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Title:Fibroblast protein profile analysis highlights the role of oxidative stress and vitamin K recycling in the pathogenesis of pseudoxanthoma elasticum.
Authors:Boraldi, Federica (Author)
Annovi, Giulia (Author)
Guerra, Deanna (Author)
Chiara, Paolinelli Devincenzi (Author)
Maria Immaculada, Garcia Fernandez (Author)
Panico, Fulvio (Author)
De Santis, Giorgio (Author)
Tiozzo, Roberta (Author)
Pasquali Ronchetti, Ivonne (Author)
Quaglino, Daniela (Author)
Files:This document has no files. This document may have a phisical copy in the library of the organization, check the status via COBISS. Link is opened in a new window
Language:English
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Pseudoxanthoma elasticum (PXE) is a genetic disorder associated to mutations in the ABCC6 gene; however, the pathogenetic mechanisms leading to elastic fibre calcifications and to clinical manifestations are still unknown. Dermal fibroblasts, directly involved in the production of the extracellular milieu, have been isolated from healthy subjects and from patients affected by PXE, cultured in vitro and characterized for their ability to produce reactive oxygen species, for structural and functional properties of their cell membranes, for changes in their protein profile. Data demonstrate that oxidative stress has profound and endurable consequences on PXE fibroblast phenotype being responsible for: reduced levels of global DNA methylation, increased amount of carbonylated proteins and of lipid peroxidation products, altered structural properties of cell membranes, modified protein expression. Data shed new light on the pathogenetic pathways in PXE, by identifying a network of proteins affecting elastic fibre calcification through inefficient vitamin K recycling, and highlight the role of differentially expressed proteins as targets for validating the efficacy of future therapeutic strategies aiming to delay and/or revert the pathologic phenotype of PXE fibroblasts. Moreover, data open new perspectives for investigating PXE-like phenotypes in the absence of ABCC6 mutations.
Keywords:Ectopic calcification / Elastin / Fibroblast proteome / MRP6 / PXE
Year of publishing:2009
Number of pages:1084-1098
Numbering:3
COBISS_ID:5440507  Link is opened in a new window
URN:URN:SI:UNG:REP:AJAK4E7V
DOI:10.1002/prca.200900007 Link is opened in a new window
License:CC BY-NC-ND 4.0
This work is available under this license: Creative Commons Attribution Non-Commercial No Derivatives 4.0 International
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Record is a part of a journal

Title:Proteomics Clinical Apllication
Year of publishing:2009

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