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Title:Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma Patient Derived Xenografts
Authors:Crepin, Ronan (Author)
Gentien, David (Author)
Duche, Angeline (Author)
Rapinat, Audrey (Author)
Reyes, Cecile (Author)
Nemati, Fariba (Author)
Massonnet, Gerald (Author)
Deacaudin, Didier (Author)
Djander, Selma (Author)
Moutel, Sandrine (Author)
Desrumeaux, Klervi Even (Author)
Cassoux, Nathalie (Author)
Piperno-Neumann, Sophie (Author)
Amigorena, Sebastian (Author)
Perez, Franck (Author)
Roman-Roman, Sergio (Author)
de Marco, Ario (Author)
Files:This document has no files. This document may have a phisical copy in the library of the organization, check the status via COBISS. Link is opened in a new window
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Monoclonal antibodies specific for biomarkers expressed on the surface of uveal melanoma (UM) cells would simplify the immune-capture and genomic characterization of heterogeneous tumor cells originated from patient derived xenografts (PDXs). Antibodies against four independent tumor antigens were isolated by panning a nanobody synthetic library. Such antibodies enabled flow-cytometry-based sorting of distinct cell sub-populations from UM PDXs and to analyze their genomic features. The complexity and specificity of the biochemical and genomic biomarker combinations mirrored the UM tumor polyclonality. The data showed that MUC18 is highly and universally displayed at the surface of UM cells with different genetic background and consequently represents a reliable pan-biomarker for their identification and purification. In contrast, the other three biomarkers were detected in very variable combinations in UM PDX cells. The availability of the identified nanobodies will be instrumental in developing clone-specific drug evaluation and rational clinical strategies based on accurate genomic profiling
Keywords:nanobodies, uveal melanoma, patient derived xenografts, MUC18, membrane surface biomarkers, panning, tumor polyclonality
Year of publishing:2017
Number of pages:11
COBISS_ID:4768763 Link is opened in a new window
DOI:10.1111/pcmr.12577 Link is opened in a new window
License:CC BY-NC-ND 4.0
This work is available under this license: Creative Commons Attribution Non-Commercial No Derivatives 4.0 International
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Title:Pigment Cell and Melanoma Research
Publisher:Wiley: 12 months
Year of publishing:2017