Repozitorij Univerze v Novi Gorici

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Naslov:TD/GC–MS analysis of volatile markers emitted from mono- and co-cultures of Enterobacter cloacae and Pseudomonas aeruginosa in artificial sputum
Avtorji:White, Iain R (Avtor)
Lawal, Oluwasola (Avtor)
knobel, Hugo (Avtor)
Hans, Weda (Avtor)
Nijsen, Tamara M E (Avtor)
Goodacre, Royston (Avtor)
Fowler, Stephen J (Avtor)
Ahmed, Waqar M (Avtor)
Artigas, Antonio (Avtor)
Barnard-Smith, Jonathan (Avtor)
Bos, Lieuwe D (Avtor)
Camprubi, Marta (Avtor)
Coelho, Luis (Avtor)
Dark, Paul (Avtor)
Davie, Alan (Avtor)
Diaz, Emili (Avtor)
Goma, Gemma (Avtor)
Felton, Timothy (Avtor)
Leopold, Jan H (Avtor)
van Oort, Pouline M P (Avtor)
Povoa, Pedro (Avtor)
Portsmouth, Craig (Avtor)
et al.
Datoteke:.pdf Lawal2018_Article_TDGCMSAnalysisOfVolatileMarker.pdf (1,29 MB)
 
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:Introduction: Infections such as ventilator-associated pneumonia (VAP) can be caused by one or more pathogens. Current methods for identifying these pathogenic microbes often require invasive sampling, and can be time consuming, due to the requirement for prolonged cultural enrichment along with selective and differential plating steps. This results in delays in diagnosis which in such critically ill patients can have potentially life-threatening consequences. Therefore, a non-invasive and timely diagnostic method is required. Detection of microbial volatile organic compounds (VOCs) in exhaled breath is proposed as an alternative method for identifying these pathogens and may distinguish between mono- and poly-microbial infections. Objectives: To investigate volatile metabolites that discriminate between bacterial mono- and co-cultures. Methods: VAP-associated pathogens Enterobacter cloacae and Pseudomonas aeruginosa were cultured individually and together in artificial sputum medium for 24 h and their headspace was analysed for potential discriminatory VOCs by thermal desorption gas chromatography–mass spectrometry. Results: Of the 70 VOCs putatively identified, 23 were found to significantly increase during bacterial culture (i.e. likely to be released during metabolism) and 13 decreased (i.e. likely consumed during metabolism). The other VOCs showed no transformation (similar concentrations observed as in the medium). Bacteria-specific VOCs including 2-methyl-1-propanol, 2-phenylethanol, and 3-methyl-1-butanol were observed in the headspace of axenic cultures of E. cloacae, and methyl 2-ethylhexanoate in the headspace of P. aeruginosa cultures which is novel to this investigation. Previously reported VOCs 1-undecene and pyrrole were also detected. The metabolites 2-methylbutyl acetate and methyl 2-methylbutyrate, which are reported to exhibit antimicrobial activity, were elevated in co-culture only. Conclusion: The observed VOCs were able to differentiate axenic and co-cultures. Validation of these markers in exhaled breath specimens could prove useful for timely pathogen identification and infection type diagnosis.
Ključne besede:Bacteria, Enterobacter cloacae, Gas Chromatography-Mass Spectrometry, Infection, Pseudomonas aeruginosa, Volatile organic compounds
Leto izida:2018
Št. strani:11
Številčenje:14, 5
COBISS_ID:5419515 Povezava se odpre v novem oknu
URN:URN:SI:UNG:REP:U3BSB50K
DOI:10.1007/s11306-018-1357-5 Povezava se odpre v novem oknu
Licenca:CC BY-SA 4.0
To delo je dosegljivo pod licenco Creative Commons Priznanje avtorstva-Deljenje pod enakimi pogoji 4.0 Mednarodna
Število ogledov:169
Število prenosov:9
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Področja:Gradivo ni uvrščeno v področja.
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Gradivo je del revije

Naslov:Metabolomics
Skrajšan naslov:Metabolomics
Založnik:Springer Nature
ISSN:15733882
Leto izida:2018

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