Title: | Hemichrome Determination by Thermal Lensing with Polyethylene Glycols for Signal Enhancement in Aqueous Solutions |
---|
Authors: | Galimova, Viktoriya (Author) Franko, Mladen (Author) Proskurnin, Mikhail (Author) Liu, Mingqiang (Author) et al. |
---|
Files: | This document has no files. This document may have a phisical copy in the library of the organization, check the status via COBISS.  |
---|
Language: | English |
---|
Work type: | Not categorized (r6) |
---|
Tipology: | 1.01 - Original Scientific Article |
---|
Organization: | UNG - University of Nova Gorica |
---|
Abstract: | The thermal lens technique is proposed for the determination of total hemoglobin in the form of reversible hemichrome. The conditions were optimized (concentration of sodium dodecyl sulfate, 2 mM) to attain the maximum sensitivity with the use of polyethylene glycols as signal enhancers. For polyethylene glycols with molecular weights 1500–35000 Da in a concentration range of 5–15% w/w (5–25 mM), the influence on thermal lens signal enhancement was estimated. It is shown that the use of 5% w/w polyethylene glycol 2000 provides the maximum increase in the thermal lens enhancement factor (by 40%) in comparison with unmodified aqueous solutions. The detection limit of iron(II) tris(1,10-phenanthrolinate) as a model system is 60 nM. Under these conditions, the thermal lens detection limit of hemichrome is 10 nM, which shows a 15-fold enhancement compared to spectro- photometry. Modification of the medium with polyethylene glycols decreases the limit of detection of hemichrome determination by 15% in comparison with unmodified aqueous solutions due to better reproducibility for the range of concentrations from 0.02 to 0.9 μM. |
---|
Keywords: | Hemoglobin, hemikrom, optotermična spektroskopija, etilenglikol, spektrometrija s toplotnimi lečami |
---|
Year of publishing: | 2018 |
---|
Number of pages: | 1743-1762 |
---|
Numbering: | 11, 51 |
---|
COBISS_ID: | 5136891  |
---|
URN: | URN:SI:UNG:REP:LX3R9ASK |
---|
DOI: | 10.1080/00032719.2017.1391828  |
---|
License: |  This work is available under this license: Creative Commons Attribution Non-Commercial No Derivatives 4.0 International |
---|
Views: | 1528 |
---|
Downloads: | 0 |
---|
Metadata: |  |
---|
Categories: | Document is not linked to any category. |
---|
:
|
|
---|
| | | Average score: | (0 votes) |
---|
Your score: | Voting is allowed only for logged in users. |
---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |