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Naslov:A NEW TOOL TO EVALUATE CORTISOL CONCENTRATIONS IN ANIMAL HAIR
Avtorji:Comin, Antonella (Avtor)
Peric, Tanja (Avtor)
Colzani, Mara (Avtor)
De Vecchi, Francesco (Avtor)
Bergamin, Cristina (Avtor)
Prandi, Alberto (Avtor)
Datoteke:Gradivo nima datotek. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u. Povezava se odpre v novem oknu
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.12 - Objavljeni povzetek znanstvenega prispevka na konferenci
Organizacija:UNG - Univerza v Novi Gorici
Opis:The study of cortisol in hair requires the use of a highly sensitive assay. To date, hair cortisol concentrations (HCC) have been evaluated with different analytical methods as Radio Immune Assay (RIA), Enzyme Immunossay (EIA), Enzyme Linked Immunosorbent Assay (ELISA), ChemiLuminescent Immunoassay (CLIA) and High Performance Liquid Chromatography with Mass Spectrometry (HPLC/MS) or Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS). Amplified luminescent proximity homogeneous assay (AlphaLISA) is an ELISA-like, nonradioactive technology first reported in 1994 [1]. This technology allows the quantitative detection of molecules of interest in a light-induced chemiluminescence immunoassay using a microplate without wash step. The aim of the study was to compare RIA with AlphaLISA method and examine the sensitivity in evaluating cortisol concentrations in animal hair of the Cortisol AlphaLISA kit (PerkinElmer, USA), originally suggested for the analysis of buffer and serum samples. The study has been carried out on 4 samples of calf, 4 samples of foal and 8 samples of sheep hair. After washing with isopropanol, two extracts were obtained from each hair sample. One extract has been evaluated for HCC by RIA as previously described [2,3]. The second extract has been reconstituted with the buffer provided by the Cortisol AlphaLISA Kit and HCC evaluated by the use of this commercially available kit. The preliminary results indicate that the Cortisol AlphaLISA Kit is capable to detect cortisol also in hair samples with high sensitivity, with a detection limit of 17 pg/ml. Intra- and inter-assay coefficients of variation (CVs) were 4.0% and 9.0%, respectively. Cortisol concentrations ranged between 1.31 and 16.94 pg/mg, 6.30 and 57.65 pg/mg, and 2.16 and 45.08 in calf, foal and sheep hair, respectively. The HCC obtained by Cortisol AlphaLISA Kit and RIA showed a good correlation (r=0.79, p<0.01). Considering the possibility to use a low amount of extracted sample, its no-washing procedure and the performances showed, we can conclude that the Cortisol AlphaLISA Kit can be considered an excellent tool to evaluate cortisol concentrations also in hair derived from animal species.
Ključne besede:hair, cortisol, analyses, method, AlphaLISA, Amplified luminescent proximity homogeneous assay
Leto izida:2018
Št. strani:1
COBISS_ID:5369851 Povezava se odpre v novem oknu
URN:URN:SI:UNG:REP:L5JQXNNT
Število ogledov:31
Število prenosov:0
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
Področja:Gradivo ni uvrščeno v področja.
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Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.

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Gradivo je del monografije

Naslov:Proceedings of the joint meeting LXXII Convegno S.I.S.Vet, XVIII Convegno S.I.C.V., XVI Convegno S.I.R.A., XV Convegno A.I.P.Vet, XV Giornata studio So.Fi.Vet., V Convegno R.N.I.V., Torino, Italy, 20-22 June, 2018
Prireditelj konference:University of Turin, Italy
ISBN:978-8890909214
Kraj izida:Torino
Leto izida:2018

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