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Title:Odziv mikrobne združbe na mešanico nevarnih spojin
Authors:Orel, Neža (Author)
Turk, Valentina (Mentor) More about this mentor... New window
Files:.pdf Neza_Orel.pdf (1,82 MB)
 
Language:Slovenian
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:FZO - Faculty of Environmental Sciences
Abstract:Morske bakterije so najbolj številčni organizmi v morskem ekosistemu in imajo pomembno vlogo v biogeokemičnih procesih kroženja snovi. Preko različnih točkovnih in netočkovnih virov v morsko okolje vstopajo onesnažila, ki predstavljajo tveganje za zdravje ljudi, morskih organizmov in povzročajo spremembe ekosistema. Na podlagi raznolike občutljivosti mikroorganizmov na onesnaženje nam odziv bakterijske združbe lahko služi kot pokazatelj stanja v okolju. V tej študiji je bil opravljen laboratorijski eksperiment v zaprtem sistemu, z namenom preverjanja interakcij med onesnažili in mikroorganizmi v morski vodi Tržaškega zaliva. Naravna mikrobna združba je bila izpostavljena dvema mešanicama kemikalij v koncentracijah, ki jih Evropska zakonodaja predpisuje kot varne ter mešanici z 10-krat povišanimi koncentracijami. S pomočjo mikrobioloških analiz za določanje bakterijskega števila, hitrosti rasti bakterijske populacije ter z analizami bakterijske združbe smo želeli ugotoviti kolikšen je njihov vpliv na naravno mikrobno združbo v dveh različnih obdobjih (poleti, spomladi). Rezultati kažejo, da je dodatek onesnažil omogočil hitro rast bakterij in porast v številu. Bakterijsko število je bilo nižje samo v vzorcih izpostavljenih onesnažilom pri nižjih koncentracijah predpisanih mejnih vrednosti kot v kontrolnem vzorcu morske vode. V vzorcih izpostavljenih onesnažilom smo zaznali predvsem povišano rast bakterij predstavnikov družine Alteromonadaceae, medtem ko so bili nekateri predstavniki bakterij družine Rhodobacteraceae inhibirani. Rezultati so potrdili potencialno uporabo mikrobioloških analiz za zaznavanje sprememb, ki jih povzroči izpostavljenost naravne bakterijske združbe mešanici nevarnih kemikalij, ter opozorili na vprašanje dopustnih mejnih vrednosti predpisanih koncentracij onesnažil.
Keywords:Onesnaženje morja, toksičnost, mikrobiologija morja, bakterijska združba, prednostna lista nevarnih kemikalij
Year of publishing:2017
Source:Nova Gorica
COBISS_ID:4969467  Link is opened in a new window
URN:URN:SI:UNG:REP:SMUHNPT7
Views:1136
Downloads:98
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Secondary language

Language:English
Title:Response of the microbial community to a mixture of hazardous compounds
Abstract:Marine bacteria are the most abundant organisms and have an important role in biogeochemical cycles in marine ecosystems.. Pollutants enter the marine environment through different point and non-point sources, representing risk to human health, marine animals and causing changes in the ecosystem. Based on their diverse sensitivity, microorganisms can serve as indicators of environmental changes. Within this study, a laboratory experiments in bach culture were performed, in order to determine interactions between pollutants and naturale population of microorganisms in the seawater of the Gulf of Trieste. Natural microbial community was exposed to two different mixtures of chemicals in safe limit concentrations, prescribed by European legislation and to one mixture with 10 times higher concentrations. By using classical and moleculare microbiological methods for determination of bacterial abundance, bacterial production and community structure analyses, we tested the impact of chemical mixtures on natural microbial community in two different seasons (summer, spring). The results showed stimulated bacterial growth and abundance in all samples exposed to pollutants. Only, the bacterial abundance was lower in the samples exposed to pollutants at safe-limit concentrations in comparison to control seawater sample. Increased growth of representatives of Alteromonadaceae family was observed in the samples exposed to pollutants, while representatives of Rhodobacteraceae family were decreased. The results confirmed the potential use of microbiological methods to identify changes, caused by the exposure of the natural bacterial community to mixture of chemical pollutants and pointed out the importance to evaluate the issue of the prescribed safe-limit concentrations.
Keywords:Marine pollution, toxicity, marine microbiology, bacterial community, priority list of the hazardous chemicals


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