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Title:Nanobodies: towards rational design of immune-reagents
Authors:De Marco, Ario (Author)
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Language:English
Work type:Not categorized (r6)
Tipology:1.10 - Published Scientific Conference Contribution Abstract (invited lecture)
Organization:UNG - University of Nova Gorica
Abstract:Antibodies are irreplaceable reagents in both research and clinical practice. Despite their relevance, the structural complexity of conventional mono- and polyclonal antibodies (IgG) has always been a limit for their engineering towards reagents optimized for specific applications, such as in vivo diagnostics and therapy. Furthermore, their isolation is time consuming, their production expensive, and their functionalization results often in heterogeneous macromolecule populations. These drawbacks promoted the search for both innovative antibody isolation strategies and alternative scaffolds. In vitro panning of pre-immune collections of recombinant antibody fragments allows for the simple and fast recovery of binders. Since they did not undergo somatic maturation, their affinity for targets can be insufficient but on the other hand they can be rapidly mutated by standard molecular biology techniques to generate second-generation antibodies among which to identify clones with improved characteristics. Both stochastic and rational methods have been proposed for the optimization process. Random mutagenesis followed by panning at stringent conditions has been successful used to select binders with improved physical characteristics. Rational methods try to identify in silico key residues involved in the regulation of specific antibody features, such as stability or binding affinity. The accuracy of these methods usually depends on the calculation resources. In this perspective, smaller molecules can be analyzed “better” than larger because of their restricted number of residues. Nanobodies small dimensions have been long appreciated since enable better tissue penetration, shorter clearance time, higher yields. Now it becomes evident that this characteristic makes them also optimal objects for modeling.
Keywords:recombinant antibody modeling, nanobody engineering, molecular dynamics and docking
Year of publishing:2017
COBISS_ID:4954619 Link is opened in a new window
URN:URN:SI:UNG:REP:FOUFFON5
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Record is a part of a monograph

Title:Serbian Biochemical Society - Seventh Conference
Subtitle:Biochemistry of Control in Life and Technology
Conference organizer:Serbian Biochemical Society
Place of publishing:Belgrade
Year of publishing:2017
Editors:Ivan Spasojevic

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