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Title:A microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute wounds of human skin: An exploratory study
Authors:Ashrafi, Mohammed (Author)
Xu, Yun (Author)
Muhamadali, Howbeer (Author)
White, Iain R (Author)
Wilkinson, Maxim (Author)
Baguneid, Mohamed (Author)
Goodacre, Roy (Author)
Bayat, Ardeshir (Author)
et al.
Files:This document has no files. This document may have a phisical copy in the library of the organization, check the status via COBISS. Link is opened in a new window
Language:English
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Profiling skin microbiome and metabolome has been utilised to gain further insight into wound healing processes. The aims of this multi-part temporal study in 11 volunteers were to analytically profile the dynamic wound tissue and headspace metabolome and sequence microbial communities in acute wound healing at days 0, 7, 14, 21 and 28, and to investigate their relationship to wound healing, using non-invasive quantitative devices. Metabolites were obtained using tissue extraction, sorbent and polydimethylsiloxane patches and analysed using GCMS. PCA of wound tissue metabolome clearly separated time points with 10 metabolites of 346 being involved in separation. Analysis of variance-simultaneous component analysis identified a statistical difference between the wound headspace metabolome, sites (P = 0.0024) and time points (P<0.0001), with 10 out of the 129 metabolites measured involved with this separation between sites and time points. A reciprocal relationship between Staphylococcus spp. and Propionibacterium spp. was observed at day 21 (P<0.05) with a statistical correlation between collagen and Propionibacterium (r = 0.417; P = 0.038) and Staphylococcus (r = -0.434; P = 0.03). Procrustes analysis showed a statistically significant similarity between wound headspace and tissue metabolome with non-invasive wound devices. This exploratory study demonstrates the temporal and dynamic nature of acute wound metabolome and microbiome presenting a novel class of biomarkers that correspond to wound healing, with further confirmatory studies now necessary.
Keywords:metabolomics, skin, volatile organic compounds, VOCs, wound healing
Year of publishing:2020
Number of pages:26
Numbering:2, 15
COBISS_ID:5581307 Link is opened in a new window
URN:URN:SI:UNG:REP:NEFAJ0IG
DOI:10.1371/journal.pone.0229545 Link is opened in a new window
License:CC BY-NC-SA 4.0
This work is available under this license: Creative Commons Attribution Non-Commercial Share Alike 4.0 International
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Record is a part of a journal

Title:PLoS ONE
Shortened title:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
Year of publishing:2020

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