gated by extracellular ATP, are expressed by sensory neurons and are involved in peripheral nociception and pain sensitization. The ability of P2X3 receptors to transduce extracellular stimuli into neuronal signals critically depends on the dynamic molecular partnership with the calcium/calmodulin-dependent serine protein kinase (CASK). The present work used trigeminal sensory neurons to study the impact that activation of P2X3 receptors (evoked by the agonist α,β-meATP) has on the release of endogenous ATP and how CASK modulates this phenomenon. P2X3 receptor function was followed by ATP efflux via Pannexin1 (Panx1) hemichannels, a mechanism that was blocked by the P2X3 receptor antagonist A-317491, and by P2X3 silencing. ATP efflux was enhanced by nerve growth factor, a treatment known to potentiate P2X3 receptor function. Basal ATP efflux was not controlled by CASK, and carbenoxolone or Pannexin silencing reduced ATP release upon P2X3 receptor function. CASK-controlled ATP efflux followed P2X3 receptor activity, but not depolarization-evoked ATP release. Molecular biology experiments showed that CASK was essential for the transactivation of Panx1 upon P2X3 receptor activation. These data suggest that P2X3 receptor function controls a new type of feed-forward purinergic signaling on surrounding cells, with consequences at peripheral and spinal cord level. Thus, P2X3 receptor-mediated ATP efflux may be considered for the future development of pharmacological strategies aimed at containing neuronal sensitization. P2X3 receptors are involved in sensory transduction and associate to CASK. We have studied in primary sensory neurons the molecular mechanisms downstream P2X3 receptor activation, namely ATP release and partnership with CASK or Panx1. Our data suggest that CASK and P2X3 receptors are part of an ATP keeper complex, with important feed-forward consequences at peripheral and central level.
Ključne besede:DRG, hemichannels, pain, purinergic signaling, sensory neurons
Leto izida:2016
Št. strani:587-597
Številčenje:138, 4
PID:20.500.12556/RUNG-2942 Novo okno
COBISS.SI-ID:4641531 Novo okno
DOI:10.1111/jnc.13680 Novo okno
NUK URN:URN:SI:UNG:REP:8NKDWOYK
Datum objave v RUNG:19.01.2017
Število ogledov:5532
Število prenosov:0
Metapodatki:XML DC-XML DC-RDF
:
BELE, Tanja in FABBRETTI, Elsa, 2016, The scaffold protein calcium/calmodulin-dependent serine protein kinase controls ATP release in sensory ganglia upon P2X3 receptor activation and is part of an ATP keeper complex. Journal of Neurochemistry [na spletu]. 2016. Vol. 138, no. 4, p. 587–597. [Dostopano 7 april 2025]. DOI 10.1111/jnc.13680. Pridobljeno s: https://repozitorij.ung.si/IzpisGradiva.php?lang=slv&id=2914
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Gradivo je del revije

Naslov:Journal of Neurochemistry
Založnik:Wiley online library
Leto izida:4
ISSN:1471-4159

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