Repozitorij Univerze v Novi Gorici

Izpis gradiva
A+ | A- | Pomoč | SLO | ENG

Naslov:Electron Microscopic Visualization of Complementary Labeled DNA With Platinum-Containing Guanine Derivative
Avtorji:ID Loukanov, Alexandre, Department of Chemistry, Graduate School of Science and Engineering, Saitama University, Japan (Avtor)
ID Filipov, Chavdar, Department of Engineering Geoecology, Laboratory of Engineering NanoBiotechnology, University of Mining and Geology “St. Ivan Rilski”, Bulgaria (Avtor)
ID Mladenova, Polina, Department of Engineering Geoecology, Laboratory of Engineering NanoBiotechnology, University of Mining and Geology “St. Ivan Rilski”, Bulgaria (Avtor)
ID Toshev, Svetlin, Department of Engineering Geoecology, Laboratory of Engineering NanoBiotechnology, University of Mining and Geology “St. Ivan Rilski”, Bulgaria (Avtor)
ID Emin, Saim, Materials Research Laboratory, University of Nova Gorica, Nova Gorica, Slovenia (Avtor)
Datoteke: Gradivo nima datotek, ki so prostodostopne za javnost. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u. Povezava se odpre v novem oknu
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:The object of the present report is to provide a method for a visualization of DNA in TEM by complementary labeling of cytosine with guanine derivative, which contains platinum as contrast-enhanced heavy element. The stretched single-chain DNA was obtained by modifying double-stranded DNA. The labeling method comprises the following steps: (i) stretching and adsorption of DNA on the support film of an electron microscope grid (the hydrophobic carbon film holding negative charged DNA); (ii) complementary labeling of the cytosine bases from the stretched single-stranded DNA pieces on the support film with platinum containing guanine derivative to form base-specific hydrogen bond; and (iii) producing a magnified image of the base-specific labeled DNA. Stretched single-stranded DNA on a support film is obtained by a rapid elongation of DNA pieces on the surface between air and aqueous buffer solution. The attached platinum-containing guanine derivative serves as a high-dense marker and it can be discriminated from the surrounding background of support carbon film and visualized by use of conventional TEM observation at 100 kV accelerated voltage. This method allows examination of specific nucleic macromolecules through atom-by-atom analysis and it is promising way toward future DNA-sequencing or molecular diagnostics of nucleic acids by electron microscopic observation.
Ključne besede:DNA stretching and visualization, contrast-enhanced Pt-containing guanine, DNA sequences
Leto izida:2016
Št. strani:79
Številčenje:2016, DOI: 10.1002/jemt.22628
PID:20.500.12556/RUNG-2936-e6946265-bb78-6026-911a-5091e0d81704 Novo okno
COBISS.SI-ID:4655355 Novo okno
DOI:DOI: 10.1002/jemt.22628 Novo okno
NUK URN:URN:SI:UNG:REP:IURX3JKR
Datum objave v RUNG:02.02.2017
Število ogledov:5978
Število prenosov:0
Metapodatki:XML DC-XML DC-RDF
:
LOUKANOV, Alexandre, FILIPOV, Chavdar, MLADENOVA, Polina, TOSHEV, Svetlin in EMIN, Saim, 2016, Electron Microscopic Visualization of Complementary Labeled DNA With Platinum-Containing Guanine Derivative. MICROSCOPY RESEARCH AND TECHNIQUE [na spletu]. 2016. Vol. 2016, no. DOI: 10.1002/jemt.22628. [Dostopano 12 april 2025]. DOI DOI: 10.1002/jemt.22628. Pridobljeno s: https://repozitorij.ung.si/IzpisGradiva.php?lang=slv&id=2936
Kopiraj citat
  
Skupna ocena:
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
Objavi na:Bookmark and Share


Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše podrobnosti ali sproži prenos.

Gradivo je del revije

Naslov:MICROSCOPY RESEARCH AND TECHNIQUE
Leto izida:2016

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:01.02.2017

Nazaj