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Naslov:Exhaled breath metabolomics reveals a pathogen-specific response in a rat pneumonia model for two human pathogenic bacteria: a proof-of-concept study
Avtorji:ID van Oort, Pouline M, Department of Intensive Care, Amsterdam University Medical Center–Academic Medical Centre, Amsterdam, The Netherlands (Avtor)
ID White, Iain R., Manchester Institute of Biotechnology, Manchester, United Kingdom (Avtor), et al.
Datoteke: Gradivo nima datotek, ki so prostodostopne za javnost. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u. Povezava se odpre v novem oknu
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:Volatile organic compounds in breath can reflect host and pathogen metabolism and might be used to diagnose pneumonia. We hypothesized that rats with Streptococcus pneumoniae (SP) or Pseudomonas aeruginosa (PA) pneumonia can be discriminated from uninfected controls by thermal desorption-gas chromatography-mass-spectrometry (TD-GC-MS) and selected ion flow tube-mass spectrometry (SIFT-MS) of exhaled breath. Male adult rats (n = 50) received an intratracheal inoculation of 1) 200 µl saline, or 2) 1 × 107 colony-forming units of SP or 3) 1 × 107 CFU of PA. Twenty-four hours later the rats were anaesthetized, tracheotomized, and mechanically ventilated. Exhaled breath was analyzed via TD-GC-MS and SIFT-MS. Area under the receiver operating characteristic curves (AUROCCs) and correct classification rate (CCRs) were calculated after leave-one-out cross-validation of sparse partial least squares-discriminant analysis. Analysis of GC-MS data showed an AUROCC (95% confidence interval) of 0.85 (0.73-0.96) and CCR of 94.6% for infected versus noninfected animals, AUROCC of 0.98 (0.94-1) and CCR of 99.9% for SP versus PA, 0.92 (0.83-1.00), CCR of 98.1% for SP versus controls and 0.97 (0.92-1.00), and CCR of 99.9% for PA versus controls. For these comparisons the SIFT-MS data showed AUROCCs of 0.54, 0.89, 0.63, and 0.79, respectively. Exhaled breath analysis discriminated between respiratory infection and no infection but with even better accuracy between specific pathogens. Future clinical studies should not only focus on the presence of respiratory infection but also on the discrimination between specific pathogens.
Ključne besede:biomarkers, exhaled breath analysis, infection, pneumonia
Leto izida:2019
Št. strani:751-756
Številčenje:5, 316
PID:20.500.12556/RUNG-4666-f96e3771-3df4-e023-dc58-14918e53e487 Novo okno
COBISS.SI-ID:5424379 Novo okno
DOI:10.1152/ajplung.00449.2018 Novo okno
NUK URN:URN:SI:UNG:REP:WLDGHVP7
Datum objave v RUNG:22.07.2019
Število ogledov:4392
Število prenosov:0
Metapodatki:XML DC-XML DC-RDF
:
VAN OORT, Pouline M in WHITE, Iain R., 2019, Exhaled breath metabolomics reveals a pathogen-specific response in a rat pneumonia model for two human pathogenic bacteria: a proof-of-concept study. American Journal of Physiology-Lung Cellular and Molecular Physiology [na spletu]. 2019. Vol. 5, no. 316, p. 751–756. [Dostopano 7 april 2025]. DOI 10.1152/ajplung.00449.2018. Pridobljeno s: https://repozitorij.ung.si/IzpisGradiva.php?lang=slv&id=4666
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Gradivo je del revije

Naslov:American Journal of Physiology-Lung Cellular and Molecular Physiology
Skrajšan naslov:AJP-LUNG
Založnik:American Physiological Society
Leto izida:2019
ISSN:10400605

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